elitegro-adv. porduct description v1.0 feb2018 · title: microsoft word - elitegro-adv. porduct...
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Cell Culture Supplement EliteGroTM-Adv. Cat# EPA-500 | EPA-050 Cat# EPAGMP-500 | EPAGMP-050 V1.0 (02/2018)
EliteCell BioMedical Corp. | 206 Archway Drive, Woodway, Texas 76712 | [email protected]
EliteGroTM-Adv. provides abundant human source growth factors and cytokines and works as effective replacement for FBS (fetal bovine serum) to support cell expansion. EliteGroTM-Adv. is animal serum-free, non-xenogeneic cell culture supplement for research or industrial commercial cell growth and proliferation of multiple types of mesenchymal stem cells.
Products Information
Products Catalog No. Spec
EliteGroTM-Adv. EPA-500 500 ml
EliteGroTM-Adv. EPA-050 50 ml
EliteGroTM-Adv. GMP Grade EPAGMP-500 500 ml
EliteGroTM-Adv. GMP Grade EPAGMP-050 50 ml
Products Storage
EliteGroTM-Adv. products are most stable when stored frozen (-20°C or below). Products have 3.5 years shelf life determined from Date of Manufacture, continuously stored frozen (-20°C or below) in original bottle. The manufacture date is indicated on the product label.
Completed media containing EliteGroTM-Adv. can be stored at 2 °C to 8 °C for maximum of 2 weeks.
Once EliteGroTM-Adv. products are thawed, it is recommended to use in completed medium preparation (e.g. 5 %) immediately, or to divide it into single-use aliquot and store at -20 °C.
NOTE: Avoid repeated freeze-thaw cycles as it will cause potential decrease in EliteGroTM-Adv. performance and an increase in insoluble particles.
Applications
EliteGroTM-Adv. is designed to promote cell growth and replace animal serum (e.g. FBS) in multiple cells culture media. For human ex-vivo tissue and cell culture processing applications.
Suggested Cell Type
Bone marrow msenchymal stem cell Adipose tissue derived msenchymal stem cell Umbilical cord derived msenchymal stem cell Other kinds of msenchymal stem cell
Other supplies required
• Typical cell culture basal media. (MSC-qualified basal medium is recommended)
Culture Medium Preparation
Thaw frozen EliteGroTM-Adv. products at 37 °C before use.
Prepare completed media by adding EliteGroTM-Adv. as supplement to 5% (v/v), addition of Heparin is NOT required.
Seed MSCs at approximately 3×103 ~ 6×103
cells/cm2 (recommended cell concentration).
Precipitation in Cell Culture
Clotting or insoluble particles may form in thawed EliteGroTM-Adv. Experiments have showed that particles have no impact to the performance of the products.
It is recommended to centrifuge at 3,400 ×g for 3 to 5 minutes or to filter the liquid with a sterile 40 μm Cell Strainer to remove insoluble particles.
If required by user's system, filtering the completed media after adding 5% EliteGroTM-Adv. by 0.22 μm filters.
NOTE: 0.22 μm filtering is NOT recommended for EliteGroTM-Adv 100 % concentrate as it may cause decrease in performance.
Cell Culture Supplement EliteGroTM-Adv. Cat# EPA-500 | EPA-050 Cat# EPAGMP-500 | EPAGMP-050 V1.0 (02/2018)
EliteCell BioMedical Corp. | 206 Archway Drive, Woodway, Texas 76712 | [email protected]
Safety Information
• EliteGroTM-Adv. products are tested for microbial, endotoxin, and mycoplasma. Each lot is human MSC-qualified.
• EliteGroTM-Adv. products contain human original materials which are non-reactive to various infectious disease according to FDA and AABB requirement.
• EliteGroTM-Adv. are produced manufactured at our facility in a clean room environment which meets ISO 14644 requirement and is 21 CFR Parts 820;; Current Good Manufacturing Practice (cGMP) compliant.
• Handle EliteGroTM-Adv. products in accordance with bio-safety practices, wear appropriate protective eyewear, clothing, and gloves.
Availability • Sample aliquots are available for your testing • Lot reservation are available
References
Peak serum: implications of serum supply for cell therapy manufacturing Regen. Med. (2012) 7(1), 7-13
Shanbhag S, Stavropoulos A, Suliman S, Hervig T, Mustafa K.Efficacy of Humanized Mesenchymal Stem Cell Cultures for Bone Tissue Engineering: A Systematic Review with a Focus on Platelet Derivatives. Tissue Eng Part B Rev. 2017 Dec;;23(6):552-569.
Hemeda H, Giebel B, Wagner W. Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells. Cytotherapy. 2014 Feb;;16(2):170-80.
Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, van Hinsbergh VW, Kamp O, Helder MN, Musters RJ, van Dijk A, Juffermans LJ. Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications. Cell Tissue Res. 2012 Apr;;348(1):119-30.
Bieback K, Hecker A, Kocaömer A, Lannert H, Schallmoser K, Strunk D, Klüter H. Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells. 2009 Sep;;27(9):2331-41.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, ELITECELL BIOMEDICAL AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.