Download - Laboratory safety
![Page 1: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/1.jpg)
Laboratory safety
Prepared bySamira fattah
Assis. Lec.College of health sciences-HMU
Lab. 1
![Page 2: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/2.jpg)
Introduction
• Laboratory safety rules are a major aspect of every microbiology lab.
• Each student in microbiology laboratory must follow specific safety rules and procedures.
![Page 3: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/3.jpg)
Why is Lab Safety Important?
• Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.
![Page 4: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/4.jpg)
Lab Safety Rules
![Page 5: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/5.jpg)
Lab Safety Rules1-Wear protective clothing .
![Page 6: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/6.jpg)
Protective clothes
o Gloves are essential.
o Lab coats are required.
o Safety glasses ( goggles) may be
required to avoid splashes.
![Page 7: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/7.jpg)
2-Laboratory personnel should not wear sandals
Lab Safety Rules
![Page 8: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/8.jpg)
Lab Safety RulesDo NOT Wear:
• Jewelry
• Loose or Baggy clothing
![Page 9: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/9.jpg)
3-Avoid touching objects (e.g., pencils, cell phones, door handles) while wearing gloves.
Lab Safety Rules
![Page 10: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/10.jpg)
4-Pencils, labels, or any other materials should never be placed in your mouth.
![Page 11: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/11.jpg)
5-Caution must be taken when using gas burners. Be sure gas burners are turned off when finished.
![Page 12: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/12.jpg)
6-Long hair must be tied back or covered to minimize fire hazard or contamination of experiments.
Lab Safety Rules
![Page 13: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/13.jpg)
Lab Safety Rules7- Do not eat food or drink water in the lab. do not use lab glassware as food or water containers.
![Page 14: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/14.jpg)
Lab Safety Rules8- Protect your hands safety: - wash hands after every lab.- Handle glassware, sharp tools and heated containers carefully.
![Page 15: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/15.jpg)
Lab Safety Rules9- electrical safety: - unplug electrical equipment after use. - keep all electrical cords and wires away from water .
![Page 16: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/16.jpg)
Lab Safety Rules 10- Chemical safety: -never touch, taste or smell a chemical unless instructed to do
so. - never mix chemicals unless instructed to do so. -keep lids on chemical containers when not in use.
![Page 17: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/17.jpg)
Lab Safety Rules11-Do not take any cultures out of the
lab for any reason.• All cultures should be handled as
potentially pathogenic.• Liquid cultures must always be
kept in a test tube rack.
![Page 18: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/18.jpg)
Lab Safety Rules 12- Do not engage in practical jokes or horseplay in the
lab.
![Page 19: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/19.jpg)
Lab Safety Rules13-Keep nonessential books and clothing far away from
your work area.
![Page 20: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/20.jpg)
Lab Safety Rules 14-Wipe the bench tops down with disinfectant both before you
begin your work and after you have completed your work.
![Page 21: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/21.jpg)
Lab Safety Rules 15- Dispose of waste products according to instructions.
![Page 22: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/22.jpg)
16-Report all accidents, no matter how minor, to your supervisor!!
Lab Safety Rules
![Page 23: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/23.jpg)
Lab Safety Equipment
![Page 24: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/24.jpg)
Lab Safety Equipment• Safety shower
• Eye wash
![Page 25: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/25.jpg)
Lab Safety Equipment
• Fire Blanket
• Fire Extinguisher
![Page 26: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/26.jpg)
Lab Safety Symbols
![Page 27: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/27.jpg)
Lab Safety Symbols• They alert about the possible dangers in the lab.
![Page 28: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/28.jpg)
Biological Safety Levels
![Page 29: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/29.jpg)
Biological Safety Levels
1. BSL12. BSL23. BSL34. BSL4
![Page 30: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/30.jpg)
BSL-1
– Not known to cause disease in healthy adult humans
• Safety equipment– Minimal requirements
• Facilities– Open bench top
![Page 31: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/31.jpg)
BSL-2
– Associated with mild to moderate disease in humans
• Safety equipment– Biological Safety Cabinet and
personal protective equipment as needed.
• Facilities– BSL-1 plus the availability of a
mechanism for decontamination.
![Page 32: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/32.jpg)
BSL-3
– Associated with serious or potentially lethal disease in humans
• Safety equipment– Biological Safety Cabinet and
personal protective equipment required.
• Facilities– BSL-2 with self-closing double
door access and single-pass negative directional airflow.
![Page 33: Laboratory safety](https://reader035.vdocument.in/reader035/viewer/2022081604/58792c131a28ab7c448b58d5/html5/thumbnails/33.jpg)
BSL-4
– Associated with high risk of life-threatening disease in humans and/or animals
• Safety equipment– Biological Safety Cabinet – Full-body air-supplied, positive
pressure personnel suit • Facilities
– BSL-3 plus dedicated air and exhaust, decontamination procedures for exit, separate building, etc.