![Page 1: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/1.jpg)
New Methodology
We anticipate that BFresh will become the gold standard in food
industry and is likely to eradicate food
shortage.
Usages Unlimited
Our innovative product will shield food from microorganisms and
pests.
Increase Your Sales
Offering an organically preserved product to your customers will
dramatically increase your revenue.
Global Impact
Our product will affect food preservation as we
know it, on a grand global scale.
![Page 2: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/2.jpg)
Testing BFresh
Non-preserved, Day 8 BFresh, Day 8
Non preserved, Day 0 BFresh, Day 0
![Page 3: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/3.jpg)
The Kit Components
When you sign MTA with BFresh, Inc., you will receive a BFresh test kit, which includes all the components for testing antimicrobial properties of BFresh formulation on bacteria and fungi grown over Agar plates as well as on fruits or other types of food
This is a blind test so that the identity of each membrane (A, or B) is not known to you at the time of testing. Before the test starts, you will receive a password protected PDF file. You will receive the password for opening the PDF file after you return all the kit components.
BFresh test kit, includes the components shown here
BFresh membrane holder
![Page 4: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/4.jpg)
You will receive two Eppendorf tubes with sufficient concentration of mixed salivary bacteria and mixture of fungi from pineapple, strawberry, raspberry, banana, bread and tomatoe for overnight growth.
Bacteria
Fu
ng
i
Eppendorf tubes with bacteria
and fungi
BFresh test kit, includes the components shown here
The Kit Components
![Page 5: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/5.jpg)
You will also receive two sets of Agar plates, and a spreader for spreading the bacteria or fungi over the agar plates.
Agar plates
For bacterial growth
For fungal growth
Spreader
BFresh test kit, includes the components shown here
The Kit Components
![Page 6: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/6.jpg)
You will receive multiple cassettes containing either the control or BFresh membranes, and a sprayer for wetting the membranes.
BFresh membrane
BFresh membrane holder
Sprayer
BFresh test kit, includes the components shown here
The Kit Components
![Page 7: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/7.jpg)
You will receive an incubation and observation chamber for viewing the growth of microorganisms over the agar plates or on food.
Incubation and observation chamber
BFresh test kit, includes the components shown here
The Kit Components
![Page 8: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/8.jpg)
Open the packet that includes the spreader. Two spreaders are provided one for spreading bacteria and the other for spreading fungi over agar plates.
The Kit Components
Spreader
Carry out the test for bacteria and fungal cultures as shown below.
![Page 9: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/9.jpg)
Open Eppendorf tube containing bacteria, hold the spreader with the L shaper part and insert the tip of spreader into the tube. Remove the spreader from the tube.
The Kit Components
Carry out the test for bacteria and fungal cultures as shown below.
![Page 10: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/10.jpg)
Touch the top of the agar plate with the tip of the spreader and then in a zigzag motion, gently move the tip of spreader over the agar surface. Repeat this process for spreading fungi over a separate agar plate.
The Kit Components
Carry out the test for bacteria and fungal cultures as shown below.
![Page 11: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/11.jpg)
Remove the top glass cover of each incubation/observation chamber.
The Kit Components
Carry out the test for bacteria and fungal cultures as shown below.
A
![Page 12: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/12.jpg)
Place one set of agar plates streaked with bacteria and fungi within chamber A and one set in chamber B. Within each chamber there is a membrane labeled A or B.
The Kit Components
Chamber A includes bacterial
and fungal cultures and membrane A
Chamber B includes bacterial
and fungal cultures and membrane B
Carry out the test for bacteria and fungal cultures as shown below.
A B
![Page 13: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/13.jpg)
The Kit Components
Carry out the test for bacteria and fungal cultures as shown below.
Wet the membranes with the sprayer and then invert each glass top over the bottom plastic cover. Apply pressure over each glass until it snaps closed.
Chamber A includes bacterial
and fungal cultures and membrane A
Chamber B
Chamber B includes bacterial
and fungal cultures and membrane B
Chamber A
A B
![Page 14: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/14.jpg)
Keep chambers at room temperature and observe each chamber over several days. You will see bacterial and fungal growth in one of the two sets A or B.
The Kit Components
Carry out the test for bacteria and fungal cultures as shown below.
Chamber A includes bacterial
and fungal cultures and membrane A
Chamber B
Chamber B includes bacterial
and fungal cultures and membrane B
Chamber A
A B
![Page 15: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/15.jpg)
The bacterial and fungal colonies appear over the surface of agar in about 1-2 days and growth increases further over time.
Bacteria
Fungi
No Growth
Carry out the test for bacteria and fungal cultures as shown below.
The Kit Components
![Page 16: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/16.jpg)
Test fruit spoilage in chambers C-D as shown below
The Kit Components
Raspberry is the best fruit choice for testing since it allows you to see the fungal growth over the fruit in 1-2 days. However, you can place strawberry, blackberry or bread within the chamber. Fungal growth is readily visible through the glass top.
C
![Page 17: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/17.jpg)
Carry out the test on fruits as shown below.
The Kit Components
In two separate dishes, labeled C and D, place fruits over the bottom plastic covers.
C
![Page 18: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/18.jpg)
Carry out the tests on fruits as shown below.
The Kit Components
Wet the membranes with the sprayer and then invert each glass top over the bottom plastic cover. Apply pressure over each glass until it snaps closed.
Chambers C&D
C D
![Page 19: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/19.jpg)
Carry out the test on fruits as shown below.
The Kit Components
View the fruits/food through the glass to see the growth of fungi on their surfaces
![Page 20: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/20.jpg)
Return the kit components
Record the results of the tests and then mail the kit components to the following address: 16471 Scientific Way, Irvine CA 92618
The Kit Components
![Page 21: New Methodology We anticipate that BFresh will become the gold standard in food industry and is likely to eradicate food shortage. Usages Unlimited Our](https://reader035.vdocument.in/reader035/viewer/2022062619/551638dd550346a2308b633c/html5/thumbnails/21.jpg)
Thank you for your interest in BFresh, Inc
Do not let a great taste go to waste