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RAPID DNA:PILOT STUDY FOR LAW ENFORCEMENT
Michael P. Garvey, Jr, PhD
Director, Office of Forensic Science
Philadelphia Police Department
Stephanie Stoiloff
Senior Police Commander, Forensic Services Bureau
Miami-Dade Police Department
Jan Girten
Director, Forensic Services
Colorado Bureau of Investigation
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Rapid DNA Automated DNA process Fieldable platform Non-expert user 90-120 minute swab to answer Reference or Evidence
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VS
Is it us against them?
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We need to start somewhere…
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Law Enforcement Organizations
Philadelphia Police
Colorado Bureau of Investigation
Miami Dade Police
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Rapid DNA Vendors
GE DNAscan™ Rapid DNA Analysis™ System
IntegenX® RapidHIT® Human DNA Identification System
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Validation & Pilot Studies
Internal Validation (100 items)
Sensitivity Repeatability Reproducibility Controls Precision Mixture Mock Evidence
Reference Limited Forensic
Operational Pilots Reference samples
Lab based Field based General purpose vs. targeted
collections Arrestee collections
Limited forensic evidence Confirmatory testing Sample size Intelligence value
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Goals Law Enforcement and Forensic evaluation of current
state of technologies through two independent assessments involving multiple labs
Data shared with LE and Forensic communities to allow informed decisions
Cost comparisons Operational scenarios Development of LE and forensic protocols Work with other labs who are participating in efforts
to complete testing for NDIS approvals, etc.
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Current Status Philadelphia Police Department
DNAscan (GE Healthcare) Complete: delivered, installed, training, internal validation Pending: evaluation of forensic cartridges and operational pilot program
RapidHIT (IntegenX) Complete: delivered, installed, training In progress: internal validation of reference and forensic samples Pending: Operational pilot program
Miami Metro Dade Police Pending delivery and installation
Colorado Bureau of Investigation Pending delivery and installation
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The purpose of these studies is to provide an evaluation of each of the leading vendors of Rapid DNA.
Each study/pilot is an independent assessment. It has not been designed or performed as a head to head comparison. Any comparisons will be left to the informed consumer.
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Philadelphia Office of Forensic Science DNAscan Evaluation
April 2015
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Internal validation summary of samples tested
Promega PowerPlex™ 19 runs, 95 samples from 20 known donors
60 single source samples Sensitivity Reproducibility Repeatability NIST traceable
25 mixture samples 10 blanks
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DNAscan™ :References DNAscan RFID swab used for all samples 60 reference buccal swabs from 20 known donors
55 generated a full 16 locus profile without human intervention. 4 generated a 16 locus profile with minimal human intervention
Human intervention was limited to exporting data then importing into GeneMapper® ID-X v1.2 software for analysis.
1 sample generated partial profile at (1 swipe) Mixtures
1:1 -- all flagged as mixtures 5:1 -- 1 sample in each mixture set flagged as mixture, others only major 19:1 – all called as major contributor only
Controls All as expected
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6 swipes
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3 Swipes
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1 swipe
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DNAscan™ : Forensic Pending
Evaluation of the forensic (low concentration) cartridge
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Philadelphia Office of Forensic Science RapidHIT Evaluation
April 2015
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Test Plan and Success RatesSample Type DescriptionRapidHit # of Samples
RapidHIT % loci generated
Positive Control NIST traceable swabs 2 100
Negative Control Blank wells 7 0
Sensitivity Cotton swabs - 10, 5, 2, 1 swipes; 1 touch 10 99.1
Precision Allelic ladders 17 ±0.05
bp std. dev. Accuracy and Repeatibility Bode Buccal Collector punches (6 mm) 6 97.1
Cotton Swabs 9 99.5
Blood punches (6 mm) 4 70
Blood on swab (5 ul) 2 100
Mixtures19:1, 9:1, 5:1, 1:1, 1:5, 1:9, 1:19 (total vol 5 ul blood) 7 NA
Semen Neat semen on swab (1 ul) 1 100
Cigarette Butt Cutting 1 72
Water Bottle Cotton Swab 1 95.8
Blood on Denim Cutting on Denim (5 ul) 1 100
Preliminary Data: Validation still in progress
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5uL Blood on Denim (direct insertion)
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1uL Semen on Swab
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Cigarette Butt Filter Paper (direct insertion)
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Plastic Water Bottle (swabbed)
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Future Complete the studies Present to LE and Forensics Develop recommendations, training, and
protocols Cost analysis Technical analysis Operational analysis
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