enhanced hydrocarbon bioremediation through the addition of humic … › wp-content › uploads ›...
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ENHANCED HYDROCARBON BIOREMEDIATION THROUGHENHANCED HYDROCARBON BIOREMEDIATION THROUGHTHE ADDITION OF HUMIC SUBSTANCESTHE ADDITION OF HUMIC SUBSTANCES PART II: PART II:
IMPROVED LAND TREATMENT AND STATIC-PILE COMPOSTINGIMPROVED LAND TREATMENT AND STATIC-PILE COMPOSTING
L. E. Liem and R. M. Facey (Hood Environmental, Sherwood Park)L. E. Liem and R. M. Facey (Hood Environmental, Sherwood Park)
M. Fischer (Luscar Specialty Products, Edmonton)M. Fischer (Luscar Specialty Products, Edmonton)
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RATIONALERATIONALE
� Potential hydrocarbon
contamination in soil
� Humic substances improve soil
quality � enhancing hydrocarbon
biodegradation
� Excellent potential market over
landfilling
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SCOPE OF WORKSCOPE OF WORK
� Improved performance of humic
substances in enhancing
hydrocarbon biodegradation �bulking agents and soil additives
addition
� Laboratory experiments of land
treatment and static-pile
composting
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GUIDELINESGUIDELINES
� Canadian Council of Ministries of
the Environment (CCME)
� Tier I for Petroleum Hydrocarbons
(PHC)
� Agricultural application for fine
grain soil
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TREATMENT ALTERNATIVESTREATMENT ALTERNATIVES
� Land treatment: lower capital &
operation, less optimized process
� Static-pile composting: higher
capital & operation, more optimized
process
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LAND TREATMENTLAND TREATMENT
� 300 mm depth
� Manual tilling
� Mesophilic
� C:N = 20 to 25:1
� 50 to 75% field capacity
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STATIC-PILE COMPOSTINGSTATIC-PILE COMPOSTING
� 2 to 3 m depth
� Forced aeration
� Thermophilic
� C:N = 20 to 25:1
� 50 to 75% field capacity
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SOIL PROPERTIESSOIL PROPERTIES
7.3-pH
85%Field Capacity
1,100#/gHPC
0.34%Total Nutrient
2.4%Total Carbon
15%Sand
22%Silt
63%Clay
1,000kg/m3Bulk Density
50%Porosity
23%Moisture
ValueUnitParameter
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HYDROCARBON CONTAMINANTHYDROCARBON CONTAMINANT
� Diesel: 97% C7 to C
22 @ 36.1 to
503.0ºC
� Evaporation < 0.1% @ 36 to 69 ºC
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HYDROCARBON BIODEGRADATIONHYDROCARBON BIODEGRADATION
� Improve soil quality
� Increase hydrocarbon solubility in
soil
� Enhance growth of soil
microorganisms
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HUMICHUMIC SUBSTANCES SUBSTANCES
� Provide available carbon to soil
� Maintain soil pH around neutral
� Increase solubility of
hydrocarbons in soil
� Improve water retention in soil
� Dissolve clay aggregate
� Retain micronutrients in soil
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HUMICHUMIC SUBSTANCES (Cont SUBSTANCES (Cont’’d)d)
17%
83%
micron
micron
Particle Size
75 to 180
< 75
866 to 965kg/m3Bulk Density
7.0 to 7.4-pH
55%Organics
ValueUnitParameter
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BULKING AGENTSBULKING AGENTS
� Improve soil porosity
� Provide available nutrients
� Increase initial number of
microorganisms
� Provide medium for microorganisms
growth
