1. Engineering Aphid Symbionts for Food Security

Kate Elston

Microbiology, University of Texas at Austin, Austin, TX

2. Managing Data with the Experiment Data Depot

William Morrell1, Mark Forrer2, Garrett Birkel3, Nathan J Hillson4, Hector Garcia-

Martin5, Teresa Lopez6, Tyler Backman7, Chris Petzold8, Edward E. K. Baidoo9, David

Ando10, Zak Costello11, and Ian Vaino12

(1)Biomass Science & Conversion Technologies, Sandia National Laboratory,

Livermore, CA, (2)Biomass Science & Conversion Technology, Sandia National Lab,

Emeryville, CA, (3)Physical Biosciences, Lawrence Berkeley National Laboratory,

Berkeley, CA, (4)Technology Department, DOE Joint BioEnergy Institute,

EMERYVILLE, CA, (5)Biofuels and Bioproducts, Joint BioEnergy Institute, Emeryville,

CA, (6)Sandia National Lab, Emeryville, CA, (7)Joint BioEnergy Institute, Emeryville,

CA, (8)Lawrence Berkeley Nation Lab, Emeryville, CA, (9)Biological Systems and

Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA,

(10)Lawrence Berkeley National Lab, Emeryville, CA, (11)U.S. DOE Agile BioFoundry,

Emeryville, CA, (12)Lawrence Berkeley Nation Lab, Berkeley, CA

3. Development of Cell-Free Protein Expression Biosensors for Detection of Human

Performance Biomarkers

Kathryn Beabout1,2, Amy M. Ehrenworth Breedon1,2, Vaughn Litteral2,3, Svetlana

Harbaugh1, Roland Saldanha3,4, Michael Goodson1, Nancy Kelley-Loughnane5, and

Jorge L. Chávez1

(1)711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson

Air Force Base, OH, (2)Integrative Health and Performance Sciences, UES, Inc.,

Dayton, OH, (3)U.S. Air Force School of Aerospace Medicine, Air Force Research

Laboratory, Wright-Patterson Air Force Base, OH, (4)SSI Inc., assigned to U.S. Air

Force School of Aerospace Medicine, Air Force Research Laboratory, Wright-Patterson

Air Force Base, OH, (5)Materials and Manufacturing Directorate, Air Force Research

Laboratory, Wright-Patterson Air Force Base, OH

4. New genetic tools for DOPA incorporation enable catechol-metalloprotein

engineering

Simon d'Oelsnitz Molecular Biosciences, University of Texas at Austin, Austin, TX

5. Directed Evolution of Antimutator Cells to Stabilize Synthetic Biology Against

Evolution

Dacia Leon, Daniel E. Deatherage, Jenna C. McGuffey, Simon D'Alton, and Jeffrey E.

Barrick Molecular Biosciences, The University of Texas at Austin, Austin, TX

6. Rational Design and Optimization of Ten Orthogonal and Modular Cell-Cell

Communication Systems

Chunbo Lou Institute of Microbiology, Chinese Academy of Science, Beijing, China

7. Harnessing Tardigrade-Specific Intrinsically Disordered Proteins to Enhance the

Robustness of Living Medicines

Danny Collins1, Jonathan Stokes1, Luis R. Soenksen2,3, and James J. Collins4,5

(1)Infectious Disease & Microbiome Program, Broad Institute, Cambridge, MA,

(2)Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA,

(3)Living Celular Devices, Wyss Institute at Harvard, Boston, MA, (4)Biological

Engineering, Massachusetts Institute of Technology, Cambridge, MA, (5)Wyss Institute

for Biologically Inspired Engineering, Harvard University, Boston, MA

8. Phase Space Analysis for Gene Circuit Design

Tim Rudge1,2, Macarena A. Muñoz Silva3, Tamara Matute4, Isaac Nuñez5, Carlos A.

Ruiz6, Ambrosio Valdés6, Gonzalo A. Vidal-Peña1, and Fernan Federici7

(1)Institute for Biological and Medical Engineering, Schools of Engineering, Biology and

Medicine, Pontificial Catholic University of Chile, Santiago, Chile, (2)Department of

Chemical and Bioprocess Engineering, School of Engineering, Pontificial Catholic

University of Chile, Santiago, Chile, (3)School of Life Sciencies, Andrés Bello University,

Santiago, Chile, (4)Pontificia Universidad Católica de Chile, Santiago, Chile,

(5)Engineering, Pontificia Universidad Catolica de Chile, Santiago, Chile, (6)School of

engineering, Pontificial Catholic University of Chile, Santiago, Chile, (7)School of

Biological Sciences, Pontificial Catholic University of Chile, Santiago, Chile

9. A Simple, Robust, and Low-Cost Method to Produce the Pure Cell-Free System

Barbora Lavickova1 and Sebastian J. Maerkl2

(1)Institute of Bioengineering,School of Engineering, École polytechnique fédérale de

Lausanne, Lausanne, Switzerland, (2)Institute of Bioengineering, School of Engineering,

Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland

10. Controlling Heterogeneity and Increasing Titer from Riboswitch-Regulated Bacillus

Subtilis spores for Time-Delayed Protein Expression Applications

Denis Tamiev1, Grace Vezeau2, Howard M. Salis3, and Nigel Reuel4

(1)Iowa State University, Ames, IA, (2)Pennsylvania State University, University Park,

PA, (3)Department of Chemical Engineering / Biological Engineering, Pennsylvania

State University, University Park, PA, (4)Chemical and Biological Engineering, Iowa

State University, Ames, IA

11. Scalable Cell-Free Extract Preparation and Minimal Genetic Template Methods for

Rapid Protein Prototyping

Jared Dopp1 and Nigel Reuel2

(1)Iowa State University, Ames, IA, (2)Chemical and Biological Engineering, Iowa State

University, Ames, IA

12. Engineering of Crassulacean Acid Metabolism (CAM) Pathway to Enhance

Photosynthesis and Abiotic Stress Tolerance in C3 Plants

Xiaohan Yang, Degao Liu, Rongbin Hu, and Gerald A. Tuskan

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN

13. 3-D Multicellular Co-Culture Model As a Platform for Rapid Screening of

Engineered Microbial Therapies

Tetsuhiro Harimoto1, Zakary Singer1, Oscar Velazquez1, Joanna Zhang1, Samuel

Castro1, Taylor Hinchliffe1, William Mather2, and Tal Danino1

(1)Biomedical Engineering, Columbia University, New York, NY, (2)University of

California, San Diego, San Diego, CA

14. Capturing Provenance of Designing and Building Biological Systems

John Meng1, Ernst Oberortner1, Nathan J Hillson2, and Jan-Fang Cheng1

(1)DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek,

CA, (2)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA

15. Development of an Optogenetically-Controlled Recombinase for E. coli

Michael Sheets1 and Mary J. Dunlop2

(1)Biomedical Engineering, Boston University, Boston, MA, (2)Department of

Biomedical Engineering, Boston University, Boston, MA

16. Building an Orthogonal Initiation System in a Genomically Recoded Organism

Russel M. Vincent, Bradley M. Wright, and Paul Jaschke

Molecular Sciences, Macquarie University, Sydney, NSW, Australia

17. Building Fungal Factories That Generate Potent Insecticides

Rosannah Cameron1, Kyle Van de Bittner1, Leyla Bustamante1, Rudranuj Bundela1,

Craig van Dolleweerd2, Sarah Kessans3, Arvina Ram4, Barry Scott4, Mathew Nicholson5,

and Emily Parker1

(1)Ferrier Research Institute, Victoria University of Wellington, Wellington, New

Zealand, (2)Protein Science & Engineering, Callaghan Innovation, University of

Canterbury, Christchurch, New Zealand, (3)School of Biological Sciences, University of

Canterbury, Christchurch, New Zealand, (4)Institute of Fundamental Sciences, Massey

University, Palmerston North, New Zealand, (5)Viclink, Victoria University of

Wellington, Wellington, New Zealand

18. Control of Bacterial Communities Using Deep Reinforcement Learning

Neythen J. Treloar1 and Chris P. Barnes2

(1)Cell and Developmental Biology, University College London, London, United

Kingdom, (2)UCL Genetics Institute, University College London, London, United

Kingdom

19. Improving Sakuranetin Biosynthesis Using Metabolically Engineered Microbial Co-

Cultures

Xiaonan Wang, Lizelle Policarpio, and Haoran Zhang

Chemical and Biochemical Engineering, Rutgers University, Piscataway, NJ

20. Retrosynthesis of All Available Pathways to Microbial Production of Precursors to

Target Chemicals Based on Chemical Separation Characteristics

Leanne Whitmore1, Anthe George2, and Corey Hudson1

(1)Systems Biology, Sandia National Laboratories, Livermore, CA, (2)Biomass Science

& Conversion Technology, Sandia National Laboratories, Livermore, CA

21. Controlling Protein Expression Levels in Synthetic, Polycistronic Operon Systems

Daniel Gerngross and Sven Panke

Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland

22. Genetic Code Expansion in Bacillus Subtilis

Devon Stork1, Erkin Kuru1, Aditya M. Kunjapur1, Ethan Garner2, and George M

Church3

(1)Department of Genetics, Harvard Medical School, Boston, MA, (2)Harvard

University, Cambridge, MA, (3)Genetics, Harvard Medical School, Boston, MA

23. Synthetic Genomic Contexts in Bacteria for Extreme Expression Properties

Scott Scholz1, Rucheng Diao1, Michael Wolfe1, Elayne Fivenson2, Xiaoxia (Nina) Lin3,

and Peter Freddolino1

(1)Biological Chemistry, University of Michigan, Ann Arbor, MI, (2)Harvard Medical

School, Boston, MA, (3)Chemical Engineering, University of Michigan, Ann Arbor, MI

24. Leveraging a Yeast Display-Based Stop Codon Suppression Reporter for Rapid

Screening of Aminoacyl-tRNA Synthetases

James A. Van Deventer and Jessica T. Stieglitz

Chemical and Biological Engineering, Tufts University, Medford, MA

25. A Broadly Applicable and Integrated Biological Strategy to Induce Ammonia

Excretion from Symbiotic Diazotrophs for Plant Fertilization

Tim Schnabel1 and Elizabeth Sattely2

(1)Department of Bioengineering, Stanford University, Stanford, CA, (2)Department of

Chemical Engineering, Stanford University, Stanford, CA

26. Characterization of the Met25 As a Facile, Color-Associated, and Counter-

Selectable Genetic Marker in Yarrowia Lipolytica

Harley Edwards1 and Peng Xu2

(1)CBEE, University of Maryland, Baltimore County, Baltimore, MD, (2)Chemical,

Biochemical and Environmental Engineering, University of Maryland, Baltimore County,

Baltimore, MD

27. Developing Computational Design Tools for RNA-Based Gene Regulatory Elements

Calvin M. Schmidt and Christina D. Smolke

Stanford University, Stanford, CA

28. Natural-Vanillin Fermentation from Sugar : Production on Industrial Scale

Jun Takakura1, Keita Fukui1, Miku Matsuzawa1, Sayaka Asari1, Risa Yasuda1, Keiko

Noguchi1, Junko Ito1, Keiko Danjo1, Benjamin Mijts2, Christine Roche2, Sheetal Modi2,

Michael Siddiqui2, Peter Kelly2, Matt Davis2, and Rekha Murali2

(1)Ajinomoto, Kawasaki, Japan, (2)Zymergen, Emeyville, CA

29. Nucleic Acid Cleavage with a Hyperthermophilic Cas9 from an Unculturable

Ignavibacterium

Stephanie Tzouanas Schmidt1, Feiqiao Brian Yu1,2,3, Paul C. Blainey4,5, Andrew P.

