May 15, 2017

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Duke Global Health Institute One Health: An Introduction to Entomology, Zoonotic Diseases,

and Food Safety GLHLTH 735

Summer 2017, 3 Credit Hours

Instructor Information Course director: Gregory C. Gray, MD, MPH Infectious Diseases Division Hanes House, Rm 254 Durham, North Carolina 27710 (919) 684-1032 gregory.gray@duke.edu Office hours arranged Other instructors: Various Course Description This course will introduce public health students to entomology, zoonotic diseases, and principals of modern food safety. Students will learn methods for conducting studies of mosquitoes and ticks, controlling zoonotic diseases, and protecting the food supply. A special focus will be upon modern food safety techniques in meat, dairy and produce production. Lectures will be complemented with considerable laboratory or field work. Course Pre-requisites One of the following courses (or equivalent): General Microbiology Principles of Infectious Diseases Epidemiology of Infectious Diseases

Date Time Room / field activity site 5/27/2017 Sat 10:00am-2:00pm Field Auditorium (room 112) Environment Hall

5/29/2017 Mon 8:00am-5:00pm Wet laboratory room Environment Hall 2012 /Duke Forest

5/30/2017 Tue 8:00am-5:00pm Duke Forest/ wet laboratory room Environment Hall 2012

5/31/2017 Wed 8:00am-2:30pm Field Auditorium (room 112) /403 W.Balfour Ave Asheboro NC /Beef processing plant visit

2:30pm-5:00pm Field Auditorium (room 112) Environment Hall 5:30pm-7:00pm Field Auditorium (room 112) Environment Hall 6/1/2017 Thu 9:00am-12:00pm Field Auditorium (room 112) Environment Hall 1:30pm-5:30pm Field Auditorium (room 112) Environment Hall 6/2/2017 Fri 9:00am-1:00pm Modern dairy production visit 1:00pm-5:00pm Field Auditorium (room 112) Environment Hall

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Course Objectives and/or Goals After completing this course, the student should be able to:

• Have a fundamental understanding of methods for mosquito collection, identification, and control;

• Be familiar with methods for tick collection, identification, and control; • Have an appreciation for zoonotic disease prevention and control in the

agricultural industry; • Be familiar with modern methods for monitoring food-borne pathogens.

Course Materials

Required textbooks: • David L. Heymann, MD, ed., Control of Communicable Diseases Manual

(CCDM), 19th edition, 2008, ISBN 087553189X • Service, M.W. Medical Entomology for Students, 5th Edition, 2012, ISBN:

978-1107668188 Course Requirements/Evaluation/Grading Students will be evaluated by their class participation (33%) and a 90-minute,

open book, open notes, examination (33%), and a 7-10 page term paper on a single zoonotic disease problems the student’s home country (33%) due by mid July. To earn a B for class participation, students should fully participate and attend every session. To earn an A in classroom participation, students must attend each session and demonstrate that they prepared for lectures/fieldtrips beforehand (through reading assigned text and interacting with the lecturers).

Percentage or points earned in class

93%-100%

90%-92%

87%-89%

83%-86%

80%-82%

77%-79%

73%-76%

70%-72%

Below 70%

Letter Grade equivalent

A A- B+ B B- C+ C C- F

Letter Grade A A- B+ B B- C+ C C- F NC

Grade Points 4.0 3.7 3.3 3.0 2.7 2.3 2.0 1.7 0.0 0.0

For greater detail on the meaning of letter grades and university policies related to them, see https://registrar.duke.edu/student-records/how-calculate-gpa

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Topical Outline Day/Lecture Title Lecturer Readings (pages)

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Saturday Field Auditorium Environ Hall 10-10:45am

Welcome, disease threats and population densities Gray

2 11-11:45am Zoonotic influenza and occupational risk part I Gray Bauerfeind 127-133

3 12-12:45pm Zoonotic influenza and occupational risk part II Gray

12:45-1:15pm Lunch

4 1:15-2pm Zoonotic influenza and occupational risk part III Gray

Monday Wet laboratory room Environ Hall 2102 8-8:10am

Introduction to vector biology training

Michael Reiskind

5 8:10–8:50am Vector biology: understanding and interruption

Michael Reiskind None

6 9:00-9:45am Mosquito biology

Michael Reiskind Service 1-82

7 9:45-10:30am Major mosquito borne diseases Michael Reiskind

8a 10:30-11:00am Filth flies and their role in human health

Michael Reiskind Service 139-173

8b 11:00-11:45pm Mosquito morphology and dissection

Michael Reiskind

12:00-12:30pm Lunch break Michael Reiskind

9 12:30-1:20pm Tick biology and life cycles Michael Reiskind Service 226-250

10 1:30-2:00pm Tick-borne diseases Michael Reiskind

11 2:00-2:45pm IVM: Control of mosquitoes and ticks – overview of approaches

Michael Reiskind

3:00pm Ride bus from lab room to Duke Forest

Duke Forest 3:10pm-4:30pm

Field session: Mosquito surveillance methods; Adult mosquito trapping techniques, mosquito trap set up—Duke Forest

