Effects of ESSENTIOM+ on postpartum immune function and subsequent reproductive performance.Pankowski J, Noble J, Brennan P, Jarrett G, Block E. Effects of ruminally inert essential fatty acids on postpartum immune-related functions and productivity in lactating dairy cattle. J Anim Sci 2013;91: (Suppl.2)/J Dairy Sci 2013;96:(Suppl.1) Abstr. 659. Presented at the ADSA Joint Annual Meeting 2013.

Herd Challenge.Two large commercial dairies in New York were identified to demonstrate the effects of ESSENTIOMTM+ relative to postpartum immune function. Working with the dairy nutritionists, ESSENTIOM was introduced into the diet. • Feeding rate: – 0.25 lbs. per head per day 21 days prepartum – 0.75 lbs. per head per day 100

days postpartum – ESSENTIOM was substituted for other supplemental

dietary fat sources – All diets were equal in energy content • Herd makeup:

• Measurement period: – Herd baseline period: December 2010 – August 2011 – ESSENTIOM treatment period: December 2011 –

August 2012 • Benchmarks: – Adjusted first milk yield – First linear somatic cell score (SCS) – Incidence of Early Embryonic Death (EED) up to

35 days postbreeding – Individual cow blood ß-hydroxybutyric acid (BHBA)

at seven days postpartum – 1st service conception rate (CR)

Improved Immune Function. • 12% reduction in 1st linear SCS • 67% reduction in EED • 44% reduction in BHBA

0%

-10%

-20%

-30%

-40%

-50%

-60%

-70%

a=P<0.01

ESSENTIOM RESULTS ON IMMUNE FUNCTION

BHBA (mg/dL)

1st Linear SCS

EED/mo

-44%a

-12%a

-67%a

Greater Reproductive Performance.REPRODUCTIVE PARAMETERS IMPROVED*

Baseline ESSENTIOM P-Value % Increase

Heat Detection rate (%) 66.7 67.7 0.318 +1.4%

Conception Rate — All services(%) 35.6 38.2 0.035 +7.3%

Pregnancy Rate (%) 23.8 25.9 0.034 +8.8%

*1st service conception rates alone increased four points from 35.4% to 39.4% (P=0.08)

Case Study

BHBA Level Significance:

• Peripartum concentrations of circulating BHBA are useful indicators of the ability of cows to deal with metabolic challenges in the transition period.

• Increased concentrations of BHBA at calving have been associated with increased risk of disease1-6 and impaired early reproductive performance.5,7

• Serum concentrations of BHBA measure oxidation of fat and reflect the success of a cow in adapting to negative energy balance.8

NUMBER OF COWS BASELINE ESSENTIOM

Overall 1,989 2,219

Herd A 1,015 1,110

Herd B 974 1,109

+ESSENTIOM is a renaming of MEGALAC®-R. The product has not changed in form or composition.

The results of this on-farm demonstration are consistent with previous research in which animals with increased BHBA around parturition experienced a higher risk of milk loss at the first DHIA and impaired early lactation reproductive performance.11

Increased Milk and Component Yield.Pounds of fat and protein significantly increased in the ESSENTIOM™ treatment groups even though percent fat and protein decreased.

Economic Benefits.Feeding ESSENTIOM on these dairies 21 days pre- to 100 days postpartum translated to multiple economic advantages, and an overall 15:1 return on investment (ROI).

1. Ospina PA, Nydam DV, Stokol T, Overton TR. Evaluation of nonesterified fatty acids and ß-hydroxybutyrate in transition dairy cattle in the northeastern United States: Critical thresholds for prediction of clinical diseases. J Dairy Sci 2010;93:546–554.

2. Chapinal N, Carson M, Duffield TF, Capel M, Godden S, Overton MW, Santos JEP, LeBlanc SJ. The association of serum metabolites with clinical disease during the transition period. J Dairy Sci 2011;94:4897-4903.

3. Seifi HA, LeBlanc SJ, Leslie KE, Duffield TF. Metabolic predictors of post-partum disease and culling risk in dairy cattle. Vet J 2011;188:216–220.

4. Duffield TF, Lissemore KD, McBride BW, Leslie KE. Impact of hyperketonemia in early lactation dairy cows on health and production. J Dairy Sci 2009;92:571–580.

