![Page 1: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/1.jpg)
Steroids
• Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian sex hormones
• Different activities of compounds with common structural skeleton is attributed partially to:
1) Functional gps attached to steroidal nucleus 2) Overall shape conformation (dictated by
stereochemistry)
![Page 2: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/2.jpg)
SteroidsModified triterpenoids Tetracyclic of lanosterols lacking 3 CH3 gps @ C4 & C14Cholesterol fundamental structure , modifications of the side chain produce large no. of compTwo important groups of naturally occurring steroids: steroidal hormones & Cardiac glycosides
Cholesterol Triterpenoid
A B
C DA B
C D
HO
E17
12
34
56
7
89
10
11 12
13
14 15
16
1718
19
2021 22
23
2425 26
27
4
20
OH
![Page 3: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/3.jpg)
Medicinal Value of Plant Steroids
• Important source of steroidal drugs
• Minor % prepared from animal steroids & total synthesis
• Norgestrel, C13 ethyl, totally synthetic (nothing in nature like this structure)
• Aromatic steroids & C19 nor steroids are animal e.g. Norethisterone
OH
5O 4 Norethisterone
HC CHH19
OH
5O 4 Norgestrel
H CH
CH
![Page 4: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/4.jpg)
Steroidal Saponins• Same triterpenoidal saponins characters• Less widely distributed in nature• Plant families Dioscoreaceae, Liliaceae, Agavaceae • Sapogenins 27 Carbons• Side chain of cholesterol modified to spiroketal or hemiketal• All have C25 stereoisomerisation• Sugars @ C-3 & some @ C-26• Less sugar molecules (Triterpenoidal saponins??)• > sugars result in > haemolytic effect• Raw material for the partial synthesis of steroidal hormones:
”Marker degradation”[Diosgenin →Progesteron; ONLY 5 step reaction!]
• Other raw materials: sitosterol, stigmasterol
![Page 5: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/5.jpg)
Dioscorea (Dioscoreaceae)
• D. alata & D. esculenta, D. rotundata edible• Roots of some spp are rich in steroids considered
bitter & inedible (Excellent source for drug manufacture)
• Diosgenin is major sapogenin (60% of steroidal production derived from diosgenin)
• Powdered Dioscorea (wild yam) root or extract as Rx for menopause (alternative to HRT) efficacy ????
• Diosgenin conversion in humans to progesterone
![Page 6: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/6.jpg)
Diosgenin• The aglycone of the saponin from the tubers of Dioscorea spp
(Dioscoreaceae) e.g. D. composita, D. mexicana• Important source for pharmaceutical industry as the starting
molecule of steroids synthesis e.g. progesterone, testosterone, cortisone
• Microorganisms introduce OH gp at specific position in steroid skeleton
O
O
O
22
25
26
3Diosgenin
O
OO
OH
OHOH
OHOH
O
O
D-Gluc
L-Rha
L-Rha
1 4
1 2
Alpha
Alpha
Dioscin
SpirostaneBeta 1
![Page 7: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/7.jpg)
Sterols
• Zoosterols: side chain 8C→Cholesterol• Mycosterols: side chain 9C →Ergosterol• Phytosterols: side chain 10C →Sitosterol
HO 5 6Cholesterol
ErgosterolSitosterol
![Page 8: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/8.jpg)
Cholesterol • Principle animal sterol • Alcoholic triterpene (lanosterol) converted to cholesterol
• Essential constituent of cell membranes in all animal tissues• Maintains membrane fluidity, microdomain structure & permeability• Precursor for steroid hormones, bile acids, Vit. D & lipoproteins “LDL & HDL”
(proteins + phospholipids carrying cholesterol in blood to tissues) • Associated with cardiovascular diseases, atherosclerosis,
hypercholesterolaemia, gallstone diseases (mostly cholesterol precipitated from bile)
HO 5 6Cholesterol
HOH
98 14
Lanosterol
H
3 CH3+2 H
![Page 9: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/9.jpg)
Medicinal Value of Cholesterol • Cholesterol biosynthesis inhibited by HMG-CoA reductase in
mevalonate pathway e.g. lovastatin and derivativesCholesterol is primary source for semi-synthetic medicinal
steroidsNatural sources for industry: • Brains and spinal cords of slaughtered cattle (by-product of
meat industry)• Extracted lanolin & fatty layer of sheep’s wool (lanolin
saponification provides alcohol fraction 34% cholesterol + 38% lanosterol/dihydrolanosterol
• Wool alcohols are used as ointment base
![Page 10: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/10.jpg)
Stigmasterol and sitosterolStigmasterol first isolated from calabar beans, then from soya bean oil
Both are raw materials for the semisynthetic production of steroids. Stigmasterol easily converted to progesteron which is in turn starting material for other steroids
HO
H
H
H
22 24
241242
STIGMASTEROLHO
H
H
H
22 24
