© intheskies / fotolia.com
KIT – The Research University in the Helmholtz Association
Dr. Clarissa Marquardt - Institute of Applied Computer Sciences (IAI)
www.kit.edu
Supporting Risk Assessment of Nanomaterials with quality-Approved Information DaNa Literature
Criteria Checklist
Institute of Applied Computer Sciences (IAI)2 08.11.2016
Nanotechnology……
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Applications?
Image source: fotolia.com
Institute of Applied Computer Sciences (IAI)3 08.11.2016
Nanotechnology……
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Effects on……
Environment
Worker
ConsumerSAFETY ?
Image source: fotolia.com
Institute of Applied Computer Sciences (IAI)4 08.11.2016
Nanotechnology……
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Effects on……
SAFETY ?
Environment
Worker
Reliable Source of
information?
Image source: fotolia.com
Institute of Applied Computer Sciences (IAI)5 08.11.2016
Literature on Nanotoxicology
Drastic increase in Number of Publications increases drastically
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
0
500
1000
1500
2000
2500
3000
3500
4000
1990 1992 1994 1996 1998 2000 2002 2004 2006 2008 2010 2012 2014
Nu
mb
er
of
Pu
blic
atio
ns
Year of Publication
Source: Nau K. et al (2016), The DaNa2.0 Knowledge Base on Nanomaterials – Communicating current nanosafety research based on evaluated literature data. Journal
of Materials Education Vol. 38 (3-4): 93-108 (2016)
Oberdörster 2005 “Nanotoxicology: An
Emerging Discipline Evolving from Studies
of Ultrafine Particles”
Institute of Applied Computer Sciences (IAI)6 08.11.2016
Quality & Usability of Publications
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Krug H.F. (2014). Nanosafety research-
are we on the right track? Angew Chem
Int Ed Engl, 53(46): 12304-12319.
Literature Review on 3 major uptake pathways &
comparison Instillation vs Inhalation
Majority of studies was poorly conducted
Characterisation of NM was insufficient
Applied dosage in most cases under overload
conditions
Results NOT applicable for risk
assessment!!!
Institute of Applied Computer Sciences (IAI)7 08.11.2016
Characterisation…..
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
„When things are large, they are what the are. When they are small,
it‘s a different game: they are what our measurements make them.”
(George M. Whitesides, No Small Matter. Science on the Nanoscale)
Institute of Applied Computer Sciences (IAI)8 08.11.2016
Characterisation…..
Key challenge for nanosafety assessment &
safe-by-design approaches is to
Understand which phys.-chem. properties are
driving the nanomaterials’ toxicity
Standardised testing methods & materials
Dose & dosimetry in Nanotoxicology
Naming & categorizing nanomaterials
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
„When things are large, they are what the are. When they are small,
it‘s a different game: they are what our measurements make them.”
(George M. Whitesides, No Small Matter. Science on the Nanoscale)
Fadeel B., Fornara A., Toprak M.S., Bhattacharya K.
(2015). Keeping it real: The importance of material
characterization in nanotoxicology. Biochem Biophys
Res Commun,. Doi: 10.1016/j.bbrc.2015.06.178
Institute of Applied Computer Sciences (IAI)9 08.11.2016
Requirements…
Quality standards for (nano)toxicological studies
Physico-chemical characterisation fit-for-purpose & relevant for
addressed purpose
Validated standard methods & reference materials
Relevant models & concentrations
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Institute of Applied Computer Sciences (IAI)10 08.11.2016
Short Overview
Communication of current nanotechnology safety research
Scientific Literature Review & Quality Management
Collecting – Evaluating & Processing of Nanosafety Information for Website
for Interested Laymen, Stakeholders, Scientists
Website www.nanoobjects.info
Support for German Nanosafety Research projects
NanoCare, NanoNature, ERA-Net SIINN
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Federal Office for the Environment FOEN
Federal Office of Public Health FOPH
Supported by
FKZ 03X0131
Supported by
Key Tasks of the DaNa - Knowledge Base Nanomaterials (DaNa = Database Nanomaterials)
Institute of Applied Computer Sciences (IAI)11 08.11.2016
External Experts
Project Team
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Core Team
Institute of Applied Computer Sciences (IAI)12 08.11.2016
Literature Quality Management
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
approved rejected
www.nanoobjects.info
Evaluation of peer-reviewed
literature with publicly available
quality criteria
Topics Human- & Eco-Toxicology
Sorting of approved & rejected
literature using the DaNa Literature
Criteria Checklist
> 800 quality-approved literature
citations on the website
Institute of Applied Computer Sciences (IAI)13 08.11.2016
DaNa Literature Criteria Checklist
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Mandatory & Optional Criteria
http
://w
ww
.na
no
pa
rtik
el.in
fo/f
iles/m
eth
od
ik/D
aN
a_
crite
ria
_ch
ecklis
t_2
01
5_
form
.pd
f
Institute of Applied Computer Sciences (IAI)14 08.11.2016
DaNa Literature Criteria Checklist
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Mandatory & Optional Criteria
Physico-Chemical
characterisation
http://www.nanopartikel.info/files/methodik/DaNa_criteri
a_checklist_2015_form.pdf
Institute of Applied Computer Sciences (IAI)15 08.11.2016
DaNa Literature Criteria Checklist
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Mandatory & Optional Criteria
Physico-Chemical
characterisation
Sample Preparation
http://www.nanopartikel.info/files/methodik/DaNa_criteri
a_checklist_2015_form.pdf
Institute of Applied Computer Sciences (IAI)16 08.11.2016
DaNa Literature Criteria Checklist
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Mandatory & Optional Criteria
Physico-Chemical
characterisation
Sample Preparation
Testing Parameters
Biological assays
dose & dosage
controls,…
General Aspects
Evaluation / Statistics
SOPs, Guidelines,…
http://www.nanopartikel.info/files/methodik/DaNa_criteri
a_checklist_2015_form.pdf
Institute of Applied Computer Sciences (IAI)17 08.11.2016
Standard Operating Procedures (SOPs)
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
DaNa SOP Template
Validated SOPs & Lab-Protocols from German NanoSafety Research Projects
Protocols from other projects (NANOMMUNE, V.I.G.O,…)
Institute of Applied Computer Sciences (IAI)18 08.11.2016
Project DaNa2.0 www.nanoobjects.info
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Institute of Applied Computer Sciences (IAI)19 08.11.2016
Conclusions
DaNa Literature Checklist – A tool for quality management of
publications
Increasing SOP collection
DaNa Knowledge base – Reliable & objective information source
Knowledge communication essential for any technology development
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
Institute of Applied Computer Sciences (IAI)20 08.11.2016
Thank you for your Attention !
Dr. Clarissa Marquardt NanoSafe 2016 - Grenoble
www.nanoobjects.info
@nano_info