ectomycorrhiza inside root intercellular hyphae does not enter cells outside root thick layer of...
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Ectomycorrhiza
Inside root• Intercellular hyphae
• Does not enter cells
Outside root• Thick layer of hyphae around root
• Fungal sheath• Lateral roots become stunted
• Hyphae•Mass about equal to root mass
Forms extensive network of hyphaeeven connecting different plants
Ectomycorrhizal root tip
Mantle Hyphae
Hartig Net
Why mycorrhiza?
• Roots and root hairs cannot enter the smallest pores
Why mycorrhiza?
Root hair Smallest hyphae
• Roots and root hairs cannot enter the smallest pores
• Hyphae is 1/10th diameter of root hair
• Increased surface area
•Surface area/volume of a cylinder: SA/vol ≈ 2/radius
Inoculated with mycorrhizae
Not inoculated with
mycorrhizae
Why mycorrhiza?
• Roots and root hairs cannot enter the smallest pores
• Hyphae is 1/10th of root hair
• Increased surface area
• Extension beyond depletion zone
Why mycorrhiza?
• Roots and root hairs cannot enter the smallest pores
• Hyphae is 1/10th of root hair
• Increased surface area
• Extension beyond depletion zone
• Breakdown of organic matter and transfer of its N to host plant.
C – C – NH2 --> C – C + NH3
Are mycorrhizae always beneficial?
Probably not!!
Mutualistic Neutral Parasitic
Mycorrhizal interaction continuum(Nancy Johnson & Co)
What conditions influence where on the continuum agiven interaction falls?
Mycorrhizal response (MR) of various plant species at ambient and elevated CO2.
MR > 0 means better growth with AM than without AMMR < 0 means better growth without AM than with AM
0
1
2
3
4
5
6 Agropyron repens
- Nitrogen + Nitrogen
Plant growth is reduced by a full soil community that includes mycorrhizal fungi (filled bars) compared to the partial community (open bars). Added N significantly reduces the stunting of plant
growth by the full soil community.T
ota
l pla
nt m
ass
, g
Full Partial Full Partial Full Partial Full Partial
Soil Community
Are soil organisms competing with plants for nitrogen?
Summary on mycorrhizae
• Symbiosis with mycorrhiza allows greater soil exploration, and increases uptake of nutrients (P, Zn, Cu, N, water)
• Mycorrhiza gets carbon from plant
• Great SA per mass for hyphae vs. roots
• Not all mycorrhizal associations benefit the plant!
• Two main groups of mycorrhiza – Ectomycorrhiza and VA-mycorrhiza
For usmore on nitrogen nutrition
•Why is N so important for plant growth?
•What percentage of the mass of plant tissues is N?
•What kinds of compounds is N found in?
•Why is there a strong relationship between the Nconcentration of leaves and photosynthesis?
Nitrogen - the most limiting soil nutrient
Evidence - factorial fertilization experiments (N, P, K, etc.)
show largest growth response to N.
1. Required in greatest amount of all soil nutrients
2. A component of proteins (enzymes, structural proteins,
chlorophyll, nucleic acids)
3. The primary photosynthetic enzyme, Rubisco, accounts
for a 25 to 50% of leaf N.Photosynthetic capacity is strongly correlated with leaf N concentration.
4. Availability in most soils is low
5. Plants spend a lot of energy on N acquisition - growing
roots, supporting symbionts, uptake into roots, biochemical assimilation into amino acids, etc.
The inorganic forms of nitrogen in soils.
1.NH4+, ammonium ion. A cation that is bound to clays.
2.NO3-, nitrate ion. An anion that is not bound to clays.
Nutrient “mobility” in soils refers to the rate of diffusion,which is influenced by nutrient ion interactions with soil particles.
Would you expect NH4+ or NO3- to diffuse more rapidly?
Would you expect a more pronounced depletion zone for NH4+ or NO3
-?
SoilOrganic N
NH4+
Plant N
NO3-
NH4+ uptake
Mineralization
NH4+
immobilization
Nitrification
NO3-uptake
NO3-
immobilization
NO, N2O
N2
Leaching
Denitrification
N Fixation
Atmospheric N2
The Nitrogen Cycle
Solute Transport (Ch. 6)
1. The need for specialized membrane transport systems.
2. Passive vs. Active Transport
3. Membrane Transport Mechanisms
Fig. 1.4
Why the need for specialized transport systems?
Fig. 1.5A
Fig. 6.6
That the permeability of biological membranes differsfrom that of a simple phospholipid bilayer indicates
that transporters are involved.
Passive vs. Active Transport
Passive transport requires no energy input, G < 0Active transport requires energy, G > 0
Whether active or passive transport is required is determined by the chemical potential of a solute on either side of a membrane.
We can use the G concept to understand the chemical potential.
Hydrostatic
Chemical potential difference
1.Concentration component2.3 RT log (Cj
i/Cjo)
What is the concentration influence on wherethe solute will tend to move spontaneously?
2. Electrical componentzjFE
What is the electrical influence on wherethe solute will tend to move spontaneously?
Movement along electrical and concentration gradients(from Lecture 3)
G = zF Em + 2.3 RT log(C2/C1)
At equilibrium (G = 0) can rearrange this as:
Em = -2.3(RT/zF) log(C2/C1)
Nernst potential•The difference in electrical potential (voltage) between two compartments at equilibrium with respect to a given solute.
•The membrane electrical potential at which the concentration and electrical influences on a solute’s movement are exactly balanced, so there is no net movement.
Ej = 2.3RT (log Cjo/Cj
i) zjF
•At equilibrium, the difference in concentration of an ion between two compartments is balanced by the voltage difference between the compartments.
Cjo Cj
i
Plasmamembrane and the tonoplast are sites of much ion transport.
Fig. 6.4
Maintenance of cell membrane potential requires energy produced by respiratory metabolism
Fig. 6.7
Fig. 6.8
Fig. 6.9
even
Transport of a solute against its concentration gradient can occur by coupling it to proton transport with its concentration gradient.