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VIB Core Facilities in a Nutshell Geert Van Minnebruggen, Ph.D – Integration Manager
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VIB ...an institute without walls
GENT
ANTWERPEN
LEUVEN BRUSSEL
▪ ± 75 research groups
▪ ± 1300 co-workers
▪ ± 400 PhD-students
▪ ± 250 postdocs
‘VIB – structure’
▪ 8 departments +
▪ 7 (8) institutional core facilities
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VIB ...a centre of excellence
‘VIB - Mission/KPI’s’
• Frontline biomolecular Research (+training)
- 60 papers top journals
- 180 papers HR journals
- 50 PhD’s
• Technology Transfer
- 25 patent applications
- 7M€ industrial income
- start-up
• Public Outreach
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GENT
ANTWERPEN
LEUVEN BRUSSEL
Core Facilities ...a key asset
‘VIB - Institutional Core Facilities’
▪ Users: VIB – academic - profit
▪ Fee for service (marginal/competitive pricing)
▪ All-in service: From design till data
▪ Subject to expert reviews every 3 years
▪ Centralized/decentralized mgnt model (Head of Core Facilities)
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‘Centralized – Decentralized management model’
Geert Van Minnebruggen Head Core Facilities
Peter Verhasselt Head Nucleomics core
Herwig Van Marck Head BioInformatics Training and Service Facility
Jurgen Haustraete Head Protein Service Facility
Dominique Audenaert Head Compound Screening Facility
Reza Gassabeh Head Nanobody Service Facility
Kris Gevaert Head Proteomics Expertise Center
Chris Guérin Head Ghent platform Bio Imaging Core
Sebastian Munck Head Leuven platform Bio Imaging Core
Alex Botzki Lab Informatics Expert
Core Facilities ...a key asset
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• Located in Leuven - UZ Gasthuisberg, O&N4
• Managed by Peter Verhasselt
• Team: 8 FTE’s
• Services:
– Affymetrix & Agilent chips
– NanoString nCounter
– 454 GS-FLX/Junior
– Illumina Myseq
– Ion-Torrent PGM
– Library prep (SPRI works; Apollo 324, Nextera (XT))
– Lease mode access to emerging platforms
– Access to Illumina HiSeq2000; PacBio
• Advice in experimental set-up and data-analysis
• Bioinformatics support
Nucleomics core
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• Located in Ghent - VIB HQ
• Managed by Herwig Van Marck
• Team: 4 FTE’s
• Services:
– Bioinformatics training
(IPA; CLC Bio, CG, Ariadne; EnsEMBL..)
– Software management/benchmarking
(CLC Bio; RT-G; Spotfire;..)
– Data mining
– Projects, e.g. 100 Genomes; Flanders Trio’s
– Galaxy
BioInformatics Training and Service Facility
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• Located in Ghent – department Molecular Biomolecular Research
• Managed by Jurgen Haustraete
• Team: 4 FTE’s
• Services:
– Production & purification recombinant proteins
Fastscreen: expression vector optimalization
Fastfold: recovery of proteins from inclusion bodies
Fastclear: optimalization of purification
Protein Service Facility
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• Located in Brussels – department Molecular and Celluar Interactions
• Managed by Reza Ghassabeh
• Team: 3 FTE’s
• Services offered:
– Nanobodies (Nbs) for a given antigen
– Engineered Nbs (tags, bivalent nanobodies, ...)
Nanobody Service Facility
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• Located in Ghent – department Plant Systems Biology
• Managed by Dominique Audenaert
• Team: 3 FTE’s
• Provides (VIB) researchers access to high-throughput small molecules and (siRNA) screenings and libraries
• Services:
– Assay development/validation + high-throughput small molecule/RNAi screens
– Post-screen validations
Compound Screening Facility
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• Located in Ghent, Rommelaere Institute
• Managed by Kris Gevaert
• Team: 2 FTE’s
• Services:
– Targeted/high-throughput proteomics (3 Orbitrap, Q-ToF, Maldi-MS/MS, ion-trap;..)
COFRADIC
Phosphoproteomics
SRM
Label-free analyses
...
Proteomics Expertise Center
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• Two platform sites - Ghent respectively Leuven
• Managed by Chris Guérin (Ghent) respectively Sebastian Munck (Leuven)
• Team: 8 FTE’s
• Services:
– Conventional Light & super resolution Microscopy
– SEM/TEM (including tomography)
– Serial Block Face SEM (i.e. Gatan 3View and FIB)
– Development 3D Correlative Light Electron Microscopy (3D-CLEM)
Bio Imaging Core
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• VIB HQ
• Alex Botzki
• Expertises:
– ELN
– OMERO
– LIMS
Lab Informatics Expert