Download - PULSENET AMERICA LATINA 5 ta REUNION ANUAL 23 - 25 de octubre 2007 Buenos Aires, Argentina
PULSENET AMERICA LATINAPULSENET AMERICA LATINA55tata REUNION ANUAL REUNION ANUAL
23 - 25 de octubre 200723 - 25 de octubre 2007 Buenos Aires, Argentina Buenos Aires, Argentina
PFGE-PulseNet experience – PFGE-PulseNet experience – 2006/20072006/2007
Activity plan – 2007/2008 Activity plan – 2007/2008
São Paulo State, BrazilSão Paulo State, Brazil
Ana Terezinha Tavechio
Instituto Adolfo LutzSão Paulo, Brazil
EXPERIENCE 2006-2007
CHEF DR III acquisition
BioNumerics acquisition
Salmonella Gel analysis certification
Outbreaks PFGE analysis
Serotype Total Number of Total Number of OutbreaksOutbreaks
PFGE - Number of PFGE - Number of outbreaks / isolates outbreaks / isolates
S. Enteritidis 3333 6 / 146 / 14
S. Javiana 11 1 / 21 / 2
S. Typhi 11 1 / 51 / 5
S. Typhimurium 11 1 / 71 / 7
S. Infantis 11 1 / 61 / 6
S. I 1,9,12:-:- * 11 1 / 61 / 6
Salmonella/Shigella Outbreaks analyzed by PFGE (PulseNet) – 2006-2007
Shigella sonnei 1 1 / 9
* August 2007
Salmonella Outbreaks in São Paulo State - Brazil
Lanes 1, 8, 15, Braenderup H9812; Lanes 2-7, Infantis (2007);
Lanes 9-11, Bareilly (2005); Lanes 12-14, Corvallis (2004)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
1135
668,9
452,7 398,4
336,5 310,1
244,4 216,9
138,9
104,5 78,2
54,7 33,3 28,8 20,5
Salmonella Outbreaks in São Paulo State - Brazil
Lanes 1,8,15, Braenderup H9812; Lanes 2-4, Typhimurium; Lanes 5-7, Typhimurium;
Lanes 9-10, Javiana; Lanes 11-12, Typhi * (2007); Lanes 13-14, Tennessee * (foodstuff)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
* Esporadic cases
1135
668,9
452,7 398,4
336,5 310,1
244,4 216,9
138,9
104,5 78,2
54,7
33,3 28,8 20,5
Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Lanes 1, 8, 15, Braenderup H9812; Lanes 2-4 and 5-7, S. Enteritidis (2007);
Lanes 9-11 and 12-14*, S. Enteritidis (2006)
* cake
1135
668,9
452,7 398,4
336,5 310,1 244,4 216,9
138,9
104,5
78,2
54,7 33,3 28,8 20,5
Salmonella Outbreaks in São Paulo State - Brazil
1 2 3 4 5 6 7 8 9 10
Lanes 1, 10, Braenderup H9812; Lanes 2-6, Typhi (outbreak)
Lanes 7-9, Typhi (sporadic cases) (2006)
1135
668,9
452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5
78,2 54,7 33,3 28,8 20,5
KB
Salmonella Outbreaks in São Paulo State - Brazil
Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7,8, S. enterica 9,12:-:- (August 2007);
Lane 9, S. enterica 1,9,12:-:- (esporadic case – September 2007)
1135
668,9
452,7 398,4
336,5 310,1
244,4 216,9
138,9
104,5 78,2
54,7 33,3 28,8 20,5
1 2 3 4 5 6 7 8 9 10
Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7-9, Shigella sonnei (2007)* Lane 8, different PFGE and resistance profiles
Shigella Outbreak in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10
1135
668,9
452,7 398,4
336,5 310,1
244,4 216,9
138,9
104,5 78,2
54,7
33,3 28,8 20,5
2007 - 2008 Activity Plan
PFGE analysis
Outbreaks
Extraintestinal Sporadic cases
unusual serotypes
Salmonella Gel analysis
PulseNet database of Adolfo Lutz - SP
Salmonella
E. coli O157:H7 animal reservoirs
Participation on the PulseNet Projects
2007 - 2008 Activity Plan
Ana Terezinha TavechioInstituto Adolfo LutzAv. Dr. Arnaldo, 351 – 9º andarCerqueira Cesar – São Paulo – SPCEP 01246.902 – Brasil
[email protected]@hotmail.com
THANK YOU!