Human Hematopoietic Stem CellCD34CD38low/neg
CD59CD90 (Thy1)
CD117 (c-kit)CD133CD135 (Flt3)CD164
CD338linneg
GaTa2 TdT
Multi-Potent Progenitor (MPP)CD34CD135 (Flt3)
linneg
TdT
Common Lymphoid Progenitor (CLP)
Pro-BCD10CD19CD20CD22CD34neg
CD72CD74
CD124CD164CD252CD268CD275HLa-DR
Pre-NK/TCD5CD34
Pro-NKCD7CD10neg
CD34neg
CD117 (c-kit)
Immature NKCD34neg
CD94neg
CD117 (c-kit)CD122CD161Ets-1 Id2 Id3
NKTCD3CD8 CD16 CD56CD69CD161Vα24Jα18TCRId2 PLZF
CD4 ISP (Immature Single Positive)CD1aCD2CD3 CD4CD5
CD7CD34CD45ROCD127 (IL-7Rα)TCRβlow
Early Double Positive (EDP)CD1aCD2CD3 CD4 CD5CD7CD8α CD38CD45RO CD127 (IL-7Rα)TCRβ
Double Positive (DP)
γδ TCD4neg
γδ TCRSox13
CD8CD8 Granulysin Granzyme a Granzyme B Perforin Runx3
CD4CD4
Pre-BCD10CD19CD20CD22CD34neg
CD72CD74CD79a
CD124CD164CD252CD268CD275 HLa-DRIgMPax5
Immature BCD5 CD10CD19CD20 CD21 CD22CD23 CD24CD32CD37CD40CD48 CD52CD72CD75 CD79aCD79bCD80act
CD84CD86act
CD98CD124CD137 (41BB)act
CD152act
CD153CD180CD184CD185CD196CD200CD210CD229 CD252act
CD253CD267 (TaCI)CD275CD279 (PD-1)CD307CD317 (BST2)CD319 (CRaCC)IgDIgGIgM
Pre-TCD1aCD2CD3 CD5CD7high
CD28CD34CD45CD127 (IL-7Rα) NOTCH1
Mature BCD5CD19 CD20 CD21CD22CD23act
CD24CD27act
CD32CD35CD37CD40CD48act
CD49bCD49cCD49dCD52CD72CD75 CD79aCD79bCD80act
CD84CD86act
CD98CD124CD137 (41BB)act CD152 (CTLa-4)act
CD180 CD184 (CXCR4)CD185 (CXCR5)CD192 (CCR2)act
CD196 (CCR6)CD200CD210CD229CD252act
CD253act
CD267 (TaCI)CD268 (BaFFR)CD269 (BCMa)CD272CD275CD279 (PD-1)act CD307CD317 (BST2)CD319 (CRaCC)CD360 (IL-21R)HLa-DRIgDIgGIgMBlimp-1 Bcl-6 Pax5
Plasma
Common Myeloid Progenitor (CMP)CD33CD34CD45RaCD117 (c-kit)
CD123low
CD131CD164CD173
CD174Ikaros PU.1
Megakaryocyte- Erythroid Progenitor (MEP)
Granulocyte-Myeloid Progenitor (GMP)CD34 CD45RaCD123PU.1
CD33CD34CD36CD110CD123high
CD131CD164FOG-1 GaTa1 GaTa2
MacrophageCD11aCD11c CD14CD16CD19 CD23
CD26CD31CD32CD36CD40CD45RO
CD52CD63CD68 CD71CD74CD87
CD88CD92CD119CD121bCD123CD155
CD156aCD163 CD169CD170 CD195CD204
CD206CD210CD280CD282 (TLR2)CD284 (TLR4)CD312
CD354 (Trem-1)CD357 (GITR)HLa-DRTrem-2PU.1
Conventional DC PrecursorCD11cCD14neg
CD33dim
Common DC ProgenitorCD11cCD33dim
Plasmacytoid DC (pDC)
Conventional DC (cDC)
Monocyte