May3, and Stephen R. Quake1,3,6

(1)Bioengineering, Stanford University, Stanford, CA, (2)Electrical Engineering,

Stanford University, Stanford, CA, (3)Chan Zuckerberg Biohub, San Francisco, CA,

(4)Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA,

(5)Broad Institute of Harvard and MIT, Cambridge, MA, (6)Applied Physics, Stanford

University, Stanford, CA

30. A Fusion Method to Develop an Expanded Artificial Genomic RNA Replicable By

Qbeta Replicase

Kensuke Ueda1, Ryo Mizuuchi2, Fumio Matsuda1, and Norikazu Ichihashi3,4

(1)Department of Bioinformatic Engineering, Graduate School of Information Science

and Technology, Osaka University, Suita, Osaka, Japan, (2)Department of Chemistry,

Portland State University, Portland, OR, (3)Department of Life Science, Graduate School

of Arts and Science, The University of Tokyo, Meguro, Tokyo, Japan, (4)Komaba

Institute for Science, The University of Tokyo, Meguro, Tokyo, Japan

31. An Efficient Platform for Standardized Genome Engineering in Bacteria

Carolyn Bayer1, Maja Rennig1,2, Anja Ehrmann1, and Morten H.H. Nørholm1,2,3

(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark, (3)CloneOpt

AB, Upplands Väsby, Sweden

32. Using Triplex-Forming Oligos (TFOs) to Repress Promoter Activity in Chinese

Hamster Ovary (CHO) Cells

Mohamed K. Hussein1,2, Martina Baumann2, Heena Dhiman2, Ly Nguyen2, Neža Novak2,

Sybille Galosy3, and Nicole Borth1

(1)Department of Biotechnology, University of Natural Resources and Life Sciences,

Vienna, Austria, (2)ACIB, Austrian Centre of Industrial Biotechnology, Vienna, Austria,

(3)GSK, GlaxoSmithKline, King of Prussia, PA

33. Engineered Mammalian Bioluminescence Enzymes - the First Sensitive Glowing

Mouse Reporter Gene for Biomedicine

James A. Long, Amit P. Jathoul, and James A. H. Murray

BIOSI, Cardiff University, Cardiff, United Kingdom

34. A Synthetic Live Bacterial Therapeutic Organism for the Treatment of the Human

Metabolic Disease Phenylketonuria (PKU)

Vincent Isabella1, David Lubkowicz1, Sarah Rowe1, Binh Ha1, Mary Castillo1, Cami

Anderson1, Paul Miller1, Marja Puurrunen2, and Caroline Kurtz1

(1)Synlogic Inc., Cambridge, MA, (2)Synlogic, Cambridge, MA

35. Synthetic Sequence Entanglement Augments Stability and Containment of Genetic

Information in Cells

Hsing-I Ho, Tomasz Blazejewski, and Harris Wang

Department of Systems Biology, Columbia University, New York, NY

36. Synthetic Symbiosis

Can Huang1, Jasmine Olivares1, Han Wang1, Gerardo Amores2, Paul de Figueiredo2,

and Arum Han1

(1)Texas A&M University, College Station, TX, (2)Texas A&M Health Science Center,

Bryan, TX

37. Programmable Gene Activation in Bacteria

Jennifer Fang1, Hsing-I Ho2, and Harris Wang2

(1)Department of Biological Sciences, Columbia University, New York, NY,

(2)Department of Systems Biology, Columbia University, New York, NY

38. Learning Protein Structure-Function Relationships from Data

Zhiyuan Duan Department of Biochemistry, University of Wisconsin Madison, Madison, WI

39. Genomic Deoxyxylulose Phosphate Reductoisomerase (DXR) Mutations Conferring

Resistance to the Antimalarial Drug Fosmidomycin in E. coli

Gur Pines1, Eun Joong Oh1, Marcelo Bassalo1,2, Alaksh Choudhury1, Andrew Garst3,

Reilly Fankhauser1, Carrie Eckert4,5, and Ryan T. Gill1

(1)University of Colorado Boulder, Boulder, CO, (2)Massachusetts Institute of

Technology, Cambridge, MA, (3)Inscripta, Boulder, CO, (4)Renewable and Sustainable

Energy Institute (RASEI), University of Colorado, Boulder, CO, (5)National Renewable

Energy Laboratory (NREL), Golden, CO

40. A Synthetic RNA-Mediated Evolution System in Yeast

Emil Damgaard Jensen1, Tadas Jakociunas2, Michael K. Jensen3, and Jay Keasling4

(1)The Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kgs. Lyngby, Denmark, (2)Technical University of Denmark, The Novo

Nordisk Foundation Center for Biosustainability, Kgs. Lyngby, Denmark, (3)Novo

Nordisk Foundation Center for Biosustainability, Technical University of Denmark,

Kongens Lyngby, Denmark, (4)Biological Systems and Engineering Division, Lawrence

Berkeley National Laboratory, Emeryville, CA

41. Expanding Boundaries of Synthetic Biology: Discovery and High-Throughput

Characterisation of Inducible Gene Expression Systems

Erik Hanko1, Ana Paiva2, Nigel Minton3, and Naglis Malys1

(1)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences,

University of Nottingham, Nottingham, United Kingdom, (2)Centre for Biomolecular

Sciences, School of Life Sciences, University of Nottingham, Nottingham, United

Kingdom, (3)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences,

The University of Nottingham, Nottingham, United Kingdom

42. Measuring the Efficiency of CRISPR Genome-Editing Systems with Single-Cell

DNA Analysis

Keith W. Jones, Mani Manivannan, Shu Wang, Pedro Mendez, and David Ruff

Mission Bio, South San Francisco, CA

43. Multiplex Transcriptional Characterizations across Diverse Bacterial Species Using

Cell-Free Systems

Sung Sun Yim1, Nathan Johns2, and Harris Wang3

(1)Systems biology, Columbia University, New York, NY, (2)Systems Biology, Columbia

University Medical Center, New York, NY, (3)Department of Systems Biology, Columbia

University, New York, NY

44. Predictable Tuning of Synthetic Microbial Consortium Pulse Generator Dynamics

David Zong1, Mehdi Sadeghpour2, William Ott2, Krešimir Josić2, and Matthew R.

Bennett3

(1)PhD Program in Systems Synthetic and Physical Biology, Rice University, Houston,

TX, (2)Department of Mathematics, University of Houston, Houston, TX, (3)Department

of Bioengineering, Rice University, Houston, TX

45. Bere: Bayesian Quality-Estimator for Reproducible Biological Experiments

Shuowei Li1 and Eric Klavins2

(1)Electrical and Computer Engineering, University of Washigton, Seattle, WA,

(2)Electrical and Computer Engineering, University of Washington, Seattle, WA

46. Single Cell Chemical Imaging with Stimulated Raman Scattering for Biofuel

Production Screening

Nathan Tague1, Jean-Baptiste Lugagne1, Haonan Lin1, Fengyuan Deng1, Wilson Wong1,

Ji-Xin Cheng1, and Mary J. Dunlop2

(1)Biomedical Engineering, Boston University, Boston, MA, (2)Department of

Biomedical Engineering, Boston University, Boston, MA

47. Towards Life with Fewer Than 20 Amino Acids

Liyuan Liu, Tomasz Blazejewski, Christian Munck, Florencia Velez-Cortes, and Harris

Wang

Department of Systems Biology, Columbia University, New York, NY

48. Design and Application of S-Adenosylmethionine Methyltransferase Growth-

Coupling for Evolutionary Engineering

Anne Sofie L. Hansen1, Jakob Hjort Schmidt1, Denis Shepelin1, Lei Yang1, Mette

Kristensen1, Mohammad Radi1, Hanne B. Christensen1, Adam Feist1,2, Jay D.

Keasling1,3,4,5,6, Bernhard O. Palsson1,2,7, Michael K. Jensen1, Markus J. Herrgård1, and

Hao Luo1

(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kongens Lyngby, Denmark, (2)Department of Bioengineering, University of

California - San Diego, La Jolla, CA, (3)Joint BioEnergy Institute (JBEI), Emeryville,

CA, (4)Biological Systems & Engineering Division, Lawrence Berkeley National

Laboratory, Berkeley, CA, (5)Center for Synthetic Biochemistry, Institute for Synthetic

Biology, Shenzhen Institutes of Advanced Technologies, Shenzhen, China, (6)Department

of Chemical and Biomolecular Engineering & Department of Bioengineering, University

of California, Berkeley, CA, (7)Department of Pediatrics, University of California - San

Diego, La Jolla, CA

49. Expression of RNA Origami Scaffolds in Saccharomyces Cerevisiae for

Transcriptional Control

Georgios Pothoulakis, Guido Grossi, and Ebbe S. Andersen

Interdisciplinary Nanoscience Center and Department of Molecular Biology and

Genetics, Aarhus University, Aarhus, Denmark

50. Cytosolic Folding Biosensors for Discovery of Abeta42 and Alpha-Synuclein Folding

Factors in Saccharomyces Cerevisiae

Veronika Sachsenhauser1, Hyun-hee Kim2, Xiexiong Deng2, and James Bardwell2

(1)Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor,

Ann Arbor, MI, (2)Molecular, Cellular, and Developmental Biology, HHMI, University

of Michigan, Ann Arbor, MI

51. Metabolic Engineering of Oleaginous Yeast Yarrowia Lipolytica for Production of

Flavor Lactones

Eko Roy Marella1, Jonathan Dahlin2, Marie I. Dam1, Carina Holkenbrink1, Guokun

Wang2, and Irina Borodina1

(1)The Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kongens Lyngby, Denmark, (2)The Novo Nordisk Center for Biosustainability,

Technical University of Denmark, Kongens Lyngby, Denmark

52. Challenging the Workhorse: Comparative Analysis of Eukaryotic Microorganisms

for Expressing Monoclonal Antibodies

Hanxiao Jiang1, Andrew Horwitz1, Chapman Wright2, Anna Tai1, Elizabeth

Znameroski1, Yoseph Tsegaye1, Hailley Warbington1, Benjamin Bower1, Christina

Alves2, Carl Co2, Kanvasri Jonnalagadda2, Darren Platt1, Jessica Walter1, Venkatesh

Natarajan2, Jeff Ubersax1, Joel R. Cherry1, and J. Christopher Love3

(1)Amyris, Emeryville, CA, (2)Biogen, Cambridge, MA, (3)Department of Chemical

Engineering, The David H. Koch Institute for Integrative Cancer Research,

Massachusetts Institute of Technology, Cambridge, MA

53. The Development of Leucine Consuming Strains As Therapeutics for Maple Syrup

Urine Disease

JR Gao1, Ning LI2, Chris Bergeron1, Lauren Renaud1, Mylene Perreault1, Pat

Cantarella1, Mike James1, Mary Castillo2, David Lubkowicz2, John Thomas1, Paul

Miller2, Caroline Kurtz2, Alex Tucker3, Alex Carlin3, Silvia Galvan3, Ryan Putman3,

Kolea Zimmerman3, Rishi Jain3, and Scott Marr3

(1)Synlogic, Cambridge, MA, (2)Synlogic Inc., Cambridge, MA, (3)Ginkgo, Boston, MA

54. An Electrofermentation Platform for Diterpene Production

Emily Lanier, Michaela TerAvest, and Björn Hamberger

Biochemistry & Molecular Biology, Michigan State University, Lansing, MI

55. Structural and Sequential Analysis Comparing Residues between Functionally

Divergent Terpene Synthases in Prunella vulgaris

Garret P. Miller, Sean R. Johnson, Wajid Waheed Bhat, Radin Sadre, Alekzander Sky

