Ahmed El-Sohemy

Nutrigenomics

Department of Nutritional Sciences

University of Toronto

Canada Research Chair in Nutrigenomics

Nutrigenomix Inc.

Toronto – Sydney – Doha

Chief Science Officer

Diet Genes

Using genomic information and high throughput

‘omics’ technologies to address issues important

to nutrition and health.

Food preferences

Nutrigenomics

Genetic Association Studies

Disease-associated Genes

Modifier Genes

vs

Increase

Decrease

No Effect Genes

Genotype A

Genotype C

Genotype B

Health

Outcome Nutrition

Role of ‘Modifier’ Genes in Nutrition

Increase

Decrease

No Effect Genes

Genotype A

Genotype C

Genotype B

CVD Coffee

Is Coffee associated with CVD?

caffeine

magnesium

potassium

polyphenols

aliphatic acids

diterpenoids

melanoidins

Bioactives in coffee

Caffeine (1,3,7-trimethylxanthine)

“The Legal Alternative”

Caffeine

CYP1A2

1-methylxanthine

1-methyluric acid

5-acetylamino-6-

formylamino-3-

methyluracil

1,7-dimethyluric

acid

Paraxanthine

AA AC CC0

50

100

150

200

250

300

CYP1A2 Genotype

% In

du

cib

ilit

y

slow

fast

Genetic Variation in CYP1A2 -163 AC

• 2013 cases (myocardial infarction)

• 2013 population-based controls - matched (age, sex, area of residence)

• Data collection: - food frequency questionnaire

- health and lifestyle questionnaire

- fasting blood sample (DNA)

Costa Rica Heart Study

0.5

1.0

1.5

2.0<1 cup/d

1 cup/d

2-3 cups/d

4 cups/d

Total Population

Od

ds R

ati

o *

* P<0.05

Cornelis et al., JAMA 295: 1135-41, 2006

Coffee Intake and Risk of Myocardial Infarction

AA AC + CC0.5

1.0

1.5

2.0<1 cup/d

1 cup/d

2-3 cups/d

4 cups/d

CYP1A2 Genotype

Od

ds

Rati

o

* P<0.05

*

*

Cornelis et al., JAMA 295: 1135-41, 2006

Coffee Intake and Risk of Myocardial Infarction

Coffee Intake and Risk of Myocardial Infarction

AA AC + CC0

1

2

3

4<1 cup/d

1 cup/d

2-3 cups/d

4 cups/d

CYP1A2 Genotype

Od

ds

Rati

o

Subjects <50 Years of Age

* P<0.05

*

*

*

*

Cornelis et al., JAMA 295: 1135-41, 2006

Personalized Dietary Advice

Public Health Recommendations

vs

USA, 2007-2012

UK/USA, 2000 - 2009

USA, 2007

USA, 2009

Canada, 2009

Iceland, 2007-2012

USA, 2006

USA, 2007

USA, 2001 USA, 1998

USA, 2000

UK, 2010

DTC Genetic Tests

I have the gene,

so what can I do?

I have the gene,

so I eat healthily.

Does genetic information influence behaviour?

Study Design • Randomized Controlled Trial (NCT 01353014) – clinicaltrials.gov

• Subjects from the Toronto Nutrigenomics and Health Study (n=140) – 20-35 years old – Multi-ethnic population

• Results for 4 genetic markers (CYP1A2, ACE, GSTT1, TAS1R2)

• Follow-up for 3 months and 12 months

Study Groups

General Dietary Recommendations

Targeted Dietary Advice

Intervention Group

Control Group

General Dietary Recommendations

Genotype “A” Non-Risk

Genotype “B” Risk

DNA-based dietary advice resulted in:

greater understanding of recommendations

greater interest in learning more

greater motivation to change eating habits

improved dietary outcomes (to be published)

www.nutrigenomix.com

University of Toronto start-up company

Launched in Canada, June 2012

Available in 180 clinics

Panel of 7 genetic tests

Available exclusively through a dietitian

Provides DNA-based personalized nutrition advice

About Nutrigenomix

Sample Report

Ahmed El-Sohemy, PhD Canada Research Chair in Nutrigenomics University of Toronto

David Castle, PhD Chair of Innovation in the Life Sciences University of Edinburgh

Lynnette R Ferguson, D.Phil., DSc Program Leader of Nutrigenomics New Zealand University of Auckland

J. Bruce German, PhD Director of Foods for Health Institute University of California, Davis

Ben van Ommen, PhD Director of the Nutrigenomics Organisation TNO Quality of Life

Bénédicte Fontaine-Bisson, RD, PhD Assistant Professor in Nutrition Sciences University of Ottawa

Jose Ordovas, PhD Director of Nutritional Genomics Tufts University

David Jenkins, MD, PhD Canada Research Chair in Nutrition and Metabolism University of Toronto

International Science Advisory Board

Starter Package for Dietitians

“…making day-to-day decisions about what to eat based on their particular genetic makeup.”

~1/3 of consumers will focus on personalized nutrition

Daiva Nielsen Leah Cahill Bénédicte Fontaine-Bisson Marilyn Cornelis Ilana Platt Stephen Ozsungur Karen Eny Laura Da Costa

Hyeon-Joo Lee Susana Huang Lindsay Stewart Alejandra Navarro-Allende Nora Khataan Zhen Liu Darren Brenner

Canada Research Chairs

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council

Advanced Foods and Materials Network Centres of Excellence

Alaa Badawi Hannia Campos Tom Wolever David Jenkins Steven Narod Paolo Palatini

Acknowledgements

- Hippocrates (480 BC)

“Positive health requires a knowledge of man’s primary

constitution and of the powers of various foods, both

those natural to them and those resulting from human

skill.”

Personalized Nutrition

Genotype

The International Society of Nutrigenetics and Nutrigenomics invites you to Canada for its 7th annual scientific meeting

to be held in beautiful Québec City!

Discover the latest findings on nutrigenetics and nutrigenomics as they relate to obesity, aging, cancer, diabetes and its cardiometabolic factors, inflammation and oxidative stress as well as translation into nutrition and personalized medicine.

Confirmed guest speakers include: Claude Bouchard, Pennington Biomedical Research Center

Frank Hu, Harvard School of Public Health

Ruth Loos, Charles Bronfman Institute of Personalized Medicine

Charles Serhan, Harvard Institutes of Medicine

Luigi Fontana, Washington University School of Medicine

David M. Mutch, University of Guelph

Ahmed El-Sohemy, University of Toronto

Peter Jones, University of Manitoba

Sign up with our mailing list to receive news updates, first call for abstracts, confirmed speakers and more.

Quebec City Convention Centre 5 to 8 October 2013

Detailed program to come www.isnn2013.org