embryo 090423111342-phpapp02
TRANSCRIPT
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Embryo Transferin Beef Cattle
Ms. BailesAGSC 336 – Advanced Animal Science
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Objectives
• Define embryo transfer
• Explain the steps of embryo transfer
• List the advantages of embryo transfer
• List the disadvantages of embryo transfer
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What is Embryo Transfer?
Collecting embryos from asuperovulated donor cow...
…and transferring the embryos to synchronized recipients to complete the gestation period.
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The Donor Cow
• Top quality female, free of reproductive abnormalities or genetic defects, proven maternal qualities.
• Personal goals/preferences play large part in donor selection.
• Marketability of the donors calves.
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Superovulation of DonorDay 0 to Day 4
• Follicle Stimulating Hormones (Known as FSH)
• Injections 2x daily, 4 days
(Day 0 starts 8-14 days following estrus cycle.)
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Result: Multiple Folicles
Superovulation--Day 3
• Prostaglandin injection causing Corpus Luteum regression to bring on estrus.
Result: Multiple Folicles
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Superovulation--Day 5
• Onset of estrus
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A.I. of Superovulated Donor• Artificially Inseminate when in
standing estrus, usually 5 days after superovulation.
• Usually multiple inseminations at 12, 24, 36 hrs. after onset of estrus.
• Preferably, more than one straw of high quality semen.
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Embryo Recovery from Donor
• Approximately 7 days after breeding.
• Easy, takes less than 30 minutes.
• Insert foley catheter with inflatable balloon into donor’s uterus.
• Flushing solution (saline) is introduced into each uterine horn.
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Recovery of Embryos
• Solution filled uterine horn is gently massaged.
• Fluid containing the embryos is drawn back out and collected though a filter into a holding cylinder.
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Embryo Processing• After 20-30 minutes embryos
located with stereoscopic microscope.
• Embryos are washed and transferred to holding medium. (Procedure generally repeated 3 times.)
• Embryos are evaluated for state of development and quality.
• Initially classified: “good” or “bad.” “Good” embryos are further classified.
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The Recipient
• Reproductively sound, good mothering ability.
• Good overall health, nutrition.
• Must be synchronized to receive the embryo.
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Synchronization Protocol--Day 0
• Insert intravaginal CIDR.
• Administer 2 cc combo Estradiol Benzoate and Progesterone.
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Day 7
• Remove CIDR
• Administer 5cc Prostaglandin
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Day 8
• Final Injection:
1 cc Estradiol Benzoate
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Day 8 through Day 10
• Day 8 pm through Day 10am:
Observe for onset of estrus.
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Day 16--Transfer Embryo
• Transfer Embryo into synchronized recipient who has come into estrus.
• Procedure is simple,
much like A.I.
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Activity
• Each card has a step in the embryo transfer process.
• Put yourselves in the proper order of embryo transfer and then explain the process.
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Advantages of E.T.
• Increased number of calves of genetically
superior cows.
• Increased marketing opportunities--
offspring AND embryos.
• Ease of Import/Export.
• Embyros can be stored indefinitely.
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Disadvantages of E.T.
• Increased expenses and higher break-even costs for
calves.
• Estrus detection required.
• Synchronization of recipient with donor.
• Specialized equipment and trained personnel.
• More expensive and time consuming than
traditional reproductive methods.
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In Summary…
• E.T. is not for every herd.
• Each female born has thousands of potential eggs.
• Complete the crossword puzzle which is a summary/review of what we learned today.
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