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![Page 1: Physical Cell Biology - ManningGroup · 2018-10-11 · Figure 8.3 Physical Biology of the Cell (O Garland Science 2009) 1, nr— 3 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 -40 -20](https://reader035.vdocument.in/reader035/viewer/2022081407/5f2b1663c86692574115db0c/html5/thumbnails/1.jpg)
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Physical Cell BiologyLecture 14:DNA and random walks
DNA: Phillips, Chapter 8,, Pollard chapter 7-9
Levels of AbstractionMicrotubuleDNA
Polyethylene
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Molecules as chains of small units
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Polymer configuration as a random walk
Distribution of End to End Distances
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Radii of Gyration for Different Chromosomes
Persistence Length
Figure from : Liverpool, T.B., “Active gels: where polymer physics meets cytoskeletal dynamics” Philosophical Transactions of the Royal Society – A, 364(1849), 2006, p. 3335-3355
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DNA and persistence length scales
Single Molecule Mechanics
AFM
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Force extension experiments
Optical trap/tweezers
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Magnetic tweezers
Pipette based force measurements
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