environmental express webinar method 1664 – oil and grease … · 2016-02-03 · from epa 413.1...

Environmental Express WebinarEnvironmental Express WebinarMethod 1664 –

Oil and Grease LightningOil and Grease Lightning

02/02/2016

What is Oil & Grease?What is Oil & Grease?

Method-Defined AnalyteMethod Defined Analyte

HEM vs. Oil & Grease Method modifications are extremely limited Method modifications are extremely limited Modifications cannot alter the chemistry of the

method Modifications cannot adversely affect HEM

recoveries

From EPA 413.1 to EPA 1664

Replaced Freon with n-hexane 1664 d t d i 1999 d t

From EPA 413.1 to EPA 1664

1664 adopted in 1999 and we are now up to Revision B

EPA withdrew all Freon methods from 40 CFR EPA withdrew all Freon methods from 40 CFR part 136 in 2007

1664 is now the only method approved for y pptesting wastewater for oil & grease

What did Method Change Mean?What did Method Change Mean?

Oil & Grease still a “method-defined analyte” though is id d H E t t bl M t i l (HEM)now considered Hexane Extractable Material (HEM)

The term "total petroleum hydrocarbons" (TPH) has been replaced by "non-polar material" (NPM) to avoid replaced by non polar material (NPM) to avoid confusion with TPH measurements by other methods

The requirement for a matrix spike duplicate (MSD) has b h d ibeen changed to a suggestion

The size of an analytical batch has been increased to a maximum of 20 samples per discharge or waste streammaximum of 20 samples per discharge or waste stream

Use of solid-phase extraction (SPE) has been allowed without a demonstration of equivalency

LLE vs. SPELLE vs. SPE

Liquid-Liquid Extraction Solid-Phase Extractionq q

3 shakes of 30 mL of solvent

Little solvent usage (as little as 40 – 50 mL)solve t

Higher analyst exposure to n-hexane

l ttle as 0 50 ) Less solvent = less

exposure Prone to emulsions Relies on analyst

Less labor intensive Frees up analysts to do

precision and accuracy Hexane floats!

other things in the lab More consistent results

Solid Phase Extraction (SPE)Solid Phase Extraction (SPE)

SPE allowed in Revision A of Method 1664 without a demonstration of equivalency

Utilizes an extraction disk impregnated with C-18 as the absorbent resinabsorbent resin

The C-18 in the disk is “activated” with a polar solvent (methanol) prior to sample filtrationS l i ll d th h th di k hi h t i Sample is vacuum pulled through the disk which retains the HEM

HEM is then eluted off of the disk with n-hexane Hexane + HEM collected in a pre-weighed flask or dish

and the n-hexane driven off by heat to leave the HEM behindbehind

Important things about SPEImportant things about SPE

Extraction disc is not analyte yspecific

C-18 used to make discs will adsorb antibiotics, pesticides, herbicides, surfactants, and vitamins

Solvent used for elution is the determining factor

Capacity of the disc –‘parking lot’ analogy

Manual SPEManual SPE

Assembling the StepSaverAssembling the StepSaver

Using the StepSaverUsing the StepSaver

Disc Activation – Alternate option, UltraPrepoption, UltraPrep

Remove methanol, replacewith DI waterwith DI water

Vacuum ‘filter’ sample

Dry disc

El t ith h Elute with hexane

Evaporate the hexane

Potential Problems with SPE

Particulate laden samples

Potential Problems with SPE

Extraction disc activation

Residual analyte in the sample bottle

Automated SPEAutomated SPE

Environmental Express SKUs -- Equipment StepSaver Systems StepSaver Systems

Part # DescriptionG1047 1 Station 47mm w/ Glass Funnel

Part # DescriptionG1121 1 Station PVC Disposable FunnelG1047 1 Station. 47mm w/ Glass Funnel