5. Ospina PA, Nydam DV, Stokol T, Overton TR. Associations of elevated nonesterified fatty acids and ß-hydroxybutyrate concentrations with early lactation reproductive performance and milk production in transition dairy cattle in the northeastern United States. J Dairy Sci 2010;93:1596–1603.

6. Chapinal N, Carson M, LeBlanc SJ, Leslie KE, Godden S, Capel M, Santos JEP, Overton MW, Duffield TF. The association of serum metabolites in the transition period with milk production and early-lactation reproductive performance. J Dairy Sci 2012;95:1301–1309.

7. Walsh RB, Walton JS, Kelton DF, LeBlanc SJ, Leslie KE, Duffield TF. The effect of subclinical ketosis in early lactation on reproductive performance of postpartum dairy cows. J Dairy Sci 2007;90:2788–2796.

8. Herdt TH. Ruminant adaptation to negative energy balance. Vet Clin North Am Food Anim Pract 2000;16:215-230.

9. De Vries A. Economic Value of Pregnancy in Dairy Cattle. J Dairy Sci 2006;89:3876–3885.

10. Fricke P, Stewart S, Rapnicki P, Eicker S, Overton M . Pregnant vs. Open: Getting Cows Pregnant and the Money It Makes, in Proceedings. Western Dairy Management Conference 2005;51-53.

11. Chapinal N, LeBlanc SJ, Carson ME, Leslie KE , Godden S , Capel M, Santos JEP. Herd-level association of serum metabolites in the transition period with disease, milk production, and early lactation reproductive performance. J Dairy Sci 2012;95:5676–5682.

© 2017 Church & Dwight Co., Inc. ARM & HAMMER™, ESSENTIOM™ and their logos are registered trademarks of Church & Dwight Co., Inc. MEGALAC® is a registered trademark of Volac International Limited and is licensed to Church & Dwight Co., Inc. ML2662-0217

2.79a

3.5a

SIGNIFICANT INCREASES IN COMPONENT YIELD WITH THE ESSENTIOM TREATMENT GROUPS

% FatLbs Protein/dLbs Fat/d

2.53a

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

2.71

2.27

3.67

2.94a3.09

% Protein

a=P<0.01

Treatment

Baseline

34.7 36.5a

79.8a

SIGNIFICANT INCREASES IN MILK YIELD WITH THE ESSENTIOM TREATMENT GROUPS

FCMMilkAdjusted 1st Milk0

10

20

30

40

50

60

70

8073.7

79.9a

75.7

a=P<0.01

Treatment

Baseline

To learn more about how ESSENTIOM can help improve immune function on your dairy, contact your nutritionist, veterinarian, ARM & HAMMER™ representative or visit AHanimalnutrition.com.

PARAMETER BASELINE ESSENTIOM DIFFERENCES PER COW PER YEAR

Solids Corrected Milk Incomea $4,892 $5,062 $170Cost of Pregnancy Loss (EED)b ($7) ($2) $5Value of Pregnancy Rate (PR)c $0 $12 $12Bypass Fat Cost (Pretreatment vs. ESSENTIOM)d ($64) ($75) ($11)Net Benefit (Ratio) 974 1,109 $176(15:1)

a Solids Corrected Milk Income: Corrected volume (up or down based on 3.5% fat and 3.2% protein) was calculated using MilkPay.com on October 30, 2012.b Cost of Pregnancy Loss: 67% less pregnancy loss (EED) at $207 per loss9 with a 3.4% baseline EED incidence rate.c Value of Pregnancy Rate: 2.1 percentage points improvement valued at $6.00 per point10 with a 24% baseline Pregnancy Rate. Value of Pregnancy Rate improvements will differ depending on individual herd baseline value.d Cost of Pretreatment Bypass Fat: $1600 per ton (October 30, 2012) fed prepartum (21 days at a feeding rate of 0.25 lb. per head per day) and postpartum (100 days at a feeding rate of 0.75 lb. per head per day).

Cost of MEGALAC-R: $1880 per ton (October 30, 2012) fed prepartum (21 days at a feeding rate of 0.25 lb. per head per day) and postpartum (100 days at a feeding rate of 0.75 lb. per head per day).