LIMOR MAN M.D, M.MED.SC
ASSISTANT PROFESSOR OF RESEARCH IN OBSTETRICS AND GYNECOLOGY
RONALD O. PERELMAN AND CLAUDIA COHEN CENTER FOR REPRODUCTIVE MEDICINE
WEILL CORNELL MEDICINE, NYC, NY, USA
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•
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• MINIMIZE ISCHEMIC INJURY TO THE
TRANSPLANT BY REDUCING THE LATENCY
PERIOD BEFORE NEOVASCULARIZATION
IMPROVE SURVIVAL
•
-Baird DT, Campbell B, de Souza C, Telfer E. Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips. Eur J Obstet Gynecol Reprod Biol. 2004 Apr 5;113 Suppl 1:S55-9.
•
-Dolmans MM, Martinez-Madrid B, Gadisseux E, Guiot Y, Yuan WY, Torre A, Camboni A, Van Langendonckt A, Donnez J. Short-term transplantation of isolated human ovarian follicles and
cortical tissue into nude mice. Reproduction. 2007 Aug;134(2):253-62.
0
20
40
60
80
100
InVIVO Postgra ing
%Primordialfollicles
primordial primary preantral antral
AMH AMH
FSH FSH
Metabolic demands
-Gougeon et al. Human Reproduction 1986
In vitro
activation
protocols
? Controlled
Ovarian
Hyperstimulation
(COH)
-Kawamura et al. PNAS. 2013.
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•
•
•
ROBUST ENRICHMENT OF IGF-1 IN CULTURE
ng
/mL
P<0.001
Vectors-IGF-1
Human Endothelial Cells
0
200
400
600
800
1000
1200
1400
1600
ECs IGF-ECs
IGF-1
EXPERIMENTAL DESIGN
Human Ovarian Tissue
Fibrin clot
CONTROL GROUP STUDY GROUP
END POINT 3 WEEKS n=8 n=6
0
20
40
60
80
PrimordialPrimarySecondary
Blank
IGF
P=0.41 P=0.03 P=0.05
%
0
5
10
15
20
25
30
SecSimple Secpre-antral
Blank
IGF
P=0.22 P<0.001
%
CELL-BASED STRATEGY
-Gougeon et al. Human Reproduction 1986
In Vitro
Activation
Protocols
IGF-1
Co-
Transplantation COH
Daylon James
Laura Park
Jovana Lekovich
Glenn Schattman
Zev Rosenwaks
Nikica Zaninovic
Richard Bodine
Michael Ginsberg