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Using The Bio-Plex Assay Builder
How to find the Assay Builder:
www.bio-rad.com Select Life Science Research Catalog Index Drop-Down:
Select Multiplex Suspension Array System
Select Bio-Plex Express and x-Plex Assays Select Assay Builder
Or
www.bio-rad.com/bio-plex/x-plex Select Assay Builder
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Catalog # Naming Scheme:
Z Products ordered through the Assay Builder Expressoptions (e.g. Z50005KMTI).
M Products ordered through the Assay Builder x-Plexoptions (e.g. M5000B6AVC).
X Products ordered for rat cytokines, phosphoprotein, andtotal targets (e.g. X70IQQDQHR).
171- Products ordered for phosphoprotein and total targetswhen only one target and one plate is ordered
(e.g. 171-V27145).
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Using The Bio-Plex Assay Builder (step-by-step)
Catalog Number(Youre not done yet. Must continue to Step 8)
5. obtain pricing
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3. Make target selectionsNotes:1. For human and mouse cytokines, selecting
only one target will automatically direct theuser to the Express option.
2.For phospho and total targets, selecting onlyone target will automatically direct the user to
the Off-The-Shelf Singleplex assay option.
2. Select package size1. Select group
4. view catalog #
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Using The Bio-Plex Assay Builder (step-by-step)
6. Fill in contactinformation
7. I plan to orderI plan to order will ensure the catalog # is available the next day. Generally speaking, the catalog #
will be available next day if the submit button is pressed before 2pm.
Just looking will eventually generate the catalog # with considerable delay.
8. Submit
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Two Bullet Screen (Step 7) Express Phosphoprotein Phosphoprotein Total
Rat Cytokine
Four Bullet Screen (Step 7) Human Cytokine Group I (two to ten targets selected) Human Cytokine Group II (two to ten targets selected)
Mouse Cytokine Group I (two to ten targets selected) Mouse Cytokine Group II (two to ten targets selected)
Or
6. Contact
7. I plan to orderCustom blend (x-Plex, pre-mixed) or Express (non-mixed)
8. Submit
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Using The Bio-Plex Assay Builder (step-by-step)
Final Screen(no action needed on this screen)
10. Next day - Call Customer Service to place order.Refer to the catalog # on the email when placing the order next day. If the Assay Builder wascompleted late in the day (generally speaking >2pm), may need to wait another day. Youre finished!
See Pages 5, 6, and 7 for example emails.
9. Auto-generated email shortly after pressing submit.The email will arrive shortly after pressing submit.
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Anatomy of the auto-generated email(example from an Express or x-Plex order)
Catalog #For current orderx if x-Plex order
PriceFor current order
Shipping Info
ExpressPrice list for 1-10 targets whenExpress ordering
Optional ProductsOrdering info for extra
filter plates and standards
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Anatomy of the auto-generated email(example from a Rat order)
Catalog #For current order
PriceFor current order
Shipping Info
RequiredOrdering info for additional
products required to run anassay
Optional ProductsOrdering info for Diluent Kit
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Anatomy of the auto-generated email(example from a phosphoprotein or total targets order)
Catalog #For current order
PriceFor current order
Shipping Info
RequiredOrdering info for additional
products required to run anassay
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Whats Needed To Run An Assay
xxStandards
xxAssay Buffer
xxDiluent (Sample)
xxDiluent (Standard)
xxSealing Tape
xxFilter Plate
xxStreptavidin-PE
xxDiluent (det Ab)
xxWash Buffer
xxxDetection Ab
xxxBead (magnetic)
Included inSingleplex Sets
Included inAssay BuilderExpress*
Included inAssay Builderx-Plex*
Needed to runan assay
Bio-Plex Pro Cytokines (human, mouse)
* All-in-one kit formatBlack Components of the panels listed below (as well as x-Plex and Express
Panels and Singleplex Sets Assays):Human: 8-. 17-. 21-, 27-, Th1/Th2Mouse: 8-, 9-, 23-, Th1/Th2
Blue Components of Cytokine Reagent Kit (diluents included)
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Whats Needed To Run An Assay
xStandards
Wash Buffer
Assay Buffer
Diluent (Sample)