SITOSTEROL
HH
RS
![Page 11: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/11.jpg)
Sitosterol• Analogue of stigmasterol with saturated side-chain • Sources: soya-bean and oils e.g. maize oil• Can not be degraded using chemical methods (no
double bond side chain)• Microbial degradation• Starting molecule for sex hormone synthesis
especially 19-norsteroids & spironolactones • Topical products for eczema, dry skin, burn scars• Hypocholesterolemic properties
![Page 12: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/12.jpg)
Vitamin D • Derived from Δ5,7-sterols • Chemically related to steroids (ring B open)• Cholecalciferol= Vit D3 from liver, milk, butter,
fish-liver; skin of man!• Ergocalciferol= Vit D2 by UV radiation of
ergosterol from yeast, overirradiation produces toxic substances: Toxisterols
• Vit D essential for the utilization of Ca and P• Overdose: renal calculi, metastatic calcification
![Page 13: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/13.jpg)
Vitamin D
HO
H
HO
UV
17
HO
HUV
17
HO
7-Dehydrocholesterol
Colecalciferol (Vit. D3)
Ergosterol
Ergocalciferol (Vit D 2)
![Page 14: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/14.jpg)
Vit D
• Vit D2 + Lumisterol → Vit D1
• Ergosterol (Pro Vit D2)→ Vit D2
• 7-dehydrocholesterol → Vit D3
• 22:23 dihydro-Vit D2 → Vit D4
• 7-dehydro-sitosterol → Vit D5
• 7-dehydrostigmasterol → Vit D6
• 7-dehydrocampesterol → Vit D7
![Page 15: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/15.jpg)
Cardiac Glycosides Cardiotonic glycosidesHistory of Traditional Use:Arrow poisonsHeart drugsRx of dropsy (accumulation of H2O in the body)Modern Medicine Applications:Rx of CHF (+ve inotropic effect) Supra-ventricular fibrillation
![Page 16: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/16.jpg)
Natural Sources
Botanical Origin:
Very limited distribution Mainly Asclepiadaceae & Apocynaceae Digitalis lanata, D. purpurea (fox gloves)
Scrophulariaceae الديجيتال Strophanthus kombe, S. gratus ApocynaceaeNerium oleander (Rose laurel) الدفلة ApocynaceaeHelleborus niger (Christmas Rose) Ranunclaceae
المجيد الميالد عيد زهرةUrginea maritima (Squill) Liliaceae العنصل Toad venoms
![Page 17: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/17.jpg)
Chemistry-Pharmacology Relation
Therapeutic action depends on :1. Structure of aglycone2. Type & number of sugar units attached3. Pharmacological activity is due to the aglycone but
modified by nature of sugar @C34. Sugar for transportation & pharmacokinetics5. Biosynthesized from cholesterol through
shortening and cleavage of the side chain followed by oxidation and cyclyzation
![Page 18: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/18.jpg)
Aglycone Classes• C23 & C24Characteristic of C. Glyc:1. A/B + C/D Cis2. B/C Trans3. 3β- OH, 14β- OH4. α,β- unsaturated lactone
ring @ C-175. Additional OH at C12&C166. C19:CH3/CH2OH/COOH 7. Glycosidic bond @ C-3 [Spirostane saponins co-occur
with cardenolide glycosides in D. purpurea]
O
HO
H
O
OH
H
H
Cardenolide (C 23)
12
3
4
5
6
78
910
1112
1314
15
16
1718
19
2120 22
23
HO
H OH
OH
H
O
O
2
3
4
56
8
9
1112
1314
15
16
1718
19
2120 22
2324
Bufadienolide (C24)
7
10
Cardenolide Lactone Ring
Bufodienolide Lactone Ring
![Page 19: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/19.jpg)
Sugar moieties in cardiac glycosides
Glucose andRare/uncomon sugars:• 6-deoxysugars• 2,6-deoxysugars• Their 3-O-methylethers• i.e: L-rhamnose, D-digitoxose, D-digitalose, D-
cymarose, D-diginose, D-sarmentose, …
![Page 20: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/20.jpg)
Glycones of cardiac glycosides
• 2,6-dideoxyhexoses (digitoxose & cymarose)• 6-deoxyhexoses (L-Rhamnose & D-Digitalose) • 3-Methylether hexoses (Digitalose & Cymarose) • D-Glucose, D-Fructose in 1ry Glycosides
O
OH
OH
23
45
6
OH
D-Digitoxose
O
OH
OH
23
45
6
OCH3
O
OH
OH
23
45
6
OCH3OH
D-DigitaloseD-Cymarose
![Page 21: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/21.jpg)
Digitalis purpurea glycosidesScrophulariaceae (Foxglove)
1ry Glycosides Series Aglycone
Purpureaglycoside-A 3β,-14β-diydroxylated
Digitoxigenin
Purpureaglycoside-B 3β,14β,16β-trihydroxylated
Gitoxigenin
Purpureaglycoside-E
(Glucogitaloxin)
16-formylgitoxigenin
Gitaloxigenin
![Page 22: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/22.jpg)
Purpureaglycoside-A & Digitoxin
OO
O
O
O
OO
O
O
O
HO
HO
HOHOOH
HO OH
OH
14
3
1612
H
H
Purpureaglycoside A
D-glucose
D-digitoxose
3
HD-digitoxoseD-digitoxose
Digitoxin
![Page 23: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/23.jpg)
Chemistry of D. lanata leaves (Scophulariaceae)
• Dried @ temp ≤ 60ºC to prevent glycosidic link hydrolysis
• > 60ºC result in dehydration @ C14 (inactive 14-anhydro comp.)
