Download - Neuron cell under strong laser beam
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Neuron cell under strong laser beam
江宏仁 2003.07.07
Living soft matter ?
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NEURONAL MORPHOGENESIS ANDSTRUCTURAL PLASTICITY:
Axon Initiation Axon GrowthAxon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid MotilitySynapse Formation and Stability Axon and Dendrite Retraction
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PHENOMENOLOGY
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Growrh cone
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protrusion
anchorage
forward movement
tail retraction
4 processes coordinated:
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Axon Guidance
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Laser:1064nm 700mW source 50 mW after nosepiece
Guiding neuronal growth with light ?
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Optical forces can impact diffusive behavior.
Theory 1
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Mechanical manipulation of a membrane.
Theory 2
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Dynamic Excitations in Membranes Induced by Optical Tweezers
Colloidal effects
Theory 3
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PEARLING OF CYLINDRICAL VESICLES
Laser-induced rounding
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Tests for each theory
Theory 1 :* in vitro actin polymeried under laser beam * Listeria movement as membrance moving model
Theory 2: *using flow cell to apply shear force on membrance
Theory 3: *using indirect phenomena ex: pearling, rounding, retrcation, or in water *direct observe by EM or light scattering