Garcia, and Björn Hamberger

Biochemistry & Molecular Biology, Michigan State University, Lansing, MI

56. Patterns of Microbial Colonization in the Murine Gut Reveals Design Rules for

Robust Microbial Engraftment

Ravi U. Sheth and Harris Wang

Department of Systems Biology, Columbia University, New York, NY

57. Engineered Bacteria to Assess and Augment Human Performance: Expanding

Sensing Capabilities

Amy M. Ehrenworth Breedon1,2, Kathryn Beabout1,2, Christina Davis1,2, Mao Taketani3,

Christopher A. Voigt3, Jorge L. Chávez2, Nancy Kelley-Loughnane4, and Michael

Goodson2

(1)Integrative Health and Performance Sciences, UES, Inc., Dayton, OH, (2)711th

Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force

Base, OH, (3)Synthetic Biology Center, Department of Biological Engineering,

Massachusetts Institute of Technology, Cambridge, MA, (4)Materials and Manufacturing

Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, OH

58. Just Add DNA: Occam Cloning Facilitates One-Step and Scarless In Vivo Assembly

of Expression Vectors

Maja Rennig1,2, Carolyn Bayer1, Anja Ehrmann1, and Morten H.H. Nørholm1,2

(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark

59. Autonomous Platform for Protein Design

Jacob Rapp1, Bennett Bremer2, and Philip A. Romero3

(1)Biochemistry, University of Wisconsin-Madison, Madison, WI, (2)Biochemistry,

University of Wisconsin - Madison, Madison, WI, (3)Department of Biochemistry,

University of Wisconsin-Madison, Madison, WI

60. Deep Learning for RNA Synthetic Biology

Nicolaas M. Angenent-Mari1,2, Luis R. Soenksen3,4, Alexander S. Garruss5, Katherine

M. Collins6, Diogo M. Camacho7, George M Church8, Timothy K Lu9, and James J

Collins10

(1)Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA,

(2)Living Cellular Devices, 2Wyss Institute for Biologically Inspired Engineering,

Boston, MA, (3)Mechanical Engineering, Massachusetts Institute of Technology,

Cambridge, MA, (4)Living Celular Devices, Wyss Institute at Harvard, Boston, MA,

(5)Living Machines, Wyss Institute for Biologically Inspired Engineering, Boston, MA,

(6)Electrical Engineering and Computer Science, Massachusetts Institute of Technology,

Cambridge, MA, (7)System Discovery, Wyss Institute for Biologically Inspired

Engineering, Boston, MA, (8)Genetics, Harvard Medical School, Boston, MA,

(9)Biological Engineering and Electrical Engineering and Computer Science, MIT,

Boston, MA, (10)Wyss Institute for Biologically Inspired Engineering, Harvard

University, Boston, MA

61. Controlled Assembly of RNA-Protein Nanostructures in Escherichia coli

Michael T. Nguyen1, Georgios Pothoulakis1, Cody Geary2, and Ebbe S. Andersen1

(1)Interdisciplinary Nanoscience Center and Department of Molecular Biology and

Genetics, Aarhus University, Aarhus, Denmark, (2)Bioengineering department,

California Institute of Technology, Pasadena, CA

62. Rapid Construction of Highly Multiplexed Gene Pools

Nathan Raynard, Elian Lee, Siyuan Chen, and Rebecca Nugent

Twist Bioscience, San Francisco, CA

63. Leveraging Twist Matrixed Oligo Pools for Multiplexed Gene Editing

Elian Lee, Rebecca Nugent, and Siyuan Chen

Twist Bioscience, San Francisco, CA

64. Creating Diverse Combinatorial Pathways with High-Throughput DNA Assembly

Nathan Raynard, Siyuan Chen, and Rebecca L Nugent

Twist Bioscience, San Francisco, CA

65. Improving Desiccation Tolerance in Synechococcus Sp. PCC 7002 Towards

Regeneration of Biomaterials

Juliana Artier1, Jishen Qiu2, Sarah L. Williams2, Mija H. Hubler2, Wil V. Srubar III2,

Sherri M. Cook2, and Jeffrey C. Cameron1,3,4

(1)Renewable & Sustainable Energy Institute (RASEI), University of Colorado Boulder,

Boulder, CO, (2)Department of Civil, Environmental, and Architectural Engineering,

University of Colorado Boulder, (3)Department of Chemistry and Biochemistry,

University of Colorado Boulder, (4)National Renewable Energy Laboratory (NREL),

Golden, CO

66. Engineering Multi-Input/Single-Output Systems in Yeast for Neural Network

Computation

Alberto Carignano1, Cannon Mallory1, and Eric Klavins2

(1)Electrical Engineering, University of Washington, Seattle, WA, (2)Electrical and

Computer Engineering, University of Washington, Seattle, WA

67. Development of Synthetic Biology Kits for STEM Education

Andrew Walters1, Ron Sims1, Ryan Jackson2, and Charles Miller1

(1)Biological Engineering, Utah State University, Logan, UT, (2)Chemistry and

Biochemistry, Utah State University, Logan, UT

68. A Tunable Integrase Differentiation Circuit Improves Output of Burdensome

Functions

Rory Williams1 and Richard M. Murray2

(1)Bioengineering, Caltech, Pasadena, CA, (2)Division of Biology and Biological

Engineering, California Institute of Technology, Pasadena, CA

69. Living Foundations: Building Hierarchical Materials with Synthetic Biology

Marimikel Charrier1, Dong Li1, Victor Mann1, Lisa Yun2, Sneha Jani1, Behzad Rad1,

Bruce Cohen3, Paul Ashby1, Kathleen Ryan4, and Caroline Ajo-Franklin1

(1)The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA,

(2)Michigan State University, East Lansing, MI, (3)Lawrence Berkeley National

Laboratory, Berkeley, CA, (4)Department of Plant & Microbial Biology, University of

California, Berkeley, CA

70. High Throughput Approaches to Dissecting Gene Regulation of Biosynthetic Gene

Clusters

Jimin Park Department of Systems Biology, Columbia University, New York, NY

71. High-Throughput Anaerobic Platform to Turbocharge Strain Engineering of Gas

Fermenting Microorganisms

Fungmin (Eric) Liew1, Michael Köpke2, and Sean D. Simpson2

(1)Synthetic Biology, LanzaTech Inc, Skokie, IL, (2)LanzaTech, Inc., Skokie, IL

72. Synthetic Biology Approaches for Improving Production of Semi-Synthetic

Artemisinin

Stephanie H. Kung, Hanxiao Jiang, Abhishek Murarka, Timothy Dobbs, Sean Lund, Will

Corcoran, and Christopher J. Paddon

Amyris, Emeryville, CA

73. Broad Loading Substrate Specificity, Diverse Extenders, and Defined ß-

Oxidation for a Platform Polyketide Synthase

Amin Zargar1, Ravi Lal2, Jessica Wang2, Miranda Werts2, Andrew Wong2, Luis

Valencia2, Constance Bailey2, Veronica T. Benites3, Edward E. K. Baidoo3, Leonard

Katz4, and Jay Keasling5

(1)Chemical Engineering, UC Berkeley, Berkeley, CA, (2)The Joint BioEnergy Institute,

Emeryville, CA, (3)Biological Systems and Engineering Division, Lawrence Berkeley

National Laboratory, Berkeley, CA, (4)QB3, University of California, Berkeley, Berkeley,

CA, (5)Biological Systems and Engineering Division, Lawrence Berkeley National

Laboratory, Emeryville, CA

74. Continuous Directed Evolution of Tryptophan Synthase Toward Production of

Unnatural Aromatic Amino Acids

Gordon Rix University of California Irvine, Irvine, CA

75. Cell-Free System in Aqueous Two-Phase Enables Multiplexing of Small Molecule

and Nucleic Acids

Yan Zhang1, Taisuke Kojima2, Shuichi Takayama2, and Mark P. Styczynski1

(1)School of Chemical and Biomolecular Engineering, Georgia Institute of Technology,

Atlanta, GA, (2)Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA

76. Expanding the Diversity of Reporters for Use in Multiplexed Cell-Free Protein

Synthesis Systems for Paper Based Sensing Platforms

Caitlin Sharpes1,2, John McManus1, Steven Blum1, James Myslinski1, Glory Mgboji3,

Vanessa Funk1, Stephanie Cole1, Peter Emanuel1, and Matthew Lux1

(1)CCDC Chemical Biological Center, Aberdeen Proving Ground, MD, (2)Excet, Inc.,

Springfield, VA, (3)Oak Ridge Institute for Science and Education, Oak Ridge, TN

77. Engineering Mammalian Cells to Record Their Own History

Courtney Carlson Biomedical Engineering, UC Irvine, Irvine, CA

78. Engineering of Lactobacillus Reuteri As a Biotherapeutic Delivery System

Annie Goodwin1, Laura Ortiz2, Laura Schafer1, Melinda Engevick1, Heather Danhof1,

Jeff Tabor3, and Robert Britton4

(1)Baylor College of Medicine, Houston, TX, (2)Rice University, houston, TX,

(3)Department of Bioengineering, Rice University, Houston, TX, (4)MVM, Baylor

College of Medicine, Houston, TX

79. Anti-CRISPR-Mediated Control of Gene Editing and Synthetic Circuits in

Eukaryotic Cells

Muneaki Nakamura1 and Stanley L. Qi2

(1)Stanford University, Stanford, CA, (2)Bioengineering, Chemical and Systems Biology,

Stanford, Stanford, CA

80. Development of a Commercially Available Targeted Integration CHOZN® gs -/-

Cell Lin

James Ravellette1, Mike Johns1, Jason Tuter1, Joaquina Mascarenhas1, Trissa

Borgschulte2, and Scott Bahr1

(1)Cell Sciences and Development, MilliporeSigma, St. Louis, MO, (2)Process Solutions,

MilliporeSigma, St. Louis, MO

81. Proteomics-Driven Genome Remodeling to Boost Cellular Capacity for Engineered

Functions

Adam Chazin-Gray Pacific Northwest National Laboratory, Richland, WA

82. Optimal Protein Reclamation with Maxmass

Neeraj Kumar1, Jeremy D. Zucker1, Jeremy Teuton1, Robert Egbert2, and Joseph

Cottam3

(1)Computational Biology, Pacific Northwest National Laboratory, Richland, WA,

(2)Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA,

(3)Visual Analytics, Pacific Northwest National Laboratory, Richland, WA

83. Development of a CRISPR-Mediated Methodology to Re-Write Microalgal

Genomes

Alexandra Mystikou1, Weiqi Fu2, David Nelson2, and Kourosh Salehi-Ashtiani1

(1)Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi,

Abu Dhabi, United Arab Emirates, (2)Division of Science and Math, New York

University Abu Dhabi, Abu Dhabi, United Arab Emirates

84. A Cell-Free Expression System for the Site-Specific Incorporation of Natural Amino

Acid Probes

Annika Urbanek1, Anna Morató1, Matija Popovic1, Carlos A. Elena-Real1, Frédéric

Allemand1, Aurélie Fournet1, Ana M. Gil2, Anabel I. Jiménez2, Carlos Cativiela2,

Stéphane Delbecq3, Nathalie Sibille1, and Pau Bernadó1

(1)Centre de Biochimie Structurale, Montpellier, France, (2)Department of Organic

Chemistry/ISQCH, University of Zaragoza-CSIC, Zaragoza, Spain, (3)Faculté de

Pharmacie, Université de Montpellier, Montpellier, France

85. Rewiring the Bacterial Genome By Retromutagenesis for Tolerance Development in

Microbial Cell Factories

Sophia A. H. Heyde and Morten H.H. Nørholm

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark,

Kgs. Lyngby, Denmark

86. CRISPR-cas9 Platform Development for a Wild-Type White-Rot Fungus Cerrena

Unicolor BBP6

Hao Zhang1 and Anli Geng2

(1)Ngee Ann Polytechnic, Singapore, Singapore, (2)School of Life Sciences and

Technology, Ngee Ann Polytechnic, Singapore, Singapore

87. Development of an Insoluble Protein Biosensor and Application in Yeast Cell

Factories

David Romero-Suarez1, Yixin Rong2, Tadas Jakociunas3, Michael K. Jensen4, and Jay

