Transcript
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NGS needs for study capture
Frans P Ruzius
Hubrecht Institute
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NGS
• Next Gen(eragion) Sequencing
• Deep Sequencing
– Small reads
• 50-500 bp
– Lots of reads
• 0.5 – 800 M
– Big reference
• Genome
– 3 Gb
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Sequencing run
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Experiment webpage
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Sequencing run
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Experiment webpage
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Sequencing run
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Mapping pipeline
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Sequencing run – future planns
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Submission website
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Sequencing run
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Need for sample tracking
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Most heard questions in the lab
• Can this be solved by study capture?
– Is my data already analyzed?• What is already mapped and what not?
– Where can I find my data?• Where is the data stored?
– From which run was my library?
– What was the mappability?