Download - The King of Fruits: Durian
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The King of Fruits: Durian
Peter LeeVictor Ng
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Basics
• Location – Southeast Asian Islands• Thorny shell, Pungent Odor• Can kill on contact With your head from High altitudes
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Growth
• Pollination – Determined by Spider Hunters, various native bird species, and even bats.
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Growth
• Cultivation – Not an easy task due to dangers of fruit falling on the cultivator’s head upon his inspection of the trees unless he is careful and organized.
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DNA Extraction
• Fresh Durian Fruit Dissection Seed Removal Seed Dissection Remove the Embryo Grind the Embryo.
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DNA Isolation
• With the proper Buffers and equipment DNA isolation is based on general experience. There WILL be mistakes in the beginning.
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Dye
• The dye was combined with our isolated DNA simply by transferring it to a premade pebble of dye in another tube.
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PCRThe thermal cycler uses quick changes in temperature to break and merge strings of nucleotides. High temperatures break them apart, and cool temperatures match them with their corresponding strings.
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Thermal Cycler
• 24 Cycles• Duplicate/Clone DNA• 224 = 16,777,216
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Gel Electrophoresis
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Results????
• NONE yet due to complications with equipment and shortages.
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Conclusion
• Our primers have not arrived, and therefore we could not come to any conclusions about the Durian family tree and what makes them so diverse.