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BULKING AGENTS (ContBULKING AGENTS (Cont’’d)d)
250E6
7.2
0.94
18.2
18 (> 4 mm)
57 (0.5 to 4 mm)
25 (< 0.5 mm)
585
50
29
Compost
7.1-pH
%Field Capacity
77E6#/gHPC
0.88%Total Nitrogen
38.7%Total Carbon
39 (> 4 mm)
53 (0.5 to 4 mm)
8 (< 0.5 mm)
%
%
%
Particle Size
250kg/m3Bulk Density
50%Porosity
62%Moisture
Wood-MulchUnitParameter
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SOIL ADDITIVESSOIL ADDITIVES
� Improve hydrocarbon degradation
� Enhance microorganisms growth
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SOIL ADDITIVES (ContSOIL ADDITIVES (Cont’’d)d)
60
30
Biostimulants:
Bacterial culture
Nutrients
10Extra-cellular enzymes
% (by weight)Component
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EXPERIMENTAL DESIGN:EXPERIMENTAL DESIGN:Land TreatmentLand Treatment
� Diesel: 0.25 to 0.5 % by weight
� Humic substances: 0 and 5% by
weight
� Bulking agents: 0 and 50% by
weight
� Soil additives: 0 and 0.1% by weight
� Temperature: ambient @ 20 °C
� Aeration: tilling
� Moisture: 50% by weight
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EXPERIMENTAL DESIGN:EXPERIMENTAL DESIGN:Static-Pile CompostingStatic-Pile Composting
� Diesel: 0.25 to 0.5 % by weight
� Humic substances: 0 and 5% by
weight
� Bulking agents: 0 and 50% by
weight
� Temperature: heated to 50 °C
� Aeration: forced
� Moisture: 50% by weight weekly
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LABORATORY EQUIPMENTLABORATORY EQUIPMENT
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MONITORING & SAMPLINGMONITORING & SAMPLING
� Moisture contents: weekly
� Soil aggregate size: weekly
� Total C & N, HPC, pH, TEH: tri-
weekly
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MOISTURE RETAININGMOISTURE RETAINING
Soil Mixture BeforeAdjustment
(%)
AfterAdjustment
(%)
MoistureRetaining
(%)
Soil 66(8.4) 48(11.4) 72(11.7)
Soil+bulking agents 71(10.0) 49(12.0) 68(11.5)
Soil+humic (1%)Soil+humic (5%)
64(7.5)60(6.7)
50(9.8)53(8.4)
78(10.0)87(9.2)
Soil +humic(1%)+bulking agentsSoil+humic(5%)+bulking agents
66(9.5)62(7.0)
50(10.0)54(8.1)
75(10.5)86(7.2)
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AGGREGATE SIZEAGGREGATE SIZE
Soil Mixture Aggregate Size Distribution (%)
≥ 4.0 mm 2.4 to 4.0mm
1.4 to 2.4mm
Soil 88(4.9) 5(1.7) 2(3.20
Soil+bulking agents 76(3.9) 11(8.8) 4(3.4)
Soil+humic (1%)Soil+humic (5%)
84 (3.4)70 (11.2)
6 (1.9)13 (4.5)
3 (1.2)6 (3.8)
Soil+humic(1%)+bulking agentsSoil+humic(5%)+bulking agents
73(9.7)75(8.7)
14(6.9)13(4.4)
6(3.1)4(3.0)
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pH & C:NpH & C:N
Soil Mixture Initial Week Values
pH C:N
Soil 7.6 7
Soil+bulking agents 7.4 14
Soil+humic (1%)1
Soil+humic (5%)17.47.2
914
Soil+humic (1%)+bulking agentsSoil+humic (5%)+bulking agents
7.47.2
1721
Soil+additives (enzymes)Soil+additives (enzymes+biostimulants)
7.4 - 7.57.4 - 7.5
13 - 1413 - 14
Soil+humic(1%)+additives(enzymes)Soil+humic(5%)+additives(enzymes+biostimulants)
7.1 - 7.47.0 - 7.2
17 - 1916 - 24
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HPC PROFILESHPC PROFILES
HPC Profiles (Bulking Agents and Humic Material)
0
100
200
300
400
500
0 3 6 9 12Weeks
HP
C (
10e6
/g) Control
Humic (5%)
Bulking Agents
BulkingAgents+Humic(5%)
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HPC PROFILES (ContHPC PROFILES (Cont’’d)d)
HPC Profiles (Soil Additives and Humic Material)
0
100
200
300
400
500
0 3 6 9 12Weeks
HP
C (
10
e6
/g)
