e-cadherin and beta-catenin signaling and metastasis - … · e-cadherin and beta-catenin signaling...
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Figure 14.14c The Biology of Cancer (© Garland Science 2007)
E-cadherin and Beta-catenin signaling and metastasis
Rip-Tag transgenic
Mouse pancreatic
Cancer model
Green: cadherin
Red: catenin
Yellow-green area:
tumor in situ
Red area:
invasion front
Figure 14.14a The Biology of Cancer (© Garland Science 2007)
E-Cadherin stains strongly
in the core of a colon tumor. E-Cadherin stains lightly
at the edge of invasion front.
Figure 14.14b The Biology of Cancer (© Garland Science 2007)
Beta-catenin at membrane and cytoplasm
at the core of the tumor Beta-catenin in nuclei at the invasion front
Figure 14.15a The Biology of Cancer (© Garland Science 2007)
Twist induces EMT of MDCK cells and expression of EMT protein markers
Figure 14.16a The Biology of Cancer (© Garland Science 2007)
Normal melanocyte
Express E-cad
Interact with
keratinocyte
Figure 14.16b The Biology of Cancer (© Garland Science 2007)
Melanoma cells:
Expreass N-cad
Interact with
Fibroblasts and
Endothelial cells
Figure 14.19c The Biology of Cancer (© Garland Science 2007)
Transformed human
mammary epithelial cells
Implanted into SCID mouse
red
green
Figure 14.19b The Biology of Cancer (© Garland Science 2007)
Invading tumor cells express EMT marker laminin-2r
Figure 14.17a The Biology of Cancer (© Garland Science 2007)
Colorectal carcinoma cells
Red: cytokeratin 18 green: basement membrane
GFP-labeld MMTV-PyMT tumor cells injected via tail vein
mesenchymal epithelial
metastatic cancer cells in lung
Figure 14.18 The Biology of Cancer (© Garland Science 2007)
Similarity in biomarker expression between primary and metastasized tumors
Figure 14.20e The Biology of Cancer (© Garland Science 2007)
Detroit 562
carcinoma cells
Green:
TGF-beta
by stroma
Red:
Integrin by
cancer cells
Cancer-stroma
Feedback loop
Figure 14.21 The Biology of Cancer (© Garland Science 2007)
NF-kb signaling is needed for TGF-beta induced EMT markers
Figure 14.22a The Biology of Cancer (© Garland Science 2007)
Op/op: cannot make CSF-1 needed to recruit macrophages
Figure 14.22b The Biology of Cancer (© Garland Science 2007)
CSF -/- CSF +/-
CSF is essential for macrophages, which promote invasion.
MMTV-
Py-MT
Mouse
tumor
CSF:
Cancer
asso-
fibroblasts
Figure 14.22c The Biology of Cancer (© Garland Science 2007)
Ability of Py-MT tumor cells metastasize to lung with or without CSF-1
Figure 14.23a The Biology of Cancer (© Garland Science 2007)
Expression of complementary
molecules by cancer cells and
tumor asso-macrophages
Figure 14.23b The Biology of Cancer (© Garland Science 2007)
Macrophages promote breast cancer cells to move upward into collagen.