Keasling5,6,7,8,9

(1)DTU Biosustain, Technical University of Denmark, Kgs. Lyngby, Denmark, (2)Novo

Nordisk Foundation Center for Biosustainability, Kongens Lyngby, Denmark,

(3)Technical University of Denmark, The Novo Nordisk Foundation Center for

Biosustainability, Kgs. Lyngby, Denmark, (4)Novo Nordisk Foundation Center for

Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark,

(5)Biological Systems and Engineering Division, Lawrence Berkeley National

Laboratory, Emeryville, CA, (6)Department of Chemical and Biomolecular Engineering

& Department of Bioengineering, University of California, Berkeley, Berkeley, CA,

(7)Center for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes

for Advanced Technologies, Shenzhen, China, (8)Joint BioEnergy Institute, Department

of Energy, Emeryville, CA, (9)The Novo Nordisk Foundation Center for

Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark

88. Discovering Druggable Gene Combinations for Parkinson's Disease by CombiGEM-

CRISPR

Ka Ching Chan1 and Alan Siu Lun Wong1,2

(1)School of Biomedical Sciences, The University of Hong Kong, Hong Kong, Hong

Kong, (2)Department of Electrical and Electronic Engineering, The University of Hong

Kong, Hong Kong, Hong Kong

89. Synthetic Enzymology: Directed Computational Evolution of Quorum-Quenching

Lactonases

Maybelle Kho Go1,2, Bo Xue1,2, Lina Zhao3, Robert C. Robinson4, Hao Fan3, and Wen

Shan Yew1,2

(1)Biochemistry, National University of Singapore, Singapore, Singapore, (2)NUS

Synthetic Biology for Clinical and Technological Innovation, Singapore, Singapore,

(3)Bioinformatics Institute, A*STAR, Singapore, Singapore, (4)Okayama University,

Okayama, Japan

90. Engineering the Human Compass Family of Histone H3K4 Methylases in Yeast

Max Haase and Jef Boeke

Institute for Systems Genetics, NYU Langone Health, New York, NY

91. Design and Rapid Emulation of a Synthetic Microbial Operational Amplifier on

Cytomorphic Silicon Chips

Jonathan Teo1, JI Zeng2, Sung Sik Woo3, and Rahul Sarpeshkar2

(1)Massachusetts Institute of Technology, Cambridge, MA, (2)Dartmouth College,

Hanover, NH, (3)Formlabs, Somerville, MA

92. Natural and Directed Evolution of Substrate Specificity in Family 5 Glycoside

Hydrolases

Evan Glasgow1,2, Kirk Vander Meulen1,2, Taichi E. Takasuka1,2,3, Christopher

Bianchetti1,2,4, Lai Bergeman1,2, Samuel Deutsch5, and Brian G. Fox1,2

(1)Great Lakes Bioenergy Research Center, Madison, WI, (2)Biochemistry, University of

Wisconsin - Madison, Madison, WI, (3)Hokkaido University, Sapporo, Japan,

(4)Chemistry, University of Wisconsin - Oshkosh, Oshkosh, WI, (5)DOE Joint Genome

Institute, Walnut Creek, CA

93. Cell Free Transcription-Translation for Natural Product Research

Patrick D. Capel, Christophe Corre, and Emzo de los Santos

University of Warwick, Coventry, United Kingdom

94. Whole-Cell Living Bioreporters for Butanol Production: Directed Evolution of a

Transcriptional Regulator Towards Short-Chain Alcohols

Maximilian O. Bahls and Sven Panke

Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland

95. Beacon Riboregulators: Exploiting Loop-Mediated RNA-RNA Interactions for

Molecular Computing and Diagnostics

Duo Ma The Biodesign Institute and the School of Molecular Sciences, Arizona State University,

Tempe, AZ

96. Simultaneous Regulation of Many Genes Using Crispri with Highly Non-Repetitive

Extra Long Sgrna Arrays (ELSAs)

Alexander Reis1, Sean Halper1, Grace Vezeau2, Daniel Cetnar1, Ayaan Hossain3, Phillip

Clauer4, and Howard M. Salis5

(1)Chemical Engineering, Penn State University, University Park, PA, (2)Pennsylvania

State University, University Park, PA, (3)Bioinformatics and Genomics, Penn State

University, University Park, PA, (4)Biochemistry and Molecular Biology, Pennsylvania

State University, University Park, PA, (5)Department of Chemical Engineering /

Biological Engineering, Pennsylvania State University, University Park, PA

97. Synchronising a Population of Yeast Cells By Microfluidics-Based Feedback

Control

Giansimone Perrino1, Davide Fiore2, Sara Napolitano3,4, Mario di Bernardo5,6, and

Diego di Bernardo1,4

(1)Telethon Institute of Genetics and Medicine, Pozzuoli, Italy, (2)Electric Engineering

and Information Technology, University of Naples Federico II, Naples, Italy, (3)Telethon

Institute of Genetics and Medicine, Naples, Italy, (4)Chemical, Materials and Industrial

Production Engineering, University of Naples Federico II, Naples, Italy, (5)Department

of Electrical Engineering and Information Technology, University of Naples Federico II,

Naples, Italy, (6)University of Bristol, Bristol, United Kingdom

98. Dynamic Control of Pathway Expression with Riboregulated Switchable Feedback

Promoters

Cameron J. Glasscock1, Tyler Lazar2, Bradley W. Biggs2, Jack Arnold2, Min-Kyoung

Kang2, Danielle Tullman-Ercek2, Keith E.J. Tyo2, and Julius B. Lucks2

(1)Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell

University, Ithaca, NY, (2)Chemical and Biological Engineering, Northwestern

University, Evanston, IL

99. Diagnosing Limitations to High Titer Ethanol Production in Clostridium

Thermocellum Using a Cell-Free System

Daniel Olson1, Jingxuan Cui1, Daniel Amador-Noguez2, and Lee R. Lynd3

(1)Thayer School of Engineering, Dartmouth College, Hanover, NH, (2)Bacteriology,

University of Wisconsin - Madison, Madison, WI, (3)Thayer School of Engineering,

Department of Biological Sciences, Dartmouth College, Hanover, NH

100. Automated DNA Construction: From j5 Protocol Design to Laboratory

Robotics

Nurgul Kaplan1,2,3, Tadeusz Ogorzalek1,2,3, Joel M. Guenther4, Garima Goyal5, Jennifer

Chiniquy1,2, Jonathan Diab1,2,6, Henrique C. De Paoli1, and Nathan J Hillson1,2,3

(1)DOE Agile BioFoundry, EMERYVILLE, CA, (2)Biological Systems and Engineering

Division, Lawrence Berkeley National Laboratory, Berkeley, CA, (3)Technology

Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA, (4)Sandia National

Laboratory, Livermore, CA, (5)Fuels Synthesis Division, Joint BioEnergy Institute,

Emeryville, CA, (6)Technology Division, DOE Joint BioEnergy Institute, EMERYVILLE,

CA

101. Validation and Feedback Control of a Reference-Comparator System within

Escherichia coli

Barbara Shannon, Fabio Annunziata, Gianfranco Fiore, Antoni Matyjaszkiewicz, Claire

Grierson, Lucia Marucci, Nigel Savery, and Mario di Bernardo

University of Bristol, Bristol, United Kingdom

102. High-Throughput Genome Engineering for Industrial Strain Optimization

Michael Martyn III Platform R&D, Zymergen, Emeryville, CA

103. Engineering Prototrophy in Mammalian Cells

Julie Trolle1, Ross McBee2, Liyuan Liu2, Andrew Kaufman2, Xinyi Guo1, Sudarshan

Pinglay1, Henri Berger1, Sergei German1, Harris Wang2, and Jef Boeke1

(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)Department of

Systems Biology, Columbia University, New York, NY

104. Mapping Ribozyme Fitness Landscapes to Investigate Evolutionary

Optimization and Divergence

Evan Janzen Biomolecular Science and Engineering, University of California, Santa Barbara, Santa

Barbara, CA

105. Sugar Synthesis from CO2 in Escherichia coli

Shmuel Gleizer1, Niv Antonovsky1, Elad Herz1, Elad Noor2, Yehudit Zohar1, Arren Bar-

Even3, and Ron Milo1

(1)Weizmann Institute of Science, Rehovot, Israel, (2)Institute of Molecular Systems

Biology, ETH Zurich, Zurich, Switzerland, (3)Systems and Synthetic Metabolism, Max

Planck Institute for Molecular Plant Physiology, Potsdam, Germany

106. Engineered History-Dependent Behavior in a Multicellular System

Ana Zuñiga1, Sarah Guiziou1, Ben Meriem Zacchary2, Pauline Mayonove1, Violaine

Moreau1, Luca Ciandrini3, Pascal Hersen2, and Jerome Bonnet1

(1)Centre de Biochimie Structurale, Montpellier, France, (2)Laboratoire Matière et

Systèmes Complexes, Paris, France, (3)Laboratoire Charles Coulomb, Montpellier,

France

107. Tunning Cross Membrane Signal Transmission for Synthetic Receptor

Engineering

HungJu Chang1, Jerome Gracy1, Pauline Mayonove1, Martin Cohen-Gonsaud1,

Guillaume Cambray2, and Jerome Bonnet1

(1)Centre de Biochimie Structurale, Montpellier, France, (2)INRA, UMR DGIMI,

Montpellier, France

108. Human Milk Oligosaccharides - Enabling Novel Health Benefit Applications

through the Glycoactives Platform

Joeri Beauprez1 and Wesley Carpentier2

(1)Inbiose N.V., Zwijnaarde, Belgium, (2)Inbiose NV, Zwijnaarde, Belgium

109. Engineering Asymmetrical Cell Division into Escherichia coli

Sara Molinari Department of Biosciences, Rice University, Houston, TX

110. Plug-and-Play Metabolic Transducers Expand the Chemical Detection Space

of Cell-Free Biosensors

Peter Voyvodic1, Amir Pandi2, Ismael Conejero1,3,4, Emmanuel Valjent5, Philip

Courtet3,6, Eric Renard5,7, Jean-Loup Faulon2,8, and Jerome Bonnet1

(1)Centre de Biochimie Structurale, Montpellier, France, (2)MICALIS Institute, INRA,

Jouy-en-Josas, France, (3)INSERM U1061, Neuropsychiatry, Epidemiological and

Clinical Research, Montpellier, France, (4)Department of Psychiatry, Caremeau

Hospital, University Hospital of Nîmes, Nîmes, France, (5)Institute of Functional

Genomics, UMR CNRS 5203/INSERM U1191, Montpellier, France, (6)Department of

Emergency Psychiatry and Post Acute Care, CHU Montpellier, Lapeyronie Hospital,

Montpellier, France, (7)Department of Endocrinology, Diabetes, Nutrition and CIC,

Montpellier University Hospital, Montpellier, France, (8)SYNBIOCHEM Center,

University of Manchester, Manchester, United Kingdom

111. Refurbishing the Fluorescence Complementation Assay Landscape - Design

and Construction of Mammalian Expression Vector Sets for Improved Signal-to-

Noise Ratios in Protein-Protein-Interaction Analyses

Raluca Fleischer1,2 and Gary R. Lewin3

(1)Max-Delbrück-Zentrum, Berlin, Germany, (2)Humboldt-Universität zu Berlin, Berlin,

