dr. jeremy van boening - field perspective – sample handling & collection
TRANSCRIPT
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Trichomoniasis
Field Perspectives
Jeremy VanBoening, DVM
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Trichomoniasis Involvement
• Clinical Practice
• Nebraska Cattlemen-NCBA
• Nebraska Veterinary Medical Association
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Sample Collection
Texas A&M Trich Sample Collection Video
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Sample Collection ChallengesSurvey of 10 D-Labs 10-14-2013
Lab IDDo the samples need to be
incubated?
Do the samples need to be put on ice
when shipped?How many samples can be pooled? Preferred collection medium?
A Yes 24hrs Yes Up to 5 tubes
B No No, dark and warm packaging Up to 5 InPouch
C No, room temp No Up to 5, but not recommended InPouch
D
You can if you want, just state on
submission form if you incubated
samples or not. Have to receive
samples within 72hrs of collection.
If cold outside put a thawed ice pack. If
hot outside put ice pack but not directly
on samples
Up to 5 InPouch or tubes
E No, room temp, ship same day No No pooling InPouch
FYes or No, state on form if you did.
Has to arrive at lab within 120hrs
after collection.
No Up to 5 InPouch
G No cold pack Up to 5 Scraping in 5ml of Saline placed in tube.
H No No Up to 5 InPouch
I No No, room temp No pooling InPouch
J No, ship overnight Yes Up to 3 wash in red top tube with saline
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Summary of PCR Related Questions
Incubation Yes= 2 No=6 Your Choice=2
Ship on Ice Yes=2 Room Temp=6 Other=2
Pooling Up to 5=7 No Pooling=2 Up to 3=1
Preferred Collection Media In Pouch=6 Saline=2 Tubes=2
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Sample Collection Challenges
Restraint and Safety
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Is this safe??
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What Collection Method
1. preputial scraping with pipette
2. preputial scraping using thrichit
3. saline rinse
4. swap or scraping of the penis
NO BLOOD for Either PCR or Culture
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Sample Collection Challenges
Cleanliness
Field Collection Area
Scissors (Disinfect or Not and with what?)
Access to saline for rinsing
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Dirty Samples
• Rarely if Ever Return Positive
• Recent Study Hustun, etal. MSU
– 20 Samples w/ bacteria injected at increasing #’s
– 20 Samples clean (Only T. Foetus Added)
– 0 of 20 with bacteria ever recorded as positive
– 20 of 20 w/out bacteria had at least one positive recording
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Sample Collection ChallengesProper Collection Media
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Sample Preparation
Depends on the D-Lab!!
Collection Medium (In-Pouch, Tubes, Saline)
Incubate or Not
• Depends on D-Lab, PCR or Culture,
How should the sample be packaged?
• On Ice, Room Temperature, Or Depends on the Weather
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Transportation Issues
• Rural Access to Timely Reliable Transportation
• How to package and send samples?
– How long can they be in transit and at what temperature?
– Sensitivity and Specificity go down for both culture and PCR Hustun etal MSU
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How Do We Get Better????
Standardization
1. Adopt the Best Diagnostic Testing Available (qPCR) Using Chemical-Lysis and IAC.
2. Promote SOP’s for Sampling, Sample Prep, and Shipping
3. Increased Veterinarian/Producer Awareness
4. Standardized Regulations