Cardiac glycosides: Fresh Plant: 1ry glycosides=Lanatosides A-E series (Aglycone + 3 digitixoses +Acetyl + Glucose)Dry Plant: 2ry Glycosides=Lanatosides loses terminal
glucose (Aglycone + 3 digitixoses + Acetyl)• Aglycone: Cardenolides [C23] in both
![Page 24: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/24.jpg)
Lanatosides1ry Glycosides
SeriesAglycone
Lanatoside-A3β,-14β-diydroxylated
Digitoxigenin
Lanatoside-B3β,14β,16β-trihydroxylated
Gitoxigenin
Lanatoside-C3β,14β,12β-trihydroxylated
Digoxigenin
Lanatoside-D3β,14β,12β,16β-tetrahydroxylated
Diginatigenin
Lanatoside-E16-formylgitoxigenin
Gitaloxigenin
![Page 25: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/25.jpg)
Differences between two Digitalis species
• The primary glycosides are not identical since in D. lanata -even the aglycon is the same- there is an acetyl group attached to the third sugar on OH at C-3
• Hydrolysis of the terminal glucose in D. lanata yields Acetyldigitoxin, acetylgitoxin, acetyldigoxin, acetylgitaloxin while in D. purpurea: Digitoxin, gitoxin, gitaloxin
• Digoxigenin and its glycosides found only in D. lanata (Lanatoside C)
• Digitoxin is the main secondary glycoside of D. purpurea while digoxin is the main secondary glycoside of D. lanata
![Page 26: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/26.jpg)
Digoxin
OO
O
O
OO
O
O
HO
HO
HO
HOOH
14
3
1612
H
H
3
H
HO
D-digitoxoseD-digitoxose
D-digitoxose
![Page 27: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/27.jpg)
Digoxin ProductionPharmaceutical industry: Deacetylated the 2ry glycoside from dry leaves of
D. lanata to produce digoxin OR Terminal glucose is removed first then
deacetylate 2ry glycoside from fresh plant Deacetylation first then hydrolysis of terminal
glucose is a 2nd option
![Page 28: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/28.jpg)
Digoxin
Lanatoside-C hydrolysed into acetyldigoxin by the action of β-glucosidase
Digoxin is the deacetylated derivative of 2ry glycoside from Lanatoside-C
Rx CHF & atrial fibrillation
OO
O
O
O
OO
O
O
O
HO
HO
OHOOH
O
HO OH
OH
14
3
1612
H
H
Lanatoside-C
D-glucose
3-acetyl-D-digitoxose
3
H
HO
D-digitoxoseD-digitoxose
Acetyldigoxin
![Page 29: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/29.jpg)
Medicinal Applications• Illegal to sell crude drug or extracts of Digitalis
(bacterial contamination of the leaves)• Digitalization is usually required • Drug monitoring through radioimmunoassay using
specific antibodies • Sheep derived digoxin-specific antibody fragments
for Rx of digoxin overdose• Digitoxin is highly lipid soluble, completely absorbed
from GI tract (95%); halflife time :7days• Digoxin less lipid soluble, GI absorption 75%; halflife
time:1.5 days• Polarity of cardiac glycosides↑, oral absorption↓
![Page 30: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/30.jpg)
Urginea maritima (Squill) • Bulb (formerly Scilla
maritima)• Family Liliaceae • Used as expectorant as
Gee’s linctus• Large doses toxicity
(vomiting & digitalis-like action on heart)
• Grows on Mediterranean & Canary Islands shores
![Page 31: Steroids Wide range of biologically active natural products e.g. sterols, steroidal saponins, cardioactive glycosides, bile acids, corticosteroids, mammalian](https://reader035.vdocument.in/reader035/viewer/2022062300/56649e2a5503460f94b17a4c/html5/thumbnails/31.jpg)
Proscillaridin A• Large number of cardiac glycosides of
bufadienolides class
Proscilaridin A
O
ORhamnose
O
OH
14
3
17