Germany, (3)Max-Delbrück-Zentrum

112. High Throughput Strain Engineering at Ginkgo Bioworks

Alicja Gomes Ginkgo Bioworks, Boston, MA

113. A Proteome Allocation Model to Improve Photosynthetic Productivity and

Efficiency in a Light-Limited Chemostat

Marjan Faizi and Ralf Steuer

Institute for Theoretical Biology, Humboldt Universität zu Berlin, Berlin, Germany

114. Molecular Sequestration Strategies for Quorum Sensing Systems

Nicholas DeLateur1, Christian E. Cuba Samaniego2, and Ron Weiss3

(1)Department of Biological Engineering, M.I.T., Cambridge, MA, (2)Department of

Biological Engineering, MIT, Cambridge, MA, (3)Biological Engineering, M.I.T.,

Cambridge, MA

115. Exploring Genetic Variation Using Retron Library Recombineering (RLR)

Max G. Schubert1, Daniel B. Goodman2, Seth Shipman3, and George M Church4

(1)Harvard Medical School, Boston, MA, (2)Wyss Institute for Biologically Inspired

Engineering, Boston, MA, (3)Gladstone Institutes, San Francisco, CA, (4)Genetics,

Harvard Medical School, Boston, MA

116. Identification and Experimental Manipulation of Surface Colonization

Pathways in a Model Marine Diatom

Weiqi Fu1, Amphun Chaiboonchoe1, Mehar Sultana2, Kristos Baffour1, Dina Al-Khairy1,

Sarah Daakour1, Alexandra Mystikou2, Amnah Alzahmi1, Ashish Jaiswal1, David Nelson1,

Sigurdur Brynjolfsson3, and Kourosh Salehi-Ashtiani2

(1)Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, United

Arab Emirates, (2)Center for Genomics and Systems Biology (CGSB), New York

University Abu Dhabi, Abu Dhabi, United Arab Emirates, (3)Center of Systems Biology,

University of Iceland, Iceland

117. Personalized Off-Target Analysis of CRISPR-Cas9 gRNA Designs Using in-

Silico and Experimental Approaches

Yidan Pan, Ciaran Lee, and Gang Bao

Department of Bioengineering, Rice University, Houston, TX

118. Increasing Functionality of GFP in Human Cells

Cansu Kuey, Gabrielle Larocque, Nicholas Clarke, and Stephen Royle

Centre for Mechanochemical Cell Biology, Warwick Medical School, Coventry, United

Kingdom

119. Multiplexing Cell-Cell Communication

John T. Sexton and Jeffrey J. Tabor

Department of Bioengineering, Rice University, Houston, TX

120. Cell-Free Styrene Biosynthesis at High Titers

Blake J. Rasor1,2, William S. Grubbe3, Ashty S. Karim1,2, and Michael C. Jewett1,2,4

(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,

(2)Center for Synthetic Biology, Northwestern University, Evanston, IL, (3)Institute for

Molecular Engineering, University of Chicago, Chicago, IL, (4)Chemistry of Life

Processes Institute, Northwestern University, Evanston, IL

121. Engineered Probiotic for the Inhibition of Salmonella Via Tetrathionate-

Induced Production of Microcin H47

Benedikt Mortzfeld, Jacob Palmer, and Vanni Bucci

Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA

122. "Big DNA" Construction and Characterization of Alpha-Globin Locus

Variants

Brendan R. Camellato1, Leslie A. Mitchell1, Helena Francis2, Mira T. Kassouf2, Matthew

T. Maurano1, Douglas R. Higgs2, and Jef Boeke1

(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)MRC

Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United

Kingdom

123. Engineering Cupriavidus Necator for Autotrophic 1,3-Butanediol Production

Joshua Gascoyne1, Rajesh Bommareddy2, Stephan Heeb1, and Naglis Malys1

(1)Synthetic Biology Research Centre, Centre for Biomolecular Sciences, University of

Nottingham, Nottingham, United Kingdom, (2)Synthetic Biology Research Centre, Centre

for Biomolecular Sciences, University of Nottingham, United Kingdom

124. Molecular Crowders Regulate Gene Expression in an E. coli-Based Cell-Free

System

Grace Vezeau and Howard Salis

Pennsylvania State University, University Park, PA

125. Massively-Parallel Dissection of Human Transcription Factor Binding Site

Architecture

Jessica Davis1, Kimberly Insigne2, Eric M. Jones3, Quinn Hastings4, and Sriram Kosuri5

(1)Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA,

(2)Bioinformatics IDP, UCLA, Los Angeles, CA, (3)Octant, Los Angeles, CA, (4)UCLA,

Los Angeles, CA, (5)Department of Chemistry and Biochemistry, University of

California, Los Angeles, Los Angeles, CA

126. Software-Enable Design and Automated Construction of CRISPR Gene

Circuits in S. Cerevisiae

Justin Vrana1 and Eric Klavins2

(1)Bioengineering, UW-Seattle, Seattle, WA, (2)Electrical and Computer Engineering,

University of Washington, Seattle, WA

127. An Engineered Light-Switchable Two-Component System for Dynamic

Interrogation of Cell-Fate Decision Networks in Bacillus Subtilis

Sebastian M. Castillo-Hair1, Elliot A. Baerman2,3, Masaya Fujita4, Oleg Igoshin1, and

Jeffrey J. Tabor1

(1)Department of Bioengineering, Rice University, Houston, TX, (2)Rice University,

Houston, TX, (3)Baylor College of Medicine, Houston, TX, (4)University of Houston,

Houston, TX

128. Optimal Experimental Design for a Bistable Gene Regulatory Network

Nathan Braniff, Addison Richards, and Brian Ingalls

University of Waterloo, Waterloo, ON, Canada

129. Interfacing a Transcriptional Biosensing with Toehold-Mediated Strand

Displacement for Programmable Molecular Diagnostics

Jaeyoung K. Jung1,2, Khalid K. Alam1,2, and Julius B. Lucks1,2,3,4

(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,

(2)Center for Synthetic Biology, Northwestern University, Evanston, IL,

(3)Interdisciplinary Biological Sciences Program, Northwestern University, Evanston,

IL, (4)Chemistry of Life Processes Institute, Northwestern University, Evanston, IL

130. Acoustic Detonation of Cellular Therapies Via Biomolecular Cavitation

Avinoam Bar-Zion1, Atousa Nourmahnad1, David Mittelstein2, Sangjin Yoo1, Dina

Malounda1, Mohamad Abedi3, Audrey Lee-Gosselin1, David Maresca1, and Mikhail G.

Shapiro1

(1)Division of Chemistry and Chemical Engineering, California Institute of Technology,

Pasadena, CA, (2)Division of Engineering and Applied Science, California Institute of

Technology, Pasadena, CA, (3)Division of Biology and Biological Engineering,

California Institute of Technology, Pasadena, CA

131. Programmable Polyketide Synthase (PKS) Platform and an Artificial

Biosynthetic Pathway for the Natural Food Pigment Carmine

Rasmus John Norman Frandsen1, Kresten Jon Kromphardt Olsen2, Paiman Khorsand-

Jamal3, Kenneth T. Kongstad4, Majse Nafisi5, Rubini Kannangara6, Birger Lindberg

Møller7, Thomas Ostenfeld Larsen2, and Uffe H. Mortensen1

(1)DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark,

(2)DTU Bioengineering, Technical University of Denmark, Kgs. Lyngby, Denmark,

(3)Technical University of Denmark, Kgs. Lyngby, Denmark, (4)Department of Drug

Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark, (5)Chr.

Hansen Natural Colors A/S, Hoersholm, Denmark, Horsholm, Denmark, (6)Chr. Hansen

Natural Colors A/S, Hoersholm, Denmark, (7)Copenhagen Plant Science Centre,

Department of Plant and Environmental Sciences, University of Copenhagen,

Copenhagen, Denmark

132. Single-Cell Characterization of Gene Regulatory Functions in the S.

Cerevisiae Inorganic Phosphate Starvation Response Network

Evan J. Olson1, Hon Ming Yip1, Michael A. Crone2, Shiyu Cheng1, and Sebastian J.

Maerkl1

(1)Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de

Lausanne, Lausanne, Switzerland, (2)Department of Medicine, Imperial College London,

London, United Kingdom

133. High-Throughput Microfluidic Platform for Gene Regulatory Network

Characterization at the Single Cell Level

Hon Ming Yip1, Evan J. Olson1, Michael A. Crone2, Shiyu Cheng1, and Sebastian J.

Maerkl1

(1)Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de

Lausanne, Lausanne, Switzerland, (2)Department of Medicine, Imperial College London,

London, United Kingdom

134. Metabolic Engineering of Bacillus Subtilis for Biopolymer Production from

Biodiesel Waste Material

Lorenzo Pasotti1, Ilaria Massaiu1, Erlinda Rama2, Matteo Cavaletti2, Nikolaus

Sonnenschein3, Markus J. Herrgård4, Paolo Magni1, and Cinzia Calvio5

(1)Dep. of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia,

Italy, (2)Biology and Biotechnology, University of Pavia, Pavia, Italy, (3)The Novo

Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs.

Lyngby, Denmark, (4)Novo Nordisk Foundation Center for Biosustainability, Technical

University of Denmark, Kongens Lyngby, Denmark, (5)Department of Biology and

Biotechnology, University of Pavia, Pavia, Italy

135. Screening for Peptide Inhibitors of S. typhimurium PhoPQ in E. coli

Kathryn Brink1, Andrew Mu2, Bryan Davies3, and Jeff Tabor4

(1)PhD Program in Systems, Synthetic, and Physical Biology, Rice University, Houston,

TX, (2)Department of Biosciences, Rice University, Houston, TX, (3)Department of

Molecular Biosciences, UT Austin, Austin, TX, (4)Department of Bioengineering, Rice

University, Houston, TX

136. Beyond Hill Equations: Mechanistic Modeling of Inducible Systems to

Expand the Predictability of Synthetic Circuits

Davide De Marchi1, Lorenzo Pasotti2, Massimo Bellato2, and Paolo Magni2

(1)Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy,

(2)Dep. of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia,

Italy

137. How Resource Competition Shapes the Stability Profile of Toggle Switches at

the Cellular and at the Population Levels

Andras Gyorgy Electrical Engineering, New York University Abu Dhabi, Abu Dhabi, United Arab

Emirates

138. Carbon Monoxide Tolerance and Utilisation in Cupriavidus Necator

Charlie Wickham-Smith1, Naglis Malys2, and Klaus Winzer3

(1)Synthetic Biology Research Centre, University of Nottingham, Nottingham, United

Kingdom, (2)Synthetic Biology Research Centre, Centre for Biomolecular Sciences,

University of Nottingham, Nottingham, United Kingdom, (3)School of Life Sciences,

University of Nottingham, Nottingham, United Kingdom

139. Prototyping Protein-Protein Interaction-Based Logic Using Cell-Free Protein

Synthesis

Andrew Hunt1, Zibo Chen2, David Baker2, and Michael C. Jewett1

(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,

(2)Department of Biochemistry, University of Washington, Seattle, WA

140. CRISPR-Cas Based Capture of Horizontal Gene Transfer in Complex

Communities

Christian Munck1, Ravi U. Sheth1, Danie E. Freedberg2, and Harris Wang1

(1)Department of Systems Biology, Columbia University, New York, NY, (2)Division of

Digestive and Liver Diseases, Columbia University Medical Center, New York, NY

141. Combinatorial Design of Protein Switches for Controlling Electron Transfer

in Cells

Bingyan Wu1, Joshua T. Atkinson2, George N. Bennett3, and Jonathan J. Silberg4

(1)Biosciences, Rice University, Houston, TX, (2)Systems, Synthetic, and Physical

Biology Graduate Program, Rice University, Houston, TX, (3)Department of Biosciences,

Rice University, Houston, TX, (4)Biosciences and Bioengineering, Rice University,

Houston, TX

142. A Synthetic Genomics Approach to Dissecting an Age-Related Macular

Degeneration-Associated Haplotype

Jon M Laurent1, Xin Fu2, Sergei German1, Ran Brosh1, Kang Zhang2, Matthew T.

