Download - Josh Powell virus research
Engineer “Arg-‐Gly-‐Asp” (RGD)
RGD: stem-‐cell
adhesion epitope also target cancer
cells that up-‐regulate integrin
TYMV with RGD subsDtuDon at residue 44
Wild-‐type
N-‐term: Mutant
pH-‐dependent enhanced stability Mutate the internal scaffold N-‐terminus of CP
Robust TYMV virus-‐like parDcles in E. coli
In vitro reassembly from a denatured
state
AMer many months crystal structure data for TYMV and
mutants!
TYMV can form a molecular “container” aMer release of RNA, nano-‐inspired applicaDons with a protein shell with a small “hole”
AMer freeze-‐thaw
before
TYMV dual-‐subgenomic RNA for expression of GFP and GUS. Future downstream use for vaccine development
Biophysical studies of TYMV CharacterizaDon empty, virion and intermediate parDcles
Northerns, Westerns, gradient centrifugaDon, etc.
Previous Work (masters): Signaling of a Cytomegalovirus (CMV) chemokine receptor US28: plenty of cloning, Dssue culture, immuno-‐precipitaDon/fluorescence and construcDng CMV knockout mutants
US28 chemokine
Recent TYMV arDcle & (cover image) Jan 2011, more publicaDons to come!
Publish or Perish!!!!!