effect of aflatoxin and toxin binders on milk composition of buffalo
TRANSCRIPT
![Page 1: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/1.jpg)
1
Effect of Aflatoxin and Toxin Binders on Milk Composition of Buffalo Milk
-SAILEE GURAVMSC.BIOCHEMISTRY
PROJECT CO - GUIDEDR. SHAILESH . D. INGOLE
DISSERTATION DONE AT BOMBAY VETERINARY
COLLEGE,PAREL,MUMBAI
![Page 2: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/2.jpg)
2
Introduction Review of Literature Aims and Objective Signification of Research problems Plan of work Material and Methods Result and Discussion Conclusion Bibliography
CONTENT
![Page 3: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/3.jpg)
3
AFLATOXIN
INTRODUCTION
![Page 4: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/4.jpg)
4
AFLATOXIN CONTAMINATION PATHWAY
![Page 5: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/5.jpg)
5
TOXIN BINDERS
BentoniteActivated charcoalZeolite
![Page 6: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/6.jpg)
6
Aflatoxin
Toxin Binder
HOW DOES TOXIN BINDER BINDS AFLATOXIN?
![Page 7: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/7.jpg)
7
CRITERIA FOR SELECTING THE”BEST“ TOXIN BINDER
Toxin binders
![Page 8: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/8.jpg)
8
Michal Hulik et al.,(2014) studied effect of mycotoxin sequestering agents added into feed on health, reproduction and milk yield of dairy cattle.
R. Dokovic et al.,(2011) studied effect of zeolite on the chemical composition of milk from Serbian spotted dairy cattle.
Battacone et al., (2009) studied the transfer of aflatoxin M1 in milk of ewes fed diet naturally contaminated by aflatoxins and effect of inclusion of dried yeast culture in the diet.
REVIEW OF LITERATURE
![Page 9: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/9.jpg)
9
AIM: To evaluate proteins, lactose, fat, SNF in milk of buffalo and milk yield up to 45 days in control and treatment groups fed with and without Toxin Binders.
OBJECTIVES: To compare the levels of fat,lactose,SNF,proteins
in milk of buffaloes and milk yield between control and treatment groups.
AIM AND OBJECTIVES
![Page 10: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/10.jpg)
10
Aflatoxins a major concern
Hepatocellular carcinoma, is the third-leading cause of death
SIGNIFICATION OF RESEARCH PROBLEMS SELECTED
![Page 11: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/11.jpg)
11
In addition to health problems aflatoxins affects nutritional value of milk
Role of toxin binders
Aflatoxins cannot be removed through pasteurization
![Page 12: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/12.jpg)
PLAN OF WORK
![Page 13: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/13.jpg)
MATERIALS
13
48 Murrah Buffaloes
Napier Grass Dry
fodder
CONTROL GROUP
T1Exal + Bentonite
T2Talc
T3Chinal clay
TEST GROUPT1,T2,T3
Collection of Buffalo Milk
samples
Toxin Binders
![Page 14: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/14.jpg)
14
METHODOLOGYLACTOSCAN MILK ANALYZER
![Page 15: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/15.jpg)
15
RESULTS AND DISCUSSIONS
![Page 16: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/16.jpg)
Days C T1 T2 T30 8.97 10.03 7.83 8.767 8.33 8.72 8.56 8.588 9.15 9.4 8.3 8.59 8.97 10.03 7.83 8.76
10 8.58 10.35 8.8 8.5511 8.33 8.72 8.56 8.5812 8.45 8.03 8.22 8.4113 8.56 8.39 7.51 7.9814 8.52 8.63 8.24 8.0315 8.31 8.22 8.12 8.4721 9.5 9.12 9.02 9.5728 11.15 9.76 9.54 9.9635 9.69 9.9 8.6 9.4145 8.52 8.63 8.1 8.03
Means 8.93ª 9.13ª 8.37b 8.68ab
AVERAGE MILK FAT %
a- Sameb-Difference
![Page 17: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/17.jpg)
17
7.8
8
8.2
8.4
8.6
8.8
9
9.2
8.93
9.13
8.37
8.68CONTROLT1T2T3
DAYS
MIL
K FA
T %
T1 T2 T3
GRAPH OF AVERAGE MILK FAT %
CONTROL
![Page 18: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/18.jpg)
AVERAGE MILK PROTEIN %Days C T1 T2 T3
