Supplemental Figure 3. Cross-correlation analyses of clock gene expression in human peripheral blood leukocytes challenged with endotoxin or saline at 09:00 h or 21:00 h. The gene expression data for each subject was cross-correlated across all studied genes within a subject. The cross-correlation data were normalized to one, where one represents a perfect correlation between gene responses. The correlation matrices for (A) placebo day and night subjects (09:00 h plus 21:00 h saline infusion time; n=3), (B) endotoxin day subjects (09:00 h infusion; n=4), (C) endotoxin night subjects (21:00 h infusion; n=3), (D) endotoxin day and night subjects (09:00 h plus 21:00 h endotoxin infusion time; n=7), were clustered into a tree form.

Per2

Bmal1

Per3

Cry2

Cry1

Clock

Ror

CSNK1

Per1

Rev-erb

0

0.05 0.

1

0.15 0.

2

0.25 0.

3

0.350

0.05 0.

1

0.15 0.

2

0.25 0.

3

0.35

Per2

Bmal1

Per3

Cry2

Cry1

Clock

Ror

CSNK1

Per1

Rev-erb

Per2

Bmal1

Per3

Cry2

Cry1

Clock

Ror

CSNK1

Per1

Rev-erb

A B

D

Per2

Bmal1

Per3

Cry2

Cry1

Clock

Ror

CSNK1

Per1

Rev-erb

0

0.05 0.

1

0.15 0.

2

0.25 0.

3

0.35

C

0

0.05 0.

1

0.15 0.

2

0.25 0.

3

0.35