S46 Abstracts / Journal of Biotechnology 185S (2014) S37–S125

mental group, where hatching ability was significantly decreased(20%). The anthelmintic effect of the tested extract may be due totannins presence, to lectin content of the extract or to both. How-ever, the extraction conditions used limited the tannins extractionand lectin should be investigated in future experiments.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.153

In vitro screening of hydroalcoholic plantextracts to control Nosema apis infection

Ion Radoi 1,∗, Agripina Sapcaliu 2, CristinaMateescu 2, Aneta Pop 1, Vasilica Savu 2

1 University of Agronomical Sciences and VeterinaryMedicine Romania, Faculty of Veterinary Medicine,Bucharest, Romania2 Beekeeping Research and Development Institute,Bucharest, Romania

E-mail address: radoiion@yahoo.com (I. Radoi).

Healthy status of bee colonies in Romania, as well as in other Euro-pean countries, is affected by specific parasites and pathogens.Nosema apis and Nosema ceranae are known to be the most infec-tious among European honey bee cultivators. In order to obtaina natural preparation with an antiparasitic effect as an alterna-tive therapy to control Nosema sp. spores development, there havebeen tested 9 hydroalcoholic extracts from different plants andalso propolis extract. The experiments were performed on naturallyinfected honey collected from Romanian apiaries diagnosed withnosemosis, with an estimated of value of 28 spores/field. The infec-tion of the honey samples was determined by OIE 2008 method.The samples were incubated with different plant extracts, foreach extract being performed five replicates. The obtained resultsshowed that Origanum vulgare and Rosmarinus officinalis extracts(with a concentration of 0.7%, g/g, volatile oils) emphasized thehighest antiparasitic action against Nosema sp. After three consecu-tive treatments, the number of spores was significantly reduced, to4 spores/field. These results recommend plant extracts treatmentof honey to control Nosema sp. development, as an alternative toveterinary therapy currently in use.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.154

Proteomic disproportion of nucleoli in pig andmouse fully grown oocytes

Martin Morovic 1,∗, Frantisek Strejcek 1, JozefFulka Jr. 2, Poul Hyttel 3, Jozef Laurincik 1

1 Constantine the Philosopher University, Nitra,Slovak Republic2 Department of Biology of Reproduction, Institute ofAnimal Science, Prague, Czech Republic3 Faculty of Life Sciences, Department of BasicAnimal and Veterinary Sciences, University ofCopenhagen, Copenhagen, Denmark

E-mail address: mmorovic@ukf.sk (M. Morovic).

Nucleoli of somatic cells and growing oocytes are composed offibrillar centers, dense fibrillar components, and granular compo-nents. During the final stage of oocyte growth, the morphology ofnucleoli becomes uniformly fibrillar (nucleolus precursor bodies –NPBs) and the chromatin separates from them. The function of NPBsin oocytes and embryos has been characterized after the inventionof enucleolation. This method represents the removal of nucleolifrom fully grown and growing oocytes. In our study 20 nucle-

oli were isolated from each, porcine and mouse, germinal vesicle(GV) oocytes by micromanipulation for determination of total pro-tein concentration using colloidal gold staining method. The totalprotein concentration in porcine GV oocytes was estimated to beabout 0.4 ng �l−1. In the other hand, mouse GV oocytes showedmore than 20 times higher protein concentration, 8.4 ng �l−1. Thesedifferences were also noticeable during the intergeneric nucleolo-transfer procedure, where porcine nucleoli were insufficient formajor genome activation in mouse embryos. The average cleavagerate of these embryos was less than 2%, and none of them reachedthe blastocyst stage. Porcine embryos with mouse nucleolus werefully capable to develop to final blastocyst stage. For explanationof this intergeneric barrier, further qualitative proteomic analysesare needed.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.155

Effects of confluency, roscovitine and serumstarvation on the cell-cycle synchronization andviability of sheep and goat adult fibroblasts

Aysel Eren 1, Sezen Arat 2,∗, Metin Tuna 2, RıfatBircan 1

1 Namik Kemal University, Faculty of Arts andSciences, Biology Department, Tekirdag, Turkey2 Namik Kemal University, Faculty of Agriculture,Agricultural Biotechnology Deparment, Tekirdag,Turkey

E-mail address: sarat@nku.edu.tr (S. Arat).

The cells in 2–6 passages were randomly allocated into six treatedgroups. Cells were cultured either in culture medium with 30 �Mroscovitine for 24 h (group 1), 15 �M roscovitine for 24 h (group2), in culture medium until 100% confluent (group 3), 100% con-fluent for 3 days (group 4), or in culture medium containing 0.5%FBS for 3 days (group 5), 0,5% FBS for 5 days (group 6). Analysisof cell cycle distribution of goat cell by flow cytometry showedthat the ratios of arrested cells in the G0/G1 phase were higher(p < 0.05) in group 5, 6 (85.84%, 88.68%, respectively) than otherfour groups (73.23%, 71.83% 82.24%, 83.41% group 1, 2, 3, 4). Sameanalysis of sheep cells showed that the ratio of arrested cells in theG0/G1 phase were higher in group 3, 4, 5, 6 (84.62%, 82.63%, 82.79%,82.71%, respectively) than other two groups (51.33%, 66.64%, group1, 2). Although double staining by PI and Annexin V FITC anal-ysis of cell showed that there were no differences in viability ofsheep cells between groups, viability of goat cells reduced in 1, 2,4, 5. This result showed that goat cells are more sensitive thansheeps cells base on cell viability to cell synchronization proto-cols. This research was supported by TUBITAK with grant numbersTOVAG-1120932 and Namik Kemal University with grant numbersNKUBAP.00.24.AR.12.10.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.156