elf-induced dna breaks
DESCRIPTION
ELF-Induced DNA Breaks. Henry Lai Department of Bioengineering University of Washington Seattle, WA USA. DNA Strand breaks -single and double strand breaks -mis-repair cumulative -affect cell functions; can lead to cancer and cell death. COMET ASSAY. - PowerPoint PPT PresentationTRANSCRIPT
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ELF-Induced DNA Breaks
Henry Lai
Department of Bioengineering
University of Washington
Seattle, WA
USA
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DNA Strand breaks
-single and double strand breaks-mis-repair cumulative-affect cell functions; can lead to
cancer and cell death
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COMET ASSAY
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DNA strand breaks have been reported in different cell types after ELF EMF exposure.
Ahuja (99)- human leukocytesDelimaris (06)- human lymphocytesHong (05)- mouse sperm cellsIvancsit (02, 05)- human fibroblasts and melanocytesIvancsit (03)-more pronounced effect observed in
fibroblasts of older subjects.Jajte (01)- rat lymphocytesLai and Singh (97, 04)- rat brain cellsLi (01)- E. coliSvedenstal (99)- mouse brain cellsWolf (05)- HL-60 and rat fibroblastsZmyslong (00, 04)- rat lymphocytes
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ELF EMF affects DNA repair
Robison (02) HL-60 –decrease
Chow (00) – E. coli- increase- heat shock protein
Schmitz (04)- Mouse brain and kidney cells- increase
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ELF EMF genotoxic effects involve free radicals
Effects blocked by free radical scavengers, enhanced by divalent metals, increase oxidative-damage in molecules
Jaite (01)Lai and Singh (97)Li (01)Lourencini (00)Yokus (05)Wolf (05)Zmyslong (00, 04)
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Electromagnetic fields
iron
mitochondria
H2O2OH
Cellular damage
THE FENTON REACTION
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Intensity and duration of exposure interact.
High intensity/short duration = Low intensity/long duration