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BIOLOGIA MOLECULAR NO SUPERMERCADO
Francisco J. L. Aragão
Embrapa Recursos Genéticos e Biotecnologia
Development of transgenic technologies: generation of the first commercial transgenic
varieties in Brazil
Francisco J. L. AragãoEmbrapa/Cenargen, Brazil
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Cotton
Genetic Resources and Biotechnology (1986)
Bean and Rice
Soybean
Corn and Sorghum
cVegetables
42 Research Units 2,500 PhD researchers
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1986 – Agricultural Biotechnology Program
Biotechnology Unit – Brasília
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LettuceCottonDry BeanSoybeanPotatoBananaPapayaBrachiariaTomatoCoffeeMaizeCastor BeanCowpea
c
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1996 – Embrapa/BASF – Development of a Production system (Cultivance®) for soybean
BASF
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1996 - Production system Cultivance®2003 – Second Elite Event (CV127)
Microspores
Mendelian segregation3:11 2 3 4
1. Non-transgenic 2. Transgenic (line 8-19) after pulverization with the herbicide3. Transgenic (line 7-15) after pulverization with the herbicide4. Non-transgenic plant after pulverization with the herbicide
BASFB
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Imidazolinone tolerant soybean – Embrapa/BASF
BASF
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Biosafety: safe or safer
Biosafety analisys by Embrapa and BPS
BASF
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Molecular characterization
• Number of DNA inserts, insert stability• Number of copies of genetic elements
within the insert• Integrity of gene cassettes• Presence of additional DNA (backbone)• Sequence of genomic flanking DNA• Sequence of the inserted DNA• DNA LandMarks
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Protein characterization,Food / Feed safety
•Protein characterization and equivalence•Protein level in plant tissues•Gram quantity protein production, purification, characterization•Allergenicity assessment•Toxicity assessment
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Agronomic equivalence, Environmental safety
•Agronomic / phenotypic assessments•Weediness assessment (HT)•Fitness•Environmental safety•Environmental fate
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Composition equivalence,Nutritional equivalence
•Grain samples from multi-location, multi-year, replicated field trials•Secondary metabolites•Pilot scale processing, nutrient / antinutrient analysis (validated methods)•Confirmation of food/feed safety•Animal feeding studies
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Soybean CV127
BASF
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Biosafety: safe or safer
Dec, 2008BASF
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Commercial release proposal
Submitted: USA, Canada, EU, Japan, China, Philippines, Korea and Brazil (Dec 2009)
Submission in 2010: Argentina, Colombia, Taiwan, Australia, Russia, Mexico
BASF
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Golden mosaic causes annual reductions in the range of 90,000 to 280,000 tons (Brazil)Enough to feed 6 M to 20 M adults.
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BGYMVBGMV
> feijão das águasBGYMV-DR, -MX (83%), -FL 82% 44 bp sequence with no gaps
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Genetic Resources and Biotechnology
Agrobiology
Common beanF5.1
Rice and Bean
Maize and Shorgum
Soybean
Agroindustry
UNESP-Botucatu
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Thank youGracias