ProTyr Phe

Anode (attracts (-) amino acids)

Cathode (attracts (+) amino acids)

Arg

Asp

Glu

Gly Ala

Trp

Thr

Ser

Leu,Ile

MetVal

More nonpolar amino acids, interact more with the mobile phase and move more.

ProTyr Phe

Anode (attracts (-) amino acids)

Cathode (attracts (+) amino acids)

Arg

Asp

Glu

Gly Ala

Trp

Thr

Ser

Leu,Ile

Met

ValMore nonpolar amino acids, interact more with the mobile phase and move more.

* As per solutions manual

Overall (-) Overall neutral Overall (+) Polar Nonpolar

Asp Ala Arg Arg Ala

Glu Gly Asp Gly

Ile Glu Ile

Leu Leu

Met Met

Ser Ser

Thr Thr

Tyr Tyr

Val Val