Four-laser, 10-color benchtop flow cytometer
High Throughput Sampler
Laser: 488nm, 355nm, 405 nm, 633 nm
left cover right coverside door
control panel SIP power switch
fluidicsinterconnects
Control Panel
SAMPLEFINE ADJ
LO MED HI
RUN STNDBY PRIME
sample flow ratefluid control buttons
fluid control buttons
Sample flow rates: approximately 12, 35, and 60 μL/min ( LO, MED, and HI settings )
355 nm (UV) : Indo-1, DAPI, Alexa Fluor 350405 nm (Violet) : CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405488 nm (Blue) : FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680
bandpass filters
longpass dichroic mirrors
Octagon Detector Arrays
488-nm blue laser
PE-Cy5
633-nm red laser
660/20
APC
735 LP
780/60
APC-Cy7
670/14
PerCP-Cy5.5
635 LP
FITC530/30505 LP
PE-Cy7780/60
735 LP
PE
575/26
SS
C488/10
PerCP
GFP
PE-Cy5
695/40BP685LP
PI
355-nm UV laser
450/50
505 LP
530/30
DAPI
Indo-1 (Blue)
405-nm violet laser
440/40
Pacific Blue
505 LP
525/50
AmCyan
Alexa405
Alexa350
CascadeB
CascadeY
To Login the computer
Login Name: flow user Password: fccf
shortcut icon
To start the software, double click the shortcut icon on the desktop
Login to FACSDiva Software
Preparing Sheath and Waste Containers
Fluidics CleaningEach time you finish using the cytometer, clean the sample injection tube and the area between the injection tube and the outer sleeve. This prevents the sample injection tube from becoming clogged and removes dyes that can remain in the tubing. CLEANING INSTRUCTIONS ARE POSTED ON THE LSRII
LSRII Location:
BioSquare III, 670 Albany St. 5th Floor
(Card access is required to enter the room)
FCCF web address:
http://www.bu.edu/cores/flow-cytometry
FCCF email:
Sorting facility phone number:
617-414-5225