Maurano1, and Jef Boeke1

(1)Institute for Systems Genetics, NYU Langone Health, New York, NY, (2)Shiley Eye

Institute, University of California San Diego School of Medicine, San Diego, CA

143. Engineering Multicellular 3D Shapes

Jesse Tordoff1, Matej Krajnc2, Nicholas Walczak3, Jacob Beal3, Stanislav Y.

Shvartsman2, and Ron Weiss4

(1)CSB, MIT, Cambridge, MA, (2)Chemical and Biological Engineering, Princeton

University, Princeton, NJ, (3)Raytheon BBN Technologies, Cambridge, MA,

(4)Biological Engineering, M.I.T., Cambridge, MA

144. Development of a Gene Signal Amplifier Platform Technology for

Monitoring the Unfolded Protein Response

Carlos A. Origel1, Bhagyashree K. Bachhav2, and Laura Segatori3

(1)Bioscience Department, Rice University, Houston, TX, (2)Department of Chemical

and Biomolecular Engineering, Rice University, Houston, TX, (3)Bioengineering, Rice

University, Houston, TX

145. Engineering a Gas-Producing Biosensor to Monitor Nitrate Bioavailiability

in Terrestrial Soils and Marine Sediments

Emily M. Fulk1, Ilenne Del Valle1, Xiaodong Gao2, Caroline A. Masiello3, and Jonathan

J. Silberg4

(1)Systems, Synthetic, and Physical Biology, Rice University, Houston, TX, (2)Earth,

Environmental and Planetary Sciences, Rice University, Houston, TX, (3)Earth Sciences,

Rice University, Houston, TX, (4)Biosciences and Bioengineering, Rice University,

Houston, TX

146. Independent Control of Mean and Noise By Convolution of Gene Expression

Distributions

Karl Gerhardt1, Evan J. Olson2, and Jeff Tabor1

(1)Department of Bioengineering, Rice University, Houston, TX, (2)Institute of

Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne,

Lausanne, Switzerland

147. Application of Terminator, Protein Degradation, and Protein Solubility

Libraries for Strain Improvement

Matthew Davis, Chris Wisnewski, and Patrick Westfall

Zymergen, Emeryville, CA

148. A High-Throughput Assay to Investigate the Antibiotic Activity of the Lasso

Peptide Klebsidin

Ethan Hills, Benjamin Brandsen, and Stanley Fields

Department of Genome Sciences, University of Washington, Seattle, WA

149. Synthetic Biologic Tools for Microbial Ecology and Biogeochemistry

David L. Shis1, Ilenne Del Valle2, Emily M. Fulk3, Johnathan J. Silberg4, and Caroline A.

Masiello5

(1)Department of Biosciences, Rice University, Houston, TX, (2)Systems, Synthetic, and

Physical Biology, Rice University, Houston, TX, (3)Chemical and Biological

Engineering, Northwestern University, Evanston, IL, (4)Biochemistry & Cell Biology,

Rice University, Houston, TX, (5)Earth Sciences, Rice University, Houston, TX

150. Exploring the Sequence Space of a Riboswitch Expression Platform Yields

Tunable Switching Efficacy

Gregory W. Campbell Biomolecular Science and Engineering, University of California, Santa Barbara, Santa

Barbara, CA

151. Mining Pams from Diverse Bacterial Genomes

Florencia Velez-Cortes and Harris Wang

Department of Systems Biology, Columbia University, New York, NY

152. Evolutionary Design of Synthetic Oligomers and Protein-Based Materials

Anna J Simon Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX

153. Using Microbial Memory to Record Horizontal Gene Transfer Events in

Situ.

Prashant Kalvapalle1, Emily M. Fulk2, Johnathan J. Silberg3, and Lauren Stadler4

(1)Systems, Synthetic and Physical Biology, Rice University, HOUSTON, TX, (2)Systems,

Synthetic, and Physical Biology, Rice University, Houston, TX, (3)Biochemistry & Cell

Biology, Rice University, Houston, TX, (4)Civil and Environmental Engineering, Rice

University, Houston, TX

154. Developments of Genetic Tools for Methane-Oxidizing Bacteria

Bashir Rumah1, Christopher Stead1, Nigel Minton2, and Ying Zhang1

(1)BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, The

University of Nottingham, Nottingham, United Kingdom, (2)BBSRC/EPSRC Synthetic

Biology Research Centre, University of Nottingham, University of Nottingham, United

Kingdom

155. Recombinase Efficiency in Human Cell Lines

George Chao1, Clair Travis1, and George M Church2

(1)Harvard Medical School, Boston, MA, (2)Genetics, Harvard Medical School, Boston,

MA

156. Metabolic Engineering of Yarrowia Lipolytica for De Novo Synthesis of 2-

Phenylehtanol from Sustainable Low-Cost Feedstocks

Yang Gu1, Long Liu2, and Peng Xu3

(1)University of Maryland, Baltimore county, Baltimore, MD, (2)Jiangnan university,

Wuxi, China, (3)Chemical, Biochemical and Environmental Engineering, University of

Maryland, Baltimore County, Baltimore, MD

157. Metabolic engineering of Yarrowia lipolytica for de novo synthesis of 2-

phenylehtanol from sustainable low-cost feedstocks Yang Gu1,2, Long Liu2 and Peng Xu1

1 Chemical, Biochemical and Environmental Engineering, UMBC, Baltimore, USA 2School of Biotechnology, Jiangnan University, Wuxi, China

158. Control of Protein Synthesis By Using Promoter Variants in Cell-Free

System

Naoko Senda1, Ruolan Zhang2, and Hirokazu Nishida3

(1)Research & Development Group, Hitachi, Ltd., Tokyo, Japan, (2)Hitachi, Ldt., Tokyo,

Japan, (3)Hitachi, Ltd., Tokyo, Japan

159. Characterization of an Integrase-Based Genetic ‘Tape Recorder’

Circuit in e coli

Andrey Shur1 and Richard M. Murray2

(1)Biology and Biological Engineering, California Institute of Technology, Pasadena,

CA, (2)Division of Biology and Biological Engineering, California Institute of

Technology, Pasadena, CA

160. Engineering Gut Inflammation Biosensors with Bacterial Stress Response

Kathleen Jia Yue Zhang1 and David R. McMillen2

(1)Cell and Systems Biology, University of Toronto, Toronto, ON, Canada, (2)Chemical

and Physical Sciences, University of Toronto, Toronto, ON, Canada

161. A Synthetic Approach for Parsing Gene Regulatory Logic in Plant

Development

Edith Pierre-Jerome and Philip Benfey

Biology, Duke University, Durham, NC

162. Synthetic Enzymology and the Fountain of Youth

Yan Ping Lim1, Maybelle Kho Go1, Matthew Wook Chang2, and Wen Shan Yew3

(1)NUS Synthetic Biology for Clinical and Technological Innovation, Singapore,

Singapore, (2)Department of Biochemistry, Yong Loo Lin School of Medicine, National

University of Singapore, Singapore, Singapore, (3)Biochemistry, National University of

Singapore, Singapore, Singapore

163. Variational Autoencoder for Generation of Antimicrobial Peptides

Scott N. Dean1 and Scott A. Walper2

(1)National Research Council, Washington, DC, (2)Center for Biomolecular Science and

Engineering, Naval Research Laboratory, Washington, DC

164. Response of Lactobacillus Plantarum WCFS1 to the Pathogen-Associated

Quorum Sensing Molecule N-3-Oxododecanoyl Homoserine Lactone

Joseph R. Spangler1, Scott N. Dean1, Dagmar Leary2, and Scott A. Walper2

(1)National Research Council, Washington, DC, (2)Center for Biomolecular Science and

Engineering, Naval Research Laboratory, Washington, DC

165. Characterization of Membrane-Bound Components in Cell-Free

Glycosylation Systems

Katherine F. Warfel1, Jasmine M. Hershewe1, Han Teng Wong1, Justin Peruzzi1, Neha

Kamat2, Danielle Tullman-Ercek1, and Michael C. Jewett1

(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,

(2)Biomedical Engineering, Northwestern University, Evanston, IL

166. Metabolic Engineering of Corynebacterium Glutamicum Strains for the

Production of Bio-Based Nylon Monomers

Si Jae Park1, Hee Taek Kim2, and Jeong Chan Joo3

(1)Division of Chemical Engineering and Materials Science, Ewha Womans University,

Seoul, Korea, Republic of (South), (2)KRICT, Daejeon, Korea, Republic of (South),

(3)Center for Bio-based Chemistry, Division of Convergence Chemistry, Korea Research

Institute of Chemical Technology, Daejeon, Korea, Republic of (South)

167. Metagenomic Platform for Rapid Natural Product Discovery

Aleksandr Milshteyn Discovery, Zymergen Inc., Emeryville, CA

168. Regulatory Aspects of Synthetic Biology in Germany

Wolfram Volkwein, Ulrich Busch, and Armin Baiker

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany

169. Development of a Complete CRISPR-Cpf1 Tool for Metabolic Engineering

of Clostridium Beijerinckii ncimb 8052

Constantinos Patinios Microbiology, Wageningen University and Research, Wageningen, Netherlands

170. Synthetic Orfeomes of Prochlorococcus MarinusMED4 and NATL1A: A

Resource for Systems-Level Interrogation of Low- and High-Light Adaptations in

Cyanobacteria

Sarah Daakour1,2, Weiqi Fu1, Ashish Jaiswal1, Amnah Alzahmi1,2, Joseph Koussa3,

Amphun Chaiboonchoe1, David Nelson1,2, and Kourosh Salehi-Ashtiani1,2

(1)Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, United

Arab Emirates, (2)Center for Genomics and Systems Biology (CGSB), New York

University Abu Dhabi, Abu Dhabi, United Arab Emirates, (3)Department of Biology,

New York University Abu Dhabi, New York, NY

171. Genetically-Encoded RNA Origami Nanostructures and Nanodevices to

Scaffold Proteins

Guido Grossi1, Cody Geary1,2, Ilenia Manuguerra1, Paul W. K. Rothemund2, and Ebbe S.