0 4.75 4.75 4.45 4.47 4.55 4.55 4.5 4.48 4.5 4.4 4.65 4.459 3.63 4.09 3.97 3.6
10 3.73 4.35 4.45 4.411 4.05 4.04 4.06 4.0812 4.05 4.08 3.95 3.9713 3.94 4.07 3.97 4.0614 3.88 3.97 4.02 3.9915 3.87 3.51 3.87 3.921 4.1 3.97 3.93 3.9228 4 3.96 3.98 4.1135 3.88 3.99 3.79 3.8145 3.78 3.94 3.68 3.95
Means 4.05 4.11 4.09 4.07
![Page 19: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/19.jpg)
19
4
4.02
4.04
4.06
4.08
4.1
4.12
4.05
4.11
4.09
4.07 CONTROLT1T2T3
DAYS
MIL
K PR
OTE
IN %
GRAPH OF AVERAGE MILK PROTEINS %
CONTROL T1 T2 T3
![Page 20: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/20.jpg)
AVERAGE MILK SNFDays C T1 T2 T3
0 9.82 11.2 10.55 9.817 10.7 10.65 10.7 10.758 10.1 10.3 9.5 10.59 9.82 11.2 9.55 9.76
10 10.15 10.7 10.1 11.211 10.7 10.65 10.7 10.7512 10.7 10.75 10.55 10.5513 11.1 10.7 10.55 10.7514 10.85 10.55 10.65 10.615 10.4 10.65 10.55 10.421 10.55 9.99 10.5 10.7528 11.75 10.5 10.11 11.0835 10.58 10.4 10.01 10.9545 10.35 10.55 10.65 10.23
Means 10.54 10.62 10.33 10.57
![Page 21: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/21.jpg)
21
10.1510.2
10.2510.3
10.3510.4
10.4510.5
10.5510.6
10.65
10.54
10.62
10.33
10.57
CONTROLT1T2T3
DAYS
MIL
K S
NF
% GRAPH OF AVERAGE MILK SNF %
CONTROL
T1 T2 T3
![Page 22: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/22.jpg)
Days C T1 T2 T3
0 6.2 6.6 6.35 6.17 5.7 6.55 6.45 5.758 4.5 5.3 5.7 5.29 4.45 4.5 4.7 5.05
10 4.8 4.5 4.45 5.4511 4.45 4.45 4.35 4.3512 4.45 4.7 4.65 4.413 5.85 5.65 5.6 5.6514 5.75 5.2 5.25 5.3515 5.3 5.9 5.5 5.221 4.2 4.65 4.9 4.9528 4.9 5.75 4.95 4.9535 4.35 4.2 4.45 4.445 4.7 4.55 4.95 4.75
Means 4.97 5.17 5.16 5.11
AVERAGE MILK LACTOSE
![Page 23: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/23.jpg)
23
4.85
4.9
4.95
5
5.05
5.1
5.15
5.2
4.97
5.17 5.16
5.11
CONTROLT1T2T3
DAYS
MIL
K L
ACTO
SE %
GRAPH OF AVERAGE LACTOSE %
CONTROL T1 T3T2
![Page 24: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/24.jpg)
AVERAGE MILK YIELD OF BUFFALOES
![Page 25: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/25.jpg)
25
Day T1 T2 T3 C
AVERAGE MILK YIELD OF BUFFALOES
![Page 26: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/26.jpg)
26
0
2
4
6
8
10
12
8.359.47
11.41
8.62
CONTROLT1T2T3
DAYS
MIL
K YI
ELD
IN
LIT
ERS
AVERAGE MILK YIELD
CONTROL T1 T2 T3
![Page 27: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/27.jpg)
27
CONCLUSION
Lower milk yield in C & T3 groups and higher in T2 & T1groups
Talc and exal + bentonite proved to be best toxin binders
Protein, SNF and lactose concentration did not vary with the treatments
![Page 28: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/28.jpg)
28
BIBLOGRAPHY
E Smith, T D Phillips, J A Ellis, R B Harvey, L F Kubena, J Thompson and G Newton (1994) Dietary Hydrated Sodium Calcium Aluminosilicate Reduction of Aflatoxin M, Residue in Dairy Goat Milk and Effects on Milk Production and Components. J ANIM SCI 1994, 72:677-682.
Battacone, G., A. Nudda, A. Cannas, A. Cappio Borlino, G. Bomboi and G. Pulina (2003) Excretion of aflatoxin M1 in milk of dairy ewes treated with different doses of aflatoxin B1. J. Dairy Sci. 86:2667–2675.
G. Battacone, A. Nudda, M. Palomba, M. Pascale, P. Nicolussi, and G. Pulina (2005).Transfer of Aflatoxin B1 from Feed to Milk and from Milk to Curd and Whey in Dairy Sheep Fed Artificially Contaminated Concentrates. J. Dairy Sci. 88:3063–3069
Razzagh Mahmoudi (2014).Seasonal pattern of aflatoxin M1 contamination in buffalo Milk. Journal of Agroalimentary Processes and Technologies 2014, 20(1), 9-13.
Melkamu Bezabih Yitbarek and Birhan Tamir (2014).Mycotoxines and/or aflatoxines in milk and milk products: Review. International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 4 (10), pp. 294-311.
![Page 29: Effect of aflatoxin and toxin binders on milk composition of buffalo](https://reader035.vdocument.in/reader035/viewer/2022062316/58ef56361a28ab040c8b457b/html5/thumbnails/29.jpg)
THANK YOU