Download - Living cerebral endothelial cells
György Váró
Institute of Biophysics, Biological Research CenterSzeged, Hungary
Lamorlaye, May 2011
2
Mannitol treatment of the cerebral endothelial cells
Blood-brain barrier:
Mannitol:
HO - CH2 – (CHOH)4 – CH2 - OH
3
Before treatment After treatment
4
Before treatment
After treatment
5
0.0
0.5
1.0
1.5
2.0
2.5
Cell region
Hei
ght
(µm
)
Control
Mannitol
Over the nucleus Out of nucleus
6
8 kPa
0.9 kPa
0
0.2
0.4
0.6
200 0-200-400-600
forc
e (n
N)
distance (nm)
SM C
A
indentation (nm)
forc
e (n
N)
0
0.2
0.4
0.6
6004002000
MC
B
7
Z. Bálint, I.a. Krizbai, I. Wilhelm, A.E. Farkas, Á. Párducz,Z. Szegletes and G. Váró (2007)
Changes induced by hyperosmotic mannitol in cerebral endothelialcells: an atomic force microscopic study.
Eur. Biophys. J. 36. 113-120
8
Effect of the calcium starvation on the cells:
Healthy endothelial cells-Ca2+
Ca starved cells
9
Ca starved cells
+Ca2+
10
I. Wilhelm, A.E. Farkas, P. Nagyőszi, G. Váró, Z. Bálint, G.A. Végh,P.O. Couraud, I.A. Romero, B. Weksler and I.A. Krizbai (2007)
Regulation of cerebral endothelial cell morphology by extracellular calcium.
Phys. Med. Biol. 52. 6261-6274
11
Petri Dish
8 µm Filter
AFM cantilever
Matrigel
RBEC LayerA2058
Transmigration of the melanoma cells
12
A2058 on RBEC layer
13
3
AFM cantilever tip on hard surface
Petri dish
AFM cantilever tip on hard surface
dwell time
load
AFM cantilever tip on living cells
AFM cantilever tip on living cells
Stress relaxation
AFM tipless cantilever with melanoma cell on living cells
AFM tipless cantilever with melanoma cell on living cells
AFM tipless cantilever with melanoma cell on living cells
AFM tipless cantilever with melanoma cell on fixed cells
Adhesion force: Melanoma cell on
hard surface
Melanoma cell onliving cell layer
Melanoma cell onfixed cell layer
Stress relaxationforce
Melanoma cell on hard surface
Melanoma cell onliving cell layer
Melanoma cell onfixed cell layer
Adhesion force properties:
Numbers of jumps
Size of the jumps
25
Living cells
Fixed cells
Size of the jumps
Stress relaxationfast exp.
Stress relaxationslow exp.
28