Control
Humic(5%)
Enzymes
Enzymes+Biostimulants
Enzymes+Humic(5%)
Enzymes+Biostimulants+Humic(5%)
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HPC PROFILES (ContHPC PROFILES (Cont’’d)d)
HPC Profiles (Composting at 50 'C)
0
100
200
300
400
500
0 3 6 9 12Weeks
HP
C (
10
e6
/g)
Manual Tilling
Manual Tilling + Humic (5%)
Forced Aeration
Forced Aeration+Humic(5%)
Manual Tilling + Bulking Agents
Manual Tilling + Bulking Agents +Humic(5%)Forced Aeration + Bulking Agents
Forced Aeration + Bulking Agents +Humic (5%)
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TEH PROFILESTEH PROFILES
TEH (Bulking Agents and Humic Material)
0
1000
2000
3000
4000
5000
0 3 6 9 12Weeks
TE
H (
mg/
kg)
Control
Humic (1%)
Humic (5%)
Bulking Agents
Humic (1%) + Bulking Agents
Humic (5%) + Bulking Agents
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TEH PROFILES (ContTEH PROFILES (Cont’’d)d)
TEH (Soil Additives + Humic Material)
0
1000
2000
3000
4000
5000
0 3 6 9 12Weeks
TE
H (
mg/
kg)
Control
Humic (5%)
Enzymes
Enzymes+Biostimulants
Enzymes+Humic (5%)
Enzymes+Biostimulants+Humic(5%)
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TEH PROFILES (ContTEH PROFILES (Cont’’d)d)
TEH (Composting at 50 'C)
0
1000
2000
3000
4000
5000
0 3 6 9 12Weeks
TE
H (
mg/
kg)
Tilling + Control
Tilling + Humic (5%)
Aeration + Control
Aeration + Humic (5%)
Tilling + Bulking Agents
Tilling + Bulking Agents + Humic(5%)Aeration + Bulking Agents
Aeration + Bulking Agents +Humic (5%)
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LAND TREATMENTLAND TREATMENTSUMMARYSUMMARYNo Treatment Tier I Objective
(800 mg/kg)
1 Control 3,300 mg/kg @ 12 weeks
2 Humic @ 1% 1,200 mg/kg @ 12 weeks
3 Humic @ 5% Tier I @ 9 to 12 weeks
4 Bulking Agents @ 50% 1,000 mg/kg @ 12 weeks
5 2 + 4 Tier I @ 12 weeks
6 3 + 4 Tier I @ 6 to 9 weeks
7 Soil additives (enzymes) @ 0.1% 1,000 mg/kg @ 12 weeks
8 Soil additives (enzymes+biostimulants)@ 0.1% Tier I @ 12 weeks
9 3 + 7 Tier I @ 6 to 9 weeks
10 3 + 8 Tier I @ 3 to 6 weeks
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STATIC-PILE COMPOSTINGSTATIC-PILE COMPOSTINGSUMMARYSUMMARYNo Treatment Aeration Tier I Objective
(800 mg/kg)
1 Control Tilling 2,700 mg/kg @ 12 weeks
2 Control Aeration 1,600 mg/kg @ 12 weeks
3 Humic @ 5% Tilling Tier I @ 6 to 9 weeks
4 Humic @ 5% Aeration Tier I @ 6 to 9 weeks
5 Bulking Agents @ 50% Tilling Tier I @ 9 to 12 weeks
6 Bulking Agents @ 50% Aeration Tier I @ 9 to 12 weeks
7 3 + 5 Tilling Tier I @ 6 to 9 weeks
8 4 + 6 Aeration Tier I @ 3 to 6 weeks
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TREATMENT ALTERNATIVESTREATMENT ALTERNATIVESSUMMARYSUMMARY
� Forced aeration outperformed tilling
� Higher temperature was better
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HUMIC SUBSTANCESHUMIC SUBSTANCESSUMMARYSUMMARY
� Improve soil quality
� Enhance microorganisms growth
� Enhance diesel biodegradation
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BULKING AGENTS & SOIL ADDITIVESBULKING AGENTS & SOIL ADDITIVESSUMMARYSUMMARY
� Improve humic substances performance
� Enhance microorganisms growth anddiesel biodegradation
� Optimum dosages to be determinedmore thoroughly
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ENHANCED BIODEGRADATIONENHANCED BIODEGRADATIONSUMMARYSUMMARY
� Shorter timeline for Tier I
� Simpler operation & optimization
� Cost factors to be considered
� Different hydrocarbons to be tested