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Lecture 5: Microscopy
PHYS 430/603 material
Laszlo Takacs
UMBC Department of Physics
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Light microscopy
The principle and a commercial scope
Useful sites:
http://www.microscopyu.com/articles/optics/components.htmlhttp://em-outreach.ucsd.edu/web-course/toccontents.html
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Optical microscope (OM) and transmission electron microscope (TEM)
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TEM modes:Composite image, bright field image, dark field image, selected area diffraction
Transmitted and diffracted beams Diffraction patternFirst image
Bright field Bright field, transmitted electrons only Dark field Selected area diffraction pattern
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Comparing imaging and diffraction in a TEM.
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Electron diffraction from a
monocrystal polycrystal glass
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How do scanning microscopies work?
0 2 1 2 2 1 1 1
1 9 7 2 1 8 7 0
2 8 6 1 2 9 8 1
0 8 8 1 8 7 0 1
1 9 7 9 6 0 1 1
1 7 8 8 7 1 1 0
2 9 8 8 6 1 2 1
1 8 9 2 9 7 1 1
0 8 7 1 1 8 3 1
2 1 1 0 2 1 0 1
Image = table of numbers
Measurement generates a value for every location:
• Reflectivity of light (scanner)
• Ejected electrons (SEM)
• Current between tip and surface (STM)
• Force between tip and surface (AFM)
• Any quantity of interest that can produce useful contrast
Interpret numbers as intensitiesfor display or printer.Digital image processing.
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How do scanning microscopies work?
0 2 1 2 2 1 1 1
1 9 7 2 1 8 7 0
2 8 6 1 2 9 8 1
0 8 8 1 8 7 0 1
1 9 7 9 6 0 1 1
1 7 8 8 7 1 1 0
2 9 8 8 6 1 2 1
1 8 9 2 9 7 1 1
0 8 7 1 1 8 3 1
2 1 1 0 2 1 0 1
Image = table of numbers
Measurement generates a value for every location:
• Reflectivity of light (scanner)
• Ejected electrons (SEM)
• Current between tip and surface (STM)
• Force between tip and surface (AFM)
• Any quantity of interest
Interpret numbers as intensitiesfor display or printer.Digital image processing.
![Page 9: Lecture 5: Microscopy PHYS 430/603 material Laszlo Takacs UMBC Department of Physics](https://reader035.vdocument.in/reader035/viewer/2022062409/56649eea5503460f94bfbc9a/html5/thumbnails/9.jpg)
The principle of the SEM.
There is no image formation in the optical sense.
This is classical analog system. Modern SEMs record the measured intensities in a computer memory rather then project them directly on a CRT screen. This way image processing is possible before the final image is created. TV does the same.
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Measurable effect caused by high-energy electrons
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Typical morphological contrast by secondary electrons.
It only looks like an illuminated landscape. The contrast comes from how many secondary electrons are generated and how efficiently they are collected by the detector. The illumination comes from above, the detector is n the side.
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Magnifications: very different features are seen on different length scales
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Depth of field
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X-ray analysis
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Images of a Lunar rock:
(a) Backscattered electrons(depend on Z)
(c) Fe X-rays(d) P X-rays(b) Sketch of phases;
m = metaltr = trolite, FeSsc = Fe-Ni phosphidewh = phosphate
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The principle of STM/AFM