Diluent (Standard)
Sealing Tape
Filter Plate
Streptavidin-PE
Diluent (det Ab)
xDetection Ab
xBead (non-magnetic)
Included inAssay Builderx-Plex*
Needed to run anassay
Bio-Plex Cytokines (rat)
* Rat cytokines is not offered in ExpressBlack Components of the 9-Plex A and x-Plex Panels and Singleplex
Sets AssaysBlue Components of Cytokine Reagent KitGreen Components of Diluent Kit
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Whats Needed To Run An Assay
Resuspension Buffer
Wash Buffer
Assay Buffer
xxControls
Factor 2
Factor 1
Cell Wash Buffer
Cell Lysis Buffer
Sealing Tape
Filter Plate
Streptavidin-PE
Diluent (det Ab)
xxDetection Ab
xxBead (non-magnetic)
Included inSingleplex Assays
Included inAssay Builderx-Plex*
Needed to run anassay
Bio-Plex Phosphoprotein
* Phosphoprotein is not offered in ExpressBlack Components of Phospho 3- and 5- Plex and x-Plex Panels andSingleplex Assays
Blue Components of Phosphoprotein Detection Reagent KitGreen Components of Cell Lysis Kit
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Whats Needed To Run An Assay
Resuspension Buffer
Wash Buffer
Assay Buffer
xxControls
Factor 2
Factor 1
Cell Wash Buffer
Cell Lysis Buffer
Sealing Tape
Filter Plate
Streptavidin-PE
Diluent (det Ab)
xxDetection Ab
xxBead (non-magnetic)
Included inSingleplex Assays
Included inAssay Builderx-Plex*
Needed to run anassay
Bio-Plex Total Targets
* Total Targets are not offered in ExpressBlack Components of Total Targets 5-Plex and x-Plex Panels and Singleplex
AssaysBlue Components of Phosphoprotein Detection Reagent KitGreen Components of Cell Lysis Kit
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Whats Needed To Run An Assay
xxControls
xDiluent (det Ab)
xSealing Tape
xFilter Plate
xStreptavidin-PE
xWash Buffer
xAssay Buffer
xxStandards
xDiluent (standard)
xDiluent (sample)
xxDetection Ab
xxBead (magnetic)
Included inAssay Kits*
Included inComplete Kits*
Needed to runan assay
Bio-Plex Pro Human Acute Phase
* Disease research assays are not ordered through the Assay BuilderBlack Components of 4- and 5-Plex PanelsBlue Components of Acute Phase Pro Reagent KitGreen Components of Acute Phase Diluent Kit
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Whats Needed To Run An Assay
xxControls
xWash Buffer
xAssay Buffer
xxStandards
xDiluent (standard)
xDiluent (sample)
xSealing Tape
xFilter Plate
xStreptavidin-PE
xDiluent (det Ab)
xxDetection Ab
xxBead (magnetic)
Included inAngiogenesis Panel*
Included inComplete Kits*
Needed to runan assay
Bio-Plex Pro Human Angiogenesis
* Disease research assays are not ordered through the Assay BuilderBlack Components of 9-Plex PanelBlue Components of Pro Angiogenesis Reagent KitGreen Components of Human Serum Diluent Kit
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Whats Needed To Run An Assay
xxxxControls
x
x
x
Included in
(2-Plex Panel)
x
x
x
Included in(12-Plex Panel)
x
x
x
Included inSingleplex Assays
(Adiponectin, Adipsin)
xWash Buffer
xAssay Buffer
xStandards
xDiluent (serum)
xDiluent (serum-free)
xSealing Tape
xFilter Plate
xStreptavidin-PE
xDiluent (det Ab)
xDetection Ab
xBead (magnetic)
Included inComplete Kits
(12- + 2-Plex)
Needed to run anassay
Bio-Plex Pro Human Diabetes
* Disease research assays are not ordered through the Assay BuilderBlack Components of 12- and 2-Plex Panels and Singleplex AssaysBlue Components of Pro Diabetes Reagent KitGreen Components of Pro Diabetes Diluent Kit
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Whats Needed To Run An Assay
xxControls
xDiluent (det Ab)
xWash Buffer
xAssay Buffer
xxStandards
xDiluent (serum)
xSealing Tape
xFilter Plate
xStreptavidin-PE
xxDetection Ab
xxBead (magnetic)
Included in
7-Plex Panel*
Included inComplete Kits*
Needed to run anassay
Bio-Plex Pro Human Isotyping
* Disease research assays are not ordered through the Assay BuilderBlack Components of 7-Plex PanelBlue Components of Pro Isotyping Reagent KitGreen Components of Pro Isotyping Diluent Kit
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Whats Needed To Run An Assay
xWash Buffer
xAssay BufferxControls
xStandards
xDiluent (plasmastandard)
xDiluent (serumstandard)
xDiluent (sample)
xSealing Tape
xFilter Plate
xStreptavidin-PE
xDiluent (det Ab)
xDetection Ab
xBead (magnetic)
Included inAll-In-One*
Needed to run anassay
Bio-Plex Precision Pro Human Cytokine 10-Plex
* Precision Pro is not ordered through the Assay BuilderBlack All components included in all-in-one kit format
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