early version of a project storyboard...sham group left eye-sham injection right eye-nothing c419 le...
TRANSCRIPT
![Page 1: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/1.jpg)
Early Version of a Project Storyboard
![Page 2: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/2.jpg)
Sham Group Left Eye-Sham Injection Right Eye-Nothing
C419 LE slide 5
C419 LE slide 9
C420 RE slide 2
C419 RE slide 8
![Page 3: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/3.jpg)
PBS Group Left Eye-PBS Injection
C422 LE slide 2
C423 LE slide 4
Right Eye-Nothing
C422 RE slide 3
C422 RE slide 4
C423 RE slide 2
![Page 4: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/4.jpg)
Microbead Group Left Eye-Microbead Injection
C426 LE slide 5
C427 LE slide 5
C426 RE slide 6
C426 RE slide 8
Right Eye-Nothing
C427 LE slide 6
C427 RE slide 2
C428 RE slide 5
![Page 5: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/5.jpg)
IBA 1 percent coverage
![Page 6: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/6.jpg)
GFAP Percent Coverage
![Page 7: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/7.jpg)
Same Data in a Later Version of the Project Storyboard
![Page 8: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/8.jpg)
IBA Percent Coverage
Figure 4: The microglia marker Iba1 (red) in confocal micrographs of optic nerve sections (A-F). Microglia in sham injected (A), sham contralateral (B), PBS injected (C), and PBS contralateral (D) are sparse and not very reactive. Microglia in both microbead injected (E) and microbead contralateral (F) eyes have enlarged somas and retracted processes, both signs of microglia activation. Scale bar represents 60um. Iba1 percent coverage data for optic nerve sections (G). Microbead injected eyes have significantly increased coverage compared to sham and PBS injected eyes. Microbead contralateral eyes have significantly increased coverage compared to PBS contralateral eyes. An asterisk represents a P value < 0.05.
![Page 9: Early Version of a Project Storyboard...Sham Group Left Eye-Sham Injection Right Eye-Nothing C419 LE slide 5 C419 LE slide 9 C420 RE slide 2 C419 RE slide 8. PBS Group Left Eye-PBS](https://reader033.vdocument.in/reader033/viewer/2022051604/5ffaf951455996789840e5cb/html5/thumbnails/9.jpg)
GFAP Percent Coverage
Figure 6: The astrocyte marker GFAP (green) in confocal micrographs of optic nerve sections (A-F). Scale bar represents 60µm. GFAP percent coverage data for optic nerve sections (G). Microbead contralateral optic nerve sections (F) have significantly less GFAP coverage than both sham contralateral (B) and PBS contralateral (D) optic nerve sections. There were no significant differences between injected eyes. An asterisk represents a P value < 0.05.