Mature NKCD2CD7CD8CD11bCD15s CD16CD27CD32CD43CD45RCCD49dCD56 CD57CD62L CD66aCD81CD85d (ILT4)CD85j (ILT2)CD87CD94CD96CD99RCD119CD122CD132CD158a CD158b CD158f CD159a (NKG2a)CD159c (NKG2C)
CD160CD161CD162RCD178CD181 (CXCR1)CD182 (CXCR2)CD183 (CXCR3)CD198CD210CD212CD218aCD218bCD223CD226CD244CD245CD247CD253CD277CD300aCD305CD319 (CRaCC)CD328 (Siglec-7)CD335 (NKp46)CD336CD337Granzyme a Granzyme B Perforin
Neutrophil Eosinophil
TregCD25CD39CD73CD101CD127low
CD152 (CTLa-4)CD183 (CXCR3)CD194 (CCR4)CD199 (CCR9)CD357 (GITR)GaRPact
Foxp3 Helios
Th17CD161CD121a (IL-1R1)CD196 (CCR6)CD360 (IL-21R)IL-23RBaTF IRF4 RORγt
Th1CD26CD94CD178 (FaSL)CD195CD212 (IL-12RB2)CD218 (IL-18R)CD254 (TRaNCE)CD278 (ICOS)NOTCH3Tim-3T-bet
Th2CD30CD124 (IL-4R)CD184 (CXCR4)CD198 (CCR8)IL-17RBNOTCH1NOTCH2Tim-1 GaTa3
Th9CD124 (IL-4R)IL-17RBIRF4
Th22CD140 (PDGFR)CCR10
TfhCD84CD126 (IL-6R)CD185 (CXCR5)CD278 (ICOS)CD360 (IL-21R)Bcl-6
Mast BasophilCD9CD11aCD11bCD13CD16CD17CD25CD32CD33CD38CD43CD88CD123CD125CD218 (IL-18R)CD154CD192CD193CD203c CD281 (TLR1) CD282 (TLR2)CD284 (TLR4)CD286 (TLR6)CD289 (TLR9) CD294FcεR1IgE C/EBPα GaTa2
MegakaryocyteCD41CD42aCD42bCD42cCD42dCD49f
CD51CD61CD110CD112CD123
PlateletCD9CD17CD23CD31CD36CD41CD42aCD42bCD42cCD42dCD49bCD49f
CD51CD60aCD61CD84CD92CD110CD147CD151CD173CD226GaRP
Activated PlateletsCD54CD62pCD63
CD107aCD107bCD109
ErythrocyteCD35CD44CD123CD173CD233CD234CD235aCD235b
CD236CD236RCD238CD239CD240CD241CD242
General T Cell MarkersCD2CD3 CD5CD6CD7CD27CD28CD30
CD35 CD40 CD43CD45Ra CD45RB CD45RC CD52CD57
CD60aCD60bCD60c CD62L CD75sCD81CD84 CD85j (ILT2)
CD89 CD94 CD98CD99RCD101 CD103 CD107aact
CD107bact
CD119CD121bCD122CD123 CD127CD130CD132CD150
CD158a CD158b CD158f CD159a CD160 CD161 CD168CD171
CD172a (SIRPα) CD181 (CXCR1) CD182 (CXCR2) CD184 (CXCR4) CD191 (CCR1)CD192 (CCR2) CD193 (CCR3) CD195 (CCR5)
CD196 (CCR6) CD197 (CCR7) CD198 (CCR8) CD199 (CCR9) CD210CDv218aCD218bCD226
CD229CD244 CD247CD277CD279 CD300a CD300c CD305
CD316CD317 (BST2)CD319 (CRaCC)αβ TCR
CD4low
CD9CD11bCD11cCD13CD14 CD15CD17CD28CD31CD32CD33CD35CD36CD38CD40CD45ROCD45RaCD45RBCD49bCD49eCD49fCD63CD64CD65sCD68CD74CD84
CD85a (ILT5)CD85d (ILT4)CD85h (ILT1)CD85j (ILT2)CD85k (ILT3)CD86CD87CD89CD91CD92CD93CD101CD111CD112CD115CD116CD119CD121bCD123CD131CD142CD147CD155CD156aCD157CD162CD163CD168CD170