Michael Reiskind

5:00pm Ride bus to hotel

Tuesday 7:15am

Ride bus from hotel to Duke Forest

Tuesday Duke Forest 8:00am-8:30am

Field session: Collection of adult mosquito traps—Duke Forest

Michael Reiskind

8:30am-9:30am

Field session: Dipping and collection techniques for mosquito immatures —Duke Forest

Michael Reiskind

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Day/Lecture Title Lecturer Readings (pages)

9:30am-12:00pm

Field session: Tick sampling methods: tick drag, CO2 bait—Duke Forest

Michael Reiskind

12:00 pm-1:00pm

Ride bus to wet lab – lunch break

Michael Reiskind

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Lab session Wet laboratory room Environ Hall 2102 1:00pm-2:15pm

Preliminary identification of wild caught mosquitoes (larvae, adults)

Michael Reiskind

13 2:30pm-4:00pm Lab session: Preliminary ID of ticks

Michael Reiskind

14 4:00-4:30 Summary of vector-borne diseases and their control

Michael Reiskind

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Wednesday Field auditorium 8am

The environment and zoonotic diseases Gray

16 9am

Bus departure at 9AM from Environmental Hall An introduction to meat science with intro to HACCP (lecture on the bus with video)

Dana Hanson

10:30-12:00

Beef production site visit Randolph Co. Packing in Asheboro, NC 403 W Balfour Ave, Asheboro, NC 27203, Tel (336) 672-1470. Bring lunch and water. Wear closed toe shoes and long pants. No jewelry of any kind can be worn in plant. Leave phones on Bus.

Dana Hanson

12:00-12:30 Lunch on the bus ride back to Duke

17 12:30-2:30 Review tour and discuss beef zoonoses (lecture on bus)

Dana Hanson

Read McDaniel CJ, et al. Humans and Cattle: A Review of Bovine Zoonoses, Vector-Borne and Zoonotic Diseases, 2014;14:1-19

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Field Auditorium Environ Hall 2:30-3:30pm

Commercial fishing industry and aquaculture

Jay Levine

Waltzek TB et al. Marine Mammal Zoonoses: A Review of Disease Manifestations. Zoonoses and Public Health, 2012:59:521-35??

19 3:30-4:30pm Seafood associated pathogens Jay

Levine

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Thursday Field Auditorium Environ Hall 8-9am

US poultry industry overview with emphasis on disease prevention I

Michael Martin 195-8, 206-11,219-23, 230-31

21 9-10 US poultry industry overview with emphasis on disease prevention II

Michael Martin

22 10-11 Select zoonotic diseases of poultry and OIE reportable diseases

Michael Martin

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Day/Lecture Title Lecturer Readings (pages)

23 11-12 Zoonotic diseases and wildlife Suzanne Kennedy Stoskopf

315-17

Lunch on the bus ride back to Duke

24 1-2pm Zoonotic infections associated with domestic pets (hookworm, toxoplasmosis, salmonellosis)

Gray Bauerfeind 110-17, 159-60, 199-201, 242-44, 25-61, 293-6, 339-41, 365-62, 395-9, 428-9, 432-34

25 2-3pm Swine zoonoses Gray

Khan SU, Atanasova KR, Krueger WS, Ramierez A, Gray GC. Epidemiology, Geographical Distribution, and Consequences of Swine Zoonoses: A Narrative Review Emerging Microbes and Infection, 2014; May;52(5):1762-5. doi: 10.1128/JCM.03522-13. Epub 2014 Mar 5.

26 3-4pm Other recently emerged zoonoses; explain term paper Gray Bauerfeind 141-7

Friday 8am

Bus departs Environment Hall bus stop at 8AM Gray

27-28 8:30-11:30am

Milk production and food safety lecture in classroom. Tour of NCSU Dairy Education Unit at 3437 Lake Wheeler Rd, Raleigh. Outbrief after tour in classroom. Bring lunch and water. Wear closed toe shoes. Leave jewelry on bus.

Harrison Dudley Bauerfeind 191-5, 276-9

11:30 Depart farm and return to Environment Hall Gray

12-1pm Lunch

29-30

Field Auditorium Environ Hall 1-3pm

Small group zoonotic disease exercise Gray

Friday 3-5pm Final exam (90 minutes, multiple choice, open notes) bring calculator

Gray

Duke Honor Code Duke University is a community dedicated to scholarship, leadership, and service and to the principles of honesty, fairness, respect, and accountability. Citizens of this community commit to reflect upon and uphold these principles in all academic and non-academic endeavors, and to protect and promote a culture of integrity. To uphold the Duke Community Standard:

• I will not lie, cheat, or steal in my academic endeavors; • I will conduct myself honorably in all my endeavors; and • I will not act if the Standard is compromised.

Students are encouraged to review the Duke Honor Code: (http://www.integrity.duke.edu/new.html)

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Plagiarism Plagiarism, of any kind, is not be acceptable and will result in an automatic failure and possible additional disciplinary action. See Duke Guidelines for plagiarism: http://library.duke.edu/research/plagiarism Attendance Policy Attendance is mandatory. Policy Related to Make-up Exams or Other Work Attendance and Make-up Work – I expect you to attend and be prepared to participate in all class sessions. Personal issues with respect to class attendance or fulfillment of course requirements will be handled on an individual basis.