Protein Arrays Detect protein-protein interactions Identify substrates of protein kinases Most commonly used for antibody
detection Used in biomedical applications to
determine presence/ amount of proteins in biological samples
Protein Arrays More diverse than DNA/RNA Cellular physiology Disease and cancer at the proteomic level
E-beam Lithography
Scanning a beam of electrons in an organized fashion across a surface covered with a film
Exposed/non-exposed regions can be selectively removed
E-beam comes from the Scanning Electron Microscope (SEM)
Concept
5
+
PMMA
Silicon Wafer+ + + + + + + + + + + + + + + + + + + + ++ + + + + + + + + + + + ++
+
Beads-COOH
IgG BSA
Silicon Wafer ++++++++++++++++++
PMMA
Silicon Wafer ++++++++++++++++++++
180nm
90nm50nm
Limitations
Proteins and antibodies are sensitive to environment
Low density nanoarray Multiple molecules at defined positions
when developing a multi-component assay
Biomarkers present at low concentrations
www.the-scientist.com/article/display/11822
Conclusion
Fabricated high-density, multi-component nanoarray through E-beam lithography and size-dependent self-assembly
Detected mouse IgG in array format through fluorescence properties
Future Directions
Detect Octamer-4 and other proteins under a multi-component nanoarray from real culture media
Reduce array scale to detect single molecule
Lehnert et al. J. Cell Science (2004)