THE ROAD TO OUR SOLUTION
TUMOR IMAGING
MRI scan of latter stages of brain cancer
Photograph by Zephyr/Science Photo Library
2007 National Geographic Society
TEAM TU-EINDHOVEN
The Sensor
The Chassis
Contrast
Agents
Safety
Detect the hypoxic environment
Generate contrast on an MRI scanner
Integrate the sensor and contrast agents
Ensure patient safety
TEAM TU-EINDHOVEN
The Sensor
Safety The Chassis Contrast
Agents The Sensor
Shift to low oxygen concentration
INTENSITY
INCREASES OVER
TIME
TEAM TU-EINDHOVEN
The Sensor
Safety The Chassis Contrast
Agents The Sensor
INTENSITY
INCREASES OVER
TIME
TEAM TU-EINDHOVEN
The Sensor
Safety The Chassis Contrast
Agents The Sensor
• High Lysine Ratio
TEAM TU-EINDHOVEN
Contrast Agents
Define
chassis Safety The Chassis
Contrast
Agents The Sensor
1PJN
• Practicality
• Molecular
Dynamics
Simulations
TEAM TU-EINDHOVEN
// 1ETF
// 1G70
// 1PJN
// Human Protamine 1
// Poly Arginine-Glycine
// Poly Arginine-Serine
// Poly Threonine-Lysine
// Poly Lysine-Serine
Define
chassis Safety The Chassis
Contrast
Agents The Sensor
THE SELECTION
Contrast Agents
AEROBIC EXPRESSION
NATIVE PROTEINS ARE CLEARLY EXPRESSED
TEAM TU-EINDHOVEN
Contrast Agents
Define
chassis Safety The Chassis
Contrast
Agents The Sensor
THE BIOBRICKS
TEAM TU-EINDHOVEN
The Chassis
19 NEW BIOBRICKS:
- A FUNCTIONAL ANAEROBIC PROMOTER
(BBa_K1123000)
- 9 CEST PRODUCING PROTEINS
(BBa_K1123013 – BBa_K1123021)
- 9 COMPLETE TUMOR VISUALIZATION CONSTRUCTS
(BBa_K1123001 – BBa_K1123009)
Safety The Chassis The Sensor Contrast
Agents
The Safety Locks
TEAM TU-EINDHOVEN
Define
chassis Safety The Chassis The Sensor
Contrast
Agents
AVOIDING AN IMMUNE
SYSTEM RESPONSE
Outer
Membrane
Periplas
m
Cytoplas
mic
Membrane
Illustration by David S. Goodsell, the
Scripps Research Institute.
The Safety Locks
TEAM TU-EINDHOVEN
THE KILL SWITCH
Define
chassis Safety The Chassis The Sensor
Contrast
Agents
Herpes Simplex Virus Thymidine Kinase- Prodrug Ganciclovir System
TEAM TU-EINDHOVEN
THE KILL SWITCH MODEL
Define
chassis Safety The Chassis The Sensor
Contrast
Agents
The Safety Locks
// Bringing the world of synthetic biology
into everybody’s home.
// Removing the reservations people
have about synthetic biology by
removing faulty data.
// Collaborating and collecting data
across the globe.
TEAM TU-EINDHOVEN
SYNTHETIC FACTS
Human Practices
SYNTHETIC FACTS
21 Initial Facts To Be Checked.
12 Main Topics.
10 Facts Are Officially Checked, Uploaded And Available.
TEAM TU-EINDHOVEN
Human Practices
TEAM TU-EINDHOVEN
We have established
the foundations for a
new bacterial
application.
“Synthetic Facts” will
continue to give
reliable and unbiased
information.
TEAM PICTURE!!! Special Thanks To… // dr. Maarten Merkx
// dr. ir. Tom de Greef
// dr. ir. Natal van Riel
// ir. Stijn Aper
// Sagitta Peters MSc.
// Industrial Designer Mariana Felix
// ir. Stephanie Hectors
// prof.dr. Klaas Nicolaij
// dr. ir. Koen Pieterse
TEAM TU-EINDHOVEN
Acknowledgements
The Sensor
TEAM TU-EINDHOVEN
Global design of the FNR-single and FNR-tandem promoter
Reactions of the FNR-tandem
promoter
Safety The Chassis Contrast
Agents The Sensor
CEST PRINCIPLES
Contrast Agents
TEAM TU-EINDHOVEN
Define
chassis Safety The Chassis
Contrast
Agents The Sensor
Tumor
Targeting
Kill Switch
Environmental
Sensor
Detectable by
CEST MRI
Escherichia Coli
The Chassis
TEAM TU-EINDHOVEN
Define
chassis Safety The Chassis
Contrast
Agents The Sensor
The Safety Locks
TEAM TU-EINDHOVEN
Define
chassis Safety The Chassis The Sensor
Contrast
Agents
AVOIDING AN IMMUNE
SYSTEM RESPONSE
Rough
Cytoplasm
Journal of Endotoxin Research, Vol.
7, No. 3 , 2001
Polysacchar
ide Surface
Layer
Outer
Membran
e
Cytoplasm
ic
Membrane
O-Antigen of LPS
K Capsule
Lipid A of
Lipopolysacch
aride (LPS)
Peripla
sm
Smooth
Rough
THE KILL SWITCH MODEL
TEAM TU-EINDHOVEN
Define
chassis Safety The Chassis The Sensor
Contrast
Agents
The Safety Locks
THE ALTERNATIVE
KILL SWITCH
TEAM TU-EINDHOVEN
The Safety Locks
Define
chassis Safety The Chassis The Sensor
Contrast
Agents