Andersen1

(1)Interdisciplinary Nanoscience Center and Department of Molecular Biology and

Genetics, Aarhus University, Aarhus, Denmark, (2)Bioengineering department,

California Institute of Technology, Pasadena, CA

172. Packaging of Diisopropyl Fluorophosphatase (DFPase) in Bacterial Outer

Membrane Vesicles Protects Its Activity at Extreme Temperature

Meghna Thakur1 and Scott A. Walper2

(1)College of Science, George Mason University, Fairfax, VA, (2)Center for

Biomolecular Science and Engineering, Naval Research Laboratory, Washington, DC

173. Programmable Protein Circuits in Living Cells

Xiaojing Gao1, Lucy Chong1, Matthew Kim1, and Michael Elowitz2

(1)Caltech, Pasadena, CA, (2)California Institute of Technology, Pasadena, CA

174. Sensing Intracellular Metabolites with RNA Nanostructures in E. coli

Ilenia Manuguerra, Mette D. E. Jepsen, Tenna T. Henriksen, Steffen M. Sparvath, and

Ebbe S. Andersen

Interdisciplinary Nanoscience Center and Department of Molecular Biology and

Genetics, Aarhus University, Aarhus, Denmark

175. Towards in Situ Engineering of Electroautotrophic Microbial Communities

Sarah Glaven Center for Bio/Molecular Science and Engineering, Naval Research Laboratory,

Washington, DC

176. Designing Vesicle Membranes to Control Spatial-Temporal Dynamics of

Biological Reactions

Justin Peruzzi1, Miranda Jacobs2, Timothy Vu3, and Neha Kamat3,4

(1)Chemical and Biological Engineering, Northwestern University, Evanston, IL,

(2)Interdisciplinary Biological Sciences, Northwestern University, Evanston, IL,

(3)Biomedical Engineering, Northwestern University, Evanston, IL, (4)Center for

Synthetic Biology, Northwestern University, Evanston, IL

177. Chi.Bio: A Low-Cost Platform for Automated Characterisation and

Manipulation of Biological Systems

Harrison Steel1, Robert Habgood2, and Antonis Papachristodoulou1

(1)Department of Engineering Science, University of Oxford, Oxford, United Kingdom,

(2)University of Oxford, Oxford, United Kingdom

178. Hydrogel Delivery of Cell-Free Protein Synthesis

Marilyn F. S. Lee1, Chia-Suei Hung2, Daniel A. Phillips3, Zachary Martinaeu4, Chelsea

Buck5, Maneesh Gupta6, and Matthew Lux7

(1)Biochemistry, US Army CCDC CBC, Aberdeen Proving Ground, MD, (2)Air Force

Research Laboratory, Dayton, OH, (3)US Naval Research Laboratory, Washington, DC,

(4)UES, Inc., Air Force Research Laboratory, Dayton, OH, (5)UES Inc., Air Force

Research Laboratory, Dayton, OH, (6)Materials and Manufacturing Directorate, Air

Force Research Laboratory, WPAFB, OH, (7)CCDC Chemical Biological Center,

Aberdeen Proving Ground, MD

179. Bioconversion of Methane to Transportation Fuel Using Environmentally

Isolated Methanotrophic Bacteria

Christopher Stead, Bashir Rumah, Nigel Minton, and Ying Zhang

BBSRC/EPSRC Synthetic Biology Research Centre, School of Life Sciences, The

University of Nottingham, Nottingham, United Kingdom

180. Expression and Purification of Highly Active Antimicrobial Peptide HBCM2

from Escherichia coli Using an Encapsulin Nanocompartment System

Tek-Hyung Lee1, Timothy S. Carpenter1, David Savage2, and Mimi C. Yung1

(1)Lawrence Livermore National Laboratory, Livermore, CA, (2)MCB and Chemistry,

UC Berkeley, Berkeley, CA

181. Temperature Sensitive Variants of the Protease, Subtilisin Carlsberg, for

Automated Processes

Vanessa C. Thompson, Tyler W. Dyer, Geoff Gudavicius, Bailey E McGuire, Max Legg,

and Francis E Nano

Biochemistry & Microbiology, University of Victoria, Victoria, BC, Canada

182. Linking Mobile Genes with Their Bacterial Hosts in Natural Gut

Communities Using OIL PCR

Peter Diebold Microbiology, Cornell University, Ithaca, NY

183. Web of Registries

Hector Plahar1, Leanne Springthorpe2, William Morrell3, Paul D. Adams4, and Nathan J

Hillson5

(1)Fuels Synthesis and Technology Divisions, Joint BioEnergy Institute, Emeryville, CA,

(2)Fuels Synthesis and Technology, Joint Bioenergy Institute, Emeryville, CA,

(3)Biomass Science & Conversion Technologies, Sandia National Laboratory,

Livermore, CA, (4)Technology Division, Joint BioEnergy Institute, Emeryville, CA,

(5)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA

184. Making Hot E. coli Hybrids: Combining Multiple Genetic Approaches for

Directed Evolution

Bailey E McGuire, Quinn E Matthews, and Francis E Nano

Biochemistry & Microbiology, University of Victoria, Victoria, BC, Canada

185. Miniaturization and Rapid Processing of Txtl Reactions Using Acoustic

Liquid Handling

Jared Bailey1, Evelyn Eggenstein2, Jean-Marie Rouillard2, and John Lesnick1

(1)Labcyte, San Jose, CA, (2)Arbor Biosciences, Ann Arbor, MI

186. Combining Scalable DNA Assembly Using the Next Universal Guided

Enzymatic (NUGE) Assembly Method with Automated Workflows

Jared Bailey1, John Lesnick1, and Rebecca L Nugent2

(1)170 Rose Orchard Way, Labcyte Inc, San Jose, CA, (2)Twist Bioscience, San

Francisco, CA

187. Autonomously Controlled and Host-Aware Recombinant Gene Expression

through Plasmid Copy Number Modulation

Nicolas Kylilis and Guy-Bart Stan

Department of Bioengineering, Imperial College London, London, United Kingdom

188. Developing a Modular Framework for Orthogonal Control of Gene

Expression in Plants

Shaunak Kar1, Yogendra Bordiya1, Nestor Rodriguez1, Elizabeth Gardner1, Junghyun

Kim1, Jimmy Gollihar2, Andrew Ellington1,3, and Sibum Sung1

(1)Molecular Biosciences, University of Texas at Austin, Austin, TX, (2)US Army

Research Laboratory- South, Austin, TX, (3)Center for Systems and Synthetic Biology,

University of Texas at Austin, Austin, TX

189. Scaling Semi-Synthetic Artemisinin Production from Plates to Industrial

Fermentation

Timothy Dobbs, Abhishek Murarka, Hanxiao Jiang, Stephanie H. Kung, Daniel Yim, and

Christopher J. Paddon

Amyris, Emeryville, CA

190. In Vitro Implementation of a Noise Filtering Gene Network

Pascal A. Pieters1, Ardjan J. van der Linden1, Jongmin Kim2, Peng Yin2, and Tom F.A.

de Greef1,3

(1)Department of Biomedical Engineering, Eindhoven University of Technology,

Eindhoven, Netherlands, (2)Wyss Institute for Biologically Inspired Engineering,

Harvard University, Boston, MA, (3)Institute for Molecules and Materials, Radboud

University, Nijmegen, Netherlands

191. CRISPR-Cas12 Mediated Genome-Editing in Oleaginous Yeas

Zhiliang Yang1, Harley Edwards2, and Peng Xu2

(1)Chemical, Biochemical and Environmental Engineering, University of Maryland,

Baltimore County, Baltimore, MD, (2)University of Maryland Baltimore County,

Baltimore, MD

192. Engineering Two Component Redox Sensors for Use in Marine Bacteria

Elizabeth Onderko1, Megalya Marquez-Lopez2, and Sarah Glaven3

(1)National Research Council, Washington, DC, (2)The Washington Center for

Internships and Academic Seminars, Washington, DC, (3)Center for Bio/Molecular

Science and Engineering, Naval Research Laboratory, Washington, DC

193. Building a Cas12a Toolkit to Facilitate Manipulation of the Transcriptome

James Bryson School of Biological sciences, University of Edinburgh, Edinburgh, United Kingdom

194. Melanin Biomaterials Produced By Engineered Bacteria

Chia-Suei Hung1, Zachary E. Reinert1,2, Maria Sanford1,2, Andrea R. Poole1,2, Cheri

Hampton1,2, Pamela Lloyd1,2, Annika Vaia1, Lawrence F. Drummy1, Matthew B.

Dickerson1, Wendy J. Crookes-Goodson1, Maneesh K. Gupta1, and Nancy Kelley-

Loughnane1

(1)Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-

Patterson Air Force Base, OH, (2)UES, Inc., Dayton, OH

195. Paper-Based, Cell-Free Detection of Heavy Metals for in-Field Water Quality

Testing

Casey B. Bernhards1, Kathryn Beabout2, Kendrick Turner3, Jorge L. Chávez4, Scott A.

Walper5, and Matthew Lux6

(1)CCDC Chemical Biological Center (Excet, Inc.), Aberdeen Proving Ground, MD,

(2)Integrative Health and Performance Sciences, UES, Inc., Dayton, OH, (3)NRL,

Washington, DC, (4)711th Human Performance Wing, Air Force Research Laboratory,

Wright-Patterson Air Force Base, OH, (5)Center for Biomolecular Science and

Engineering, Naval Research Laboratory, Washington, DC, (6)CCDC Chemical

Biological Center, Aberdeen Proving Ground, MD

196. Plasmid Vectors for In Vivo Selection Free Use with the Probiotic E. coli

Nissle

Anton Kan1, Ilia Gelfat2, Sivaram Emani3, and Neel Joshi4

(1)Wyss Institute, Harvard University, Boston, MA, (2)John A. Paulson School Of

Engineering And Applied Sciences, Harvard University, Cambridge, MA, (3)Harvard

University, Boston, MA, (4)Wyss Institute for Biologically Inspired Engineering, Harvard

University, Boston, MA

197. Cell-Free Systems for the Deployment of Biosensors in Resource-Limited

Environments

Steven Blum1, Glory Mgboji2, Aleksandr Miklos3, Peter Emanuel1, and Matthew Lux1

(1)CCDC Chemical Biological Center, Aberdeen Proving Ground, MD, (2)ORISE, Oak

Ridge, TN, (3)Excet, Inc., Springfield, VA

198. Engineering Ionotropic Chemogenetic Receptors in Yeast

Elizabeth Gardner1, Boris Zemelman2, Andrew Ellington1, and Jimmy Gollihar3

(1)Molecular Biosciences, University of Texas at Austin, Austin, TX, (2)University of

Texas Austin, Austin, TX, (3)US Army Research Laboratory- South, Austin, TX

199. Mosaic Polymers of the Plague Capsular Antigen (Caf1) Protein:

Engineering a Multi-Functional Biomaterial

Daniel T. Peters1, Gema Dura2, Helen Waller1, Adrian Yemm3, Neil D. Perkins3, David

A. Fulton2, Mark A. Birch4, and Jeremy H. Lakey1

(1)Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon

Tyne, United Kingdom, (2)School of Natural and Environmental Sciences, Newcastle

University, Newcastle upon Tyne, United Kingdom, (3)Institute for Cell and Molecular

Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom,

(4)Cambridge University, Cambridge, United Kingdom

200. The Impact of Genome Organization on the Regulation of Gene Expression

in E. coli

Anja Ehrmann1 and Morten H.H. Nørholm1,2

(1)Novo Nordisk Foundation Center for Biosustainability, Technical University of

Denmark, Kgs. Lyngby, Denmark, (2)Mycropt IVS, Copenhagen, Denmark

201. microRNA Mediated Endothelial Progenitor Function in Chronic Liver

Injury

Nan Wu1, Elise Slevin2, Heather Francis2, Gianfranco Alpini2, and Fanyin Meng1

(1)Roudebush VA Medical Center, Indiana Center for Liver Research, Indiana University

School of Medicine, Indianapolis, IN, (2)Indiana Center for Liver Research,

Indianapolis, IN

202. Reconstruction of Complex Fitness Landscapes Using High-Throughput

Experimental Data

Juannan Zhou and David McCandlish

Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring

Harbor, NY

203. Developing a Dynamic, High Affinity Scaffold Toolkit for the Control of

Intracellular Spatial Organization of Proteins and Metabolic Flux

Alexander Mitkas1 and Wilfred Chen2

(1)Chemical and Biomolecular Engineering, Universtiy of Delaware, Newark, DE,

(2)Chemical Engineering, University of Delaware, Newark, DE

204. Assembly of Synthetic Bacterial Microcompartments in Saccharomyces

Cerevisiae

Yong Quan Tan Biochemistry, National University of Singapore, Singapore, Singapore