CD171CD172a (SIRPα)CD172bCD180CD184 (CXCR4)CD191 (CCR1)CD192 (CCR2)CD195 (CCR5)CD198 (CCR8)CD210 (IL-10R)CD213a1 (IL-13Rα1)CD213a2 (IL-13Rα2)CD226CD277CD281 (TLR1) CD282 (TLR2)CD284 (TLR4)CD300aCD300cCD300e (IREM-2)CD300f (IREM-1)CD302CD305CD312CD317 (BST2)CD322CD329GITRLPU.1
CD10CD15CD16bCD17CD24CD32CD35CD43CD66aCD66bCD66dCD89CD93CD112CD114CD116CD157CD177CD181 (CXCR1)CD281 (TLR1) CD282 (TLR2)CD284 (TLR4)CD286 (TLR6)CD289 (TLR9) MPO Trem-1
CD1cCD1dCD2CD4low
CD11b CD11c CD13CD14neg
CD33CD45ROCD64 CD68CD80CD85j (ILT2)CD85k (ILT3)CD86CD105CD112CD123neg
CD141low CD163 CD172a (SIRPα) CD195 (CCR5)CD197 (CCR7)CD205 CD282 (TLR2)CD283 (TLR3) CD284 (TLR4)CD286 (TLR6)CD300eCLEC-9aHLa-DR
CD4CD11cneg
CD45RaCD49dCD85g (ILT7) CD85k (ILT3)CD123 CD282 (TLR2)neg
CD284 (TLR4)neg
CD289 (TLR9) CD303 (BDCa-2) CD304 (BDCa-4) HLa-DRTLR7 Spi-B
CD9CD11bCD15CD24CD32CD35CD43CD64CD116CD117 (c-kit) CD123CD125CD126CD181 (CXCR1)CD244FcεR1 IgE
CD9CD11bCD15CD24CD32CD35CD64CD244CD116CD123CD125CD126FcεR1IgE
–– = Proposed Pathway
act = Expressed On activated
low = Low Expresion
high = High Expresion
neg = Negative
= Intracellular antigen
= Subset
= Pan Marker/Common
Defining Marker
LEGEND
LYMPHOID MYELOID
CD117 (c-kit)low
CD127CD135 (Flt3)CD164HLa-DRaiolos c-mybhigh
C/EBPαneg GaTa2neg GaTa3 PU.1high TdT
CD7CD10CD24neg
CD34CD38CD45RaCD90low
CD133CD138 CD183 (CXCR3) CD184 (CXCR4) CD269 (BCMa)aiolos Blimp-1 IRF4 XBP-1
CD19neg
CD20neg
CD27CD252CD52neg
CD126 CD38CD72neg
CD79a
CD1aCD2CD3 CD4CD5CD7CD8α
CD8βCD45ROCD127 (IL-7Rα)TCRβ ThPOK TOX
Differentiation of the Human Hematopoietic Stem Cell
Human hematopoietic development from the hematopoietic stem cell to the end stage cells illustrating markers found at each stage as determined through the 9th HLDA congress.
Q312061 Hematopoietic Stem Cells 0912
www.eBioscience.com
©2012 eBioscience, Inc. An Affymetrix Company. All rights reserved.
Customers in countries where direct sales are not available may contact their eBioscience distributor listed at www.eBioscience.com/distributors
Differentiation of the Human Hematopoietic Stem CellNORTH AMERICA: CUSTOMER SERVICE 888.999.1371 • [email protected] | TECHNICAL SUPPORT 888.810.6168 • [email protected]
EUROPE: CUSTOMER SERVICE +43 1 796 40 40-304 • [email protected] | TECHNICAL SUPPORT +43 1 796 40 40-120 • [email protected]