G1090 1 Station. 90mm w/ Glass Funnel

G5747 1 Station. 47mm no funnel

G1101 1 Station. PVC. Glass Funnel


G1121 1 Station. PVC. Disposable Funnel



G2121 1 Station. Stainless Steel. Disposable Funnel



G2101 1 Station. Stainless Steel. Glass Funnel



GUP1101 1 Station PVC Glass Funnel With UltraPrep® funnels


GUP1121 1 Station. PVC. Disposable Funnel With UltraPrep® Funnels



GUP2121 1 St ti St i l St l Di bl F l With Ult P F lGUP1101 1 Station. PVC. Glass Funnel. With UltraPrep® funnels

GUP1103 3 Station. PVC. Glass Funnel. With UltraPrep® funnels

GUP1107 7 Station. PVC. Glass Funnel. With UltraPrep® funnels

GUP2101 1 Station. Stainless Steel. Glass Funnel. With UltraPrep® funnels


GUP2121 1 Station. Stainless Steel. Disposable Funnel With UltraPrep® Funnels




Environmental Express SKUs -- DiscsEnvironmental Express SKUs Discs

Part # DescriptionG5300MM UltraLow™ – 47mm C18 discs without prefilters. 20pkp p

G5347MM UltraLow™ – 90mm C18 discs without prefilters. 15pk

G5390MM UltraLow™ – 100mm C18 discs without prefilters. 15pk

G5047MM UltraFlow™ – 47mm C18 discs with prefilters. 20pk



G5127 StepSaver disposable funnels with 47mm UltraFlow™ discs. 20pk

G5260 Filter assembly with UltraFlow™ 47mm disc. No Funnel. 100pk

GUP047MM UltraPrep® – 47mm Preactivated discs with prefilters. 20pk



GUPF47EE StepSaver disposable funnels with 47mm UltraPrep® discs. 20pk

FG650100MM 100mm Thick glass fiber prefilters. 50pk

FG65041MM 41mm Thick glass fiber prefilters 50pkFG65041MM 41mm Thick glass fiber prefilters. 50pk



Environmental Express SKUs -- AccessoriesEnvironmental Express SKUs Accessories

Part # DescriptionG2018 Standard 5mL tubes 40mg HEM 20pk

Part # DescriptionLCCC08002 Reagent n-Hexane 2 5LG2018 Standard. 5mL tubes. 40mg HEM. 20pk

G3004 Standard. 10mL tubes. 4mg HEM. 20pk

G3020 Standard. 30mL bottles. 40mg HEM. 9pk

G3023 MDL Standard. 10mL tubes. 7mg HEM. 20pk


LCCC08002 Reagent. n Hexane. 2.5L

LCCC08002C Reagent. n-Hexane. 4x 2.5L

LCCC08003 Reagent. Methanol ACS. 2.5L

LCCC08003C Reagent. Methanol ACS. 4x2.5L

LCCC08004 Deionized Water. 2.5L

G3025 Standard. 10mL tubes. 40mg HEM. 20pk


G7000 QuikVap evaporating system. 120 volt

G7000-240 QuikVap evaporating system. 240 volt

LCCC08004C Deionized Water. 4x2.5L

LCCC08005 Reagent. n-Hexane. For smart bottle rack system. 4L

LCCC08005C Reagent. n-Hexane. For smart bottle rack system. 4x4L

LCCC08006 Reagent. Methanol ACS. For smart bottle rack system. 1L

G1200 Single-place stainless steel manifold for Oil & Grease

G1210 Single-place PVC manifold for Oil & Grease

G1250 3-Place stainless steel manifold

G1260 3-Place PVC manifold for Oil & Grease

G1270 7 Pl PVC if ld f Oil & G

LCCC08006C Reagent. Methanol ACS. For smart bottle rack system. 6x1L

LCCC08007 Deionized water for smart bottle rack system. 1L

LCCC08007C Deionized water for smart bottle rack system. 6x1L

G1270 7-Place PVC manifold for Oil & Grease

G1300 6-Place stainless steel manifold for Oil & Grease

G3050 1/8 HP vacuum pump. 25.5” Hg. 115VAC

Thank you for attendingThank you for attendingQuestions?

Email us at [email protected] OR call us 800.343.5319http://www.envexp.com/products/1-Wet_Chemistry/SS-Oil_and_Grease_EPA_1664

02/02/2016

p p p y

environmental express webinar method 1664 – oil and grease … · 2016-02-03 · from epa 413.1...

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