205. Characterization of Gene Expression Regulated By Combined Action of a

Chemical Inducer and Temperature

Abhilash Patel, Soumyadip Banerjee, Pranjal Gupta, Nishant Kumar Sharma, and

Shaunak Sen

Electrical Engineering, IIT Delhi, New Delhi, India

206. Open-Source Paper-Fluidic Device for Bacterial Culture, Communication

and Biocomputation

Gonzalo A. Vidal-Peña1 and Tim Rudge1,2

(1)Institute for Biological and Medical Engineering, Schools of Engineering, Biology and

Medicine, Pontificial Catholic University of Chile, Santiago, Chile, (2)Department of

Chemical and Bioprocess Engineering, School of Engineering, Pontificial Catholic

University of Chile, Santiago, Chile

207. Noise-Reducing Optogenetic Circuits in Mammalian Cells

M. Tyler Guinn1 and Gabor Balazsi2

(1)Biomedical Engineering, Stony Brook School of Medicine, Stony Brook, NY,

(2)Biomedical Engineering, Stony Brook University, Stony Brook, NY

208. Application of Optimal Experimental Design to Omics Experimentation

Xiaokang Wang, Navneet Rai, Beatriz Pereira, Ameen Eetemadi, and Ilias Tagkopoulos

University of California-Davis, Davis, CA

209. DNA Construct Sequence Validation Using the Miseq Platform

Jonathan Diab1, Jennifer Chiniquy2, Zak Costello3, Tadeusz Ogorzalek1, Xiaoxian Liu4,

Mark Kulawik5, Cindi Hoover6, Joel M. Guenther7, Nurgul Kaplan1, Garima Goyal8,

Hector Plahar9, Brian Bushnell6, Samuel Deutsch10, and Nathan J Hillson1

(1)Technology Department, DOE Joint BioEnergy Institute, EMERYVILLE, CA, (2)DOE

Agile BioFoundry, EMERYVILLE, CA, (3)U.S. DOE Agile BioFoundry, Emeryville, CA,

(4)Joint BioEnergy Institute, Emeryville, CA, (5)Operations, Joint BioEnergy Institute,

Emeryville, CA, (6)Joint Genome Institute, Lawrence Berkeley National Laboratory,

Walnut Creek, CA, (7)Sandia National Laboratory, Livermore, CA, (8)Fuels Synthesis

Division, Joint BioEnergy Institute, Emeryville, CA, (9)Fuels Synthesis and Technology

Divisions, Joint BioEnergy Institute, Emeryville, CA, (10)DOE Joint Genome Institute,

Walnut Creek, CA

210. Design and Synthesis of RNA Thermoregulators to Control Metabolic Flux

in the Production of Essential Metabolites and Therapeutic Molecules

Jose Ruben Morones1 and Albert I Lerma Escalera2

(1)Centro de Investigación en Biotecnología y Nanotecnología, Universidad Autónoma

de Nuevo León, Apodaca, Nuevo León, NL, Mexico, (2)Centro de Investigacion en

Biotecnologia y Nanotecnologia, Universidad Autonoma de Nuevo Leon, Monterrey, NL,

Mexico

211. Improving Electron Flux in E. coli By Altering the Periplasmic Electron

Carriers

Lin Su1,2 and Caroline Ajo-Franklin2

(1)State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China, (2)The

Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA

212. Engineering Transcriptional Interference for NAND and NOR Logic

Behaviors

Nolan O'Connor, Antoni E. Bordoy, and Anushree Chatterjee

Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO

213. Novel Cell-Free Protein Synthesis System of Chitinases from Serratia

Marcescens DB11

Michael Huang1, Jayashree Chakravarty2, Christopher Brigham3, and Tracie Ferreira4

(1)Bioengineering, University of Massachusetts Dartmouth, North Dartmouth, MA,

(2)Bioengineering, University of Massachusetts Dartmouth, NORTH DARTMOUTH,

MA, (3)Interdisciplinary Engineering, Wentworth Institute of Technology, Boston, MA,

(4)University of Massachusetts Dartmouth, NORTH DARTMOUTH, MA

214. Isolating Live Cells after High-Throughput, Long-Term, Time-Lapse

Microscopy

Scott Luro Systems Biology, Harvard Medical School, Boston, MA

215. Engineering Synthetic Biotic MedicinesTM for the Treatment of Cancer

Ning LI, Daniel Leventhal, Kip West, Chris Plescia, Adam Fisher, Carey Gallant,

Starsha Kolodziej, Rudy Christmas, Anna Sokolovska, Michael James, Mary Castillo,

Paul Miller, and Jose Lora

Synlogic Inc., Cambridge, MA

216. Minimal Genomes: In-Silico using Whole-Cell Models, in-Vivo using

CRISPR-cas9

Joshua Rees Life Sciences, University of Bristol, Bristol, United Kingdom

217. Synthetic Biology Platform for Modifying Glycosylation of

Biopharmaceuticals

Christopher Stach1, Meghan G. McCann2, Conor O’Brien3, Tung S. Le4, Wei-Shou Hu2,

and Michael J. Smanski5

(1)Department of Biochemistry, Molecular Biology, and Biophysics, University of

Minnesota, Twin Cities, Minneapolis, MN, (2)Department of Chemical Engineering and

Materials Science, University of Minnesota, Twin Cities, Minneapolis, MN,

(3)Department of Chemical Engineering and Materials Science, University of Minnesota,

Minneapolis, MN, (4)Chemical Engineering and Materials Science, University of

Minnesota, Twin Cities, Minneapolis, MN, (5)Department of Biochemistry, Molecular

Biology, and Biophysics, University of Minnesota, Twin Cities, St. Paul, MN

218. Analysis of Macromolecular Crowding Effects on Gene Expression in E. coli

Cell-Free Systems

Ti Wu Chemistry and Biochemistry, UC San Diego, La Jolla, CA

219. Improving Energy Supplies in E. coli Cell-Free Systems with Polyphosphate

Kinase 2

Ti Wu Chemistry and Biochemistry, UC San Diego, La Jolla, CA

220. Data-Driven Cellular Capacity Optimization through Proteome Reclamation

Robert Egbert Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA

221. An Automated Droplet-Based Microfluidic System for Optimizing

Biosynthetic Pathways

Kosuke Iwai1,2, Maren Wehrs3, Peter W. Kim1,2, Trent Northen1,4, Aindrila

Mukhopadhyay3,5, Hector Garcia-Martin3,5, Paul D. Adams1,6,7, and Anup K Singh1,8

(1)Technology Division, Joint BioEnergy Institute, Emeryville, CA, (2)Biotechnology and

Bioengineering Department, Sandia National Laboratories, Livermore, CA, (3)Biological

Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA,

(4)Environmental Genomics and Systems Biology Division, Lawrence Berkeley National

Laboratory, Berkeley, CA, (5)Biofuels and Bioproducts, Joint BioEnergy Institute,

Emeryville, CA, (6)Department of Bioengineering, University of California, Berkeley,

Berkeley, CA, (7)Molecular Biophysics and Integrated Bioimaging Division, Lawrence

Berkeley National Laboratory, Berkeley, CA, (8)Biological and Engineering Sciences,

Sandia National Laboratories, Livermore, CA

222. Engineering an Orthogonal DNA Replication System for Sexual

Recombination and Biosensor Evolution

Alex Javanpour Biomedical Engineering, UC Irvine, Irvine, CA

223. Design of a Layered Feedback Controller in Biological Systems

Chelsea Hu and Richard M. Murray

Division of Biology and Biological Engineering, California Institute of Technology,

Pasadena, CA

224. Synthetic Genetic Incompatibility; dCas9-Based Programmable

Transcription Activation for Invasive Species Biocontrol and Transgene

Biocontainment

Samuel Erickson1, Siba Das2, and Michael J. Smanski3

(1)BioTechnology Institute, University of Minnesota, Saint Paul, MN, (2)University of

Minnesota, Saint, MN, (3)Department of Biochemistry, Molecular Biology, and

Biophysics, University of Minnesota, Twin Cities, St. Paul, MN

225. Bioswitches and Robots for Systems Metabolic Engineering and Synthetic

Biology of Hyper Microbial Production Strains

Lifu Song1,2, Uwe Jandt2, Lin Chen2, Jie Ren1, Libang Zhou1, and An-Ping Zeng1,2

(1)Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing

University of Chemical Technology, Beijing, China, (2)Institute of Bioprocess and

Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany

226. Emerging Technologies and Frontiers of Biomolecular Engineering:

Combining Rational Design, Machine Learning and Automated Experimental

Feedback

Uwe Jandt1 and An-Ping Zeng1,2

(1)Institute of Bioprocess and Biosystems Engineering, Hamburg University of

Technology, Hamburg, Germany, (2)Beijing Advanced Innovation Center for Soft Matter

Science and Engineering, Beijing University of Chemical Technology, Beijing, China

227. Orthogonal Transmembrane Chemical Signalling across Synthetic Cells

Yujia Qing1 and Hagan Bayley2

(1)Chemistry, University of Oxford, OXFORD, United Kingdom, (2)Chemistry,

University of Oxford, Oxford, United Kingdom

228. Identification and Characterization of Arabinoxylanases from a Newly

Isolated Thermophilic Bacterium to Improve Fermentation of Corn Fiber

Dhananjay Beri1, Lee R. Lynd2, William S. York3, Maria Pena3, and Christopher D.

Herring4

(1)Thayer school of Engineering, Dartmouth College, Hanover, NH, (2)Thayer School of

Engineering, Department of Biological Sciences, Dartmouth College, Hanover, NH,

(3)Complex Carbohydrate Research Center, University of Georgia, Athens, GA, (4)Enchi

Corporation, Waltham, MA

229. Genome Mining and Strain Engineering to Identify Novel Drugs from

Filamentous Fungi

Nadia Chacko, Venkatesh Endalur Gopinarayan, Cindy Guo, Colin Lazzara, Paul

Secrist, Gregory Verdine, and Philippe Prochasson

LifeMine Therapeutics, Cambridge, MA

230. Pairing Genome Engineering of Synthetic Yeast Strains with High

Throughput Screening for Enhanced Flavonoid Production

Jamie Auxillos University of Edinburgh, Edinburgh, United Kingdom; University of Manchester,

Manchester, United Kingdom

231. Evaluation of Engineered Microbe Persistence and Function of Using a

Simplified Polymicrobial Gut Community

Steven Arcidiacono CCDC-Soldier Center, Natick, MA

232. Microbial Dynamic during Start-up Process of Anaerobic Digestion of

Sugarcane Vinasse

Licelly Canizalez-González1, Edgar Blanco2, John Ward3, and Maria F Villegas-Torres4

(1)Universidad Icesi, Cali, Colombia, (2)Anaero Technology, Cambridge, United

Kingdom, (3)Department of Biochemical Engineering, University College London,

London, United Kingdom, (4)Universidad Icesi, CALI, Colombia

233. Biotechnological Applications for Light-Regulated Cre Recombinase

Lena Hochrein1, Leslie A. Mitchell2, and Bernd Mueller-Roeber1

(1)Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany,

(2)Institute for Systems Genetics, NYU Langone Health, New York, NY

234. Engineered Microbes for Therapeutic Applications

Chun Loong Ho Department of Biomedical Engineering, Southern University of Science and Technology,

Shenzhen, China

235. Learning from Evolutionary Biology to Engineer Crop Plants for the Future

Prem Bhalla and Mohan Singh

Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville,

Australia

236. Toward a Translationally-Independent Guide RNA Oscillator

James Kuo1,2, Ruoshi Yuan1, Johan Paulsson1, and Pamela A. Silver1,2

(1)Systems Biology, Harvard Medical School, Boston, MA, (2)Wyss Institute for

Biologically Inspired Engineering at Harvard University, Boston, MA