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Capillary zone electrophoresisand affinity capillary electrophoresis
simulator
Step-by-step User Guide
PeakMaster 6
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PeakMaster 6 - User Guide
Input Data
Complexation
Results
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Input Data
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Input Data
Add the components of your system(BGE component or analyte).
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Input Data
Add the components of your system(BGE component or analyte).
Type of the component:NUCLEUS or LIGAND
This is explained in detail in theComplexation part.
In this example, we will add all thecomponents as ligands and then theselector (β-cyclodextrin) as nucleus.
You can skip this step unless you want to work with complexing
systems.
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Input Data
Add the components of your system(BGE component or analyte).
Pick a component fromthe database.
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Input Data
Add the components of your system(BGE component or analyte).
Start typing ...
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Input Data
Add the components of your system(BGE component or analyte).
Pick the desiredcomponent.
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Input Data
Add the components of your system(BGE component or analyte).
Confirm.
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Input Data
Add the components of your system(BGE component or analyte).
If you cannot find the component in the database ...
Lithium added.
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Input Data
Add the components of your system(BGE component or analyte).
Type in the name.
Then add the respectiveionic form
(-1 for anionic, 1 for cationic).
Choose correct type.
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Input Data
Add the components of your system(BGE component or analyte).
Enter the physico-chemical parameters of
the ionic form
Electrophoretic mobility
pKa
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Input Data
Add the components of your system(BGE component or analyte).
Now add the selector –type nucleus.
If you do NOT deal withcomplexation, you can ignore the
selector.
CHES added.
This part is relevant only for complexing systems.
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Input Data
Add the components of your system(BGE component or analyte).
Name and type.
If the compound isneutral, you do not needto enter any ionic form.
This part is relevant only for complexing systems.
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Input Data
Add the components of your system(BGE component or analyte).
Add the analyte(s) in the same way as theBGE components.
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Input Data
Add the components of your system(BGE component or analyte).
Zero concentration:PM 6 cannot operate with zero
concentrations.
If you enter concentration whose value is lower than the allowed limit, it will be changed automatically to the lowest
acceptable concentration.
After adding all the components of your system, enter their concentrations (may differ in the BGE
zone and in the sample zone).
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Input Data
Add the components of your system(BGE component or analyte).
Double-click on the Name or Type of theconstituent to edit the parameters.
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Input Data
Add the components of your system(BGE component or analyte).
When the system is complete, let PeakMaster calculate its parameters.
Click the Calculate! button or hit F5.
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Input Data
Add the components of your system(BGE component or analyte).PeakMaster 6 has calculated the system parameters
and shows the conductivity electropherogram.
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Input Data
Add the components of your system(BGE component or analyte).
Nonideality corrections(ionic effects, viscosity)
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Input Data
Add the components of your system(BGE component or analyte).
Correction for ionic effects(Debye-Hückel and Onsager-
Fuoss) enabled by deafult
Correction for viscosityis considered experimental
disabled by default
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Input Data
Add the components of your system(BGE component or analyte).
Editing the database.
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Input Data
Add the components of your system(BGE component or analyte).You can edit the existing
database …
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Input Data
Add the components of your system(BGE component or analyte).… or load a completely
different one.
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Input Data
Automatic pH adjustment(since PM6.0f8)
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Input Data
Pick a constituentwhose concentraionyou want to adjust to achieve the target pH
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Input Data
1. Enter thedesired pH value
2. Click Adjust
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Input Data
Check the adjustedconcentration of the
constituent
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Input Data
Keep in mind that in may not be always possible to achieve thedesired pH by adjusting the concentration of the selected
constituent. If that happens, you will receive an error message.
Additionally, automatic pH adjuster may not work reliably withhighly concentrated solutions (IS of 1000mM or above).
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Complexation
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Complexation
There are few basic rules for describing complexation in the PeakMaster 6.
• NUCLEUS – any ionic form of a nucleus can interact with an arbitrary numberof ionic forms of ligands– each complex MUST contain ONE AND ONLY ONE nucleus
• LIGAND – any ionic form of a ligand can interact with any ionic form of nuclei
• Ligands can NOT interact with other ligands and the same goes for nuclei.
• A FEW EXAMPLES:(N – nucleus, L – ligand, K – ligand)
Allowed
• NL2
• NL3K• NLK4
! Not allowed
• N2L• N4K• LK
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Complexation can be established by dragging the compound and dropping it on the other one.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Assisted ligand – nucleus conversion:In case you forget to change the type …
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Assisted ligand – nucleus conversion:PeakMaster will ask you which of them
do you wish to convert!
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Enter the complexationparameters for each ionic form.
Complexation constant (Kxs) isrelated to SI units (M-1) .
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Complexation between the two compounds established.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Adding second complexation with the BGE component (drag and drop again).
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Enter the parameters of thesecond complexation.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
New complexation established.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
New selector (nucleus type) added –establishing new complexation (drag and drop).
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
Enter the parameters of thiscomplexation.
Note that if you leave therow empty, it means the
respective ionic form doesnot form a complex.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
New complexation with a different nucleus established.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
To edit the complexation, double clickon the nucleus coloured field.
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Complexation
Fill in the CommonParameters (software
keeps the values until youchange them).
You can choose whichligand interaction you
want to edit.
By removing the ligand you can remove the
complexation.
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Results
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Results
System properties.
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Results
Eigenzone properties.
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Results
Parameters marked as Max presentthe values in peak apex
(not the same as the values in Eigenzone details).
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Results
Zone composition blockdisplayed in RED indicatesthat PeakMaster failed to
calculate composition of thatzone accurately. Keep that in
mind when you use such results.
This inaccuracy does notaffect mobilities and
migration times.
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Results
Check if you want to display the results as a differencebetween the zone and the
pure BGE parameters.
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Results
Change ofconcentration of thechosen component in the respective zones.
Conductivity change in the respective zones.
Mobilities of systemand analyte peaks.
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Results
Ionic composition.
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Results
BGE tab shows ioniccomposition of the BGE
at the given pH.
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Results
In the Analytes tab you can see degreeof dissociation of the chosen analyte.
If there is a warningpresent, the
dissociation states maynot be correct!
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Results
Predicted electropherogram.
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Results
You can change the initialinjection zone length.
Replot to see the change.
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Results
Changed injection zone length.
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Results
Choose which profile you wantto display
(conductivity, pH, concentration)
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Results
CHES concentration profile displayed.
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Results
Export the electropherogram.
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Results
Export graph as *.csv.
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Results
You can also save the wholePeakMaster setup and load it
later.
(Setup saves as *.json file.)