e. coslim - 2012.igem.org
TRANSCRIPT
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
32
Genes from E. coli and S. enterica were used
Placed downstream PfadR
Device I: Fatty Acid Degradation
34
In Vivo Experiment
Overexpression of FadE and FadD had no significant changes
Overexpression of FadL showed about 1.3 degradation of fatty acid
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
39
Device II: Cellulose Synthesis
AdrA was taken into
consideration
Genes from E.coli
Placed downstream Pcar
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
Device III: Colonization
A magic molecule: c-di-GMP
Modified from Hengge, Nature review Microbiology, 2009
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
Human Gut Microbiota
E.coslim
Human Gut
Microbiota
Huge quantity, diversity, complexity
Related to life activities Digestion, immunity, mental health
Forgotten organ
Gut microbiota is related to obesity
·Two major phyla of microbiota:
Firmicutes and Bacteriodetes
·Their proportion is related to
obesity.
·Firmicutes to Bacteriodetes:
High Obesity
Low Normal
Regulate the microbiota to make you slim!
Modeling on microbiota regulation
Simulation result in
obese people before
the colonization of
E.coslim
After colonization: different situations
Microbiota die out Fail to colonize
No significant change Colonization and regulation
Microbe proportion
Proportion of Firmicutes
decreases while that of
Bacteriodetes rises up
significantly after colonization
of E.coslim.
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
Outline
• Introduction
• Sensing
• Device I: Fatty Acids Degradation
• Device II: Cellulose synthesis
• Device III: Colonization
• Human Gut Microbiota Modeling
• Safety
• Human Practice
Survey
Most of the students hold negative opinions toward
Genetically Modified Organisms (GMOs).
harmful safe
Achievements
• Two synthetic promoters PfadR and Pcar
• In vivo and in vitro experiments on fatty
acid degradation
• Cloning and testing the function of
cellulose synthetase genes
• Modeling on gut microbiota
• An open software Fancy
• 66 new biobrick parts
Acknowledgement
• We thank Professor Jianbo Wang, and Zhixiong Xie for leading us
• We thank Dr. Xiangdong Gao, Mengxiang Sun, Ling Zheng, Xiaofeng Tang, Tiangang Liu, Yulan Wang, Lijia Li, Hongbin Shu, Xiaodong Zhang, Wentao Ma, Lin Guo, Daisuke Kiga and John Cronan for advices and kind instruction
• We thank Dundee iGEM team and Tokyo tech iGEM team for providing and preparing us cDNA and plasmids
Acknowledgement
• We specially thank Professor Yan Zhou, Yan Wu and Xiangdong Chen for their kindness to provide research equipments and reagents.
• We thank Yan Long, Jing Che, and Xiaoyan Li for ordering reagents and equipment.
• We thank former WHU iGEMer Chao Zhang, Jing You, Zurong Wan, Fei Shu, Meng Zhang, Yun Yan for their advices and supports.