DNA Repair
ActivatorsApoptosis
G1/S checkpointChromatin
modification
Apoptosis?DNA Repair
Apoptosis
ApoptosisCheckpoint
Intra S checkpoint
Intra S checkpoint
Intra S checkpoint
G1/S and G2M checkpoints
DNA Repair
G1 checkpoint
G2 checkpoint
Checkpoints
Artemis
Smc1
53Bp1
Rad9
FancD2
Mdc1
Rad17
Nbs1
H2AX
Chk2
Brca1Strap
p53
E2F1
Che1
Activated ATM Kinase
S31
S1423
S1387
S343
S278
S22
S957 S978
S272
S645S635
T68
S15
S203
S139S1981
S25?
S645
Kindly reviewed by Dr. Domenico Delia, Istituto Tumori, Milano, Italy
www.abcam.com
Targets for ATM phosphorylation
atm map 28/7/05 14:24 Page 1
ATM M ab78 WB, IPATM P ab91 WB, IF, IHC, IPATM M ab2618 WB, IFATM P ab17995 WB, IPATM P ab2631 WB, IPATM (phospho S1981) P ab2888 WB
Artemis P ab3834 WBArtemis P ab14289 WBArtemis P ab18319 WB
53BP1 P ab21083 WB
MDC1 P ab11169 WB, IFMDC1 P ab11170 WB, IPMDC1 P ab10948 WB, IF
H2A.X P ab10475 ELISA, WBH2A.X (phospho S139) P ab2893 WB, IFH2A.X (phospho S139) P ab11174 WB, IF
RAD9 M ab13600 WB, IHC, IP RAD9 M ab13885 WB, IHC, IP
RAD17 P ab2847 WBRAD17 P ab3620 WBRAD17 P ab9744 WB, IF
FANCD2 P ab2187 WB, IF, IPFANCD2 M ab5360 WB, IPFANCD2 (phospho S222) P ab18089 WB, ICC
p53 M ab26 WB, ELISA, FACS IHC-P,IHC-Fr, IP
p53 M ab28 WB, ELISA, FACS, RIA,IHC-P, IHC-Fr, IP
p53 P ab4060 IHC-P, IHC-Frp53 P ab16121 WB, IHC-P, IP p53 (phospho S15) P ab1431 WB, IHC-P, IP
Chk2 M ab3292 WB, IF, IPChk2 P ab5696 WBChk2 P ab8108 WBChk2 M ab18330 WB
SMC1 M ab16147 WB, IFSMC1 P ab9262 WB, ICC, IPSMC1 (phospho S957) P ab1275 WB, IP
Nbs1 P ab398 WB, IF, IPNbs1 M ab7860 WB, IPNbs1 (phospho S343) P ab15088 WB, IFNbs1 (phospho S343) P ab15089 WB
E2F1 P ab2960 IF, IP, EMSAE2F1 M ab4070 IHC-P, IHC-FrE2F1 M ab11950 WB
BRCA1 M ab16780 WB, ICC, IHC-P, IHC-Fr, IPBRCA1 M ab94 WB, IHC, IPBRCA1 P ab9141 WB, IPBRCA1 (phospho S1423) P ab2838 WB BRCA1 (phospho S1387) P ab2843 WB
KEY: M - Monoclonal P - Polyclonal
Western blot analysis of ATM (phospho S1981)ab2888
Antibody Clonality Datasheet Applications testedwww.abcam.com/
www.abcam.com
Antibody Clonality Datasheet Applications testedwww.abcam.com/
Lanes 1 - 2: ATM (phospho S1981) antibody at 1/100 dilution
Lanes 3 - 4: ATM (phospho S1981) antibody at 1/200 dilution
Lanes 5 - 6: ATM (phospho S1981) antibody at 1/500 dilution
Lanes 7 - 8: ATM (phospho S1981) antibody at 1/1000 dilution
Lanes 1, 3, 5, and 7: 15 ug whole cell extract from a humanlymphoblastoid cell line.
Lanes 2, 4, 6, and 8: 15 ug whole cell extract from a humanlymphoblastoid cell line treated with 6Gy of ionizing
radiation and harvested one hour later.
1:100
6Gy IR
ATM ser-1981
ATM
1:200 1:500 1:1000
This is just a selection of our
top ATM and ATM-related antibodies.
Our range contains over 2,500
Nuclear Signaling antibodies to
choose from! Find out more on our
Nuclear Signaling
www.abcam.com/nuclearsignal
ATM and ATM-related Antibodies
atm map 28/7/05 14:24 Page 2