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Ming Yan
Purification and Reengineering of Plastic-Degrading
Enzymes
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Plastic bags are made from polyethylene (PE), a polymer consisting of long chains of the ethylene monomer (C2H4).
Species of bacteria belonging to the Streptomyces genus are capable of producing polyethylene-degrading enzymes.
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Streptomyces
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Streptomyces
Streptomyces viridosporus
Streptomyces badius
Streptomyces setonii
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How is Biodegradability Measured?
Weight lossTensile strength loss
measured stress at fracture of the specimen
Changes in percent elongation extension of material under load
Changes in polyethylene molecular weight distribution polydispersity
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Project Ideas
Aim 1: To purify and characterize the biodegradable polyethylene-degrading enzyme(s) from bacterium belonging to the Streptomyces species
Aim 2: To enhance the biodegradability of the polyethylene by increasing the efficiency of the degrading enzymes through protein engineering techniques
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Aim 1
Inspiration Enzymes identified for anaerobic degradation of
polyethylene glycol (PEG)
Technical Difficulties with the isolation methods
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Aim 2
To enhance the biodegradability of the polyethylene by increasing the efficiency of the degrading enzymes through protein engineering techniques
Methods: Score system Protein engineering
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Protein-Engineering
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More Ideas