Enzymes for Biocatalysis

By Dr. Bhalchandra Vaidya

Introduction

• Enzymes are catalytic proteins from biological

sources.

• Enzymes accelerate the rate of reaction by

lowering the energy of activation.

• Enzymes can be obtained from plants, animals or

micro-organisms

Enzyme Technology

• An emerging field of biotechnology that utilizes

enzymes for several commercial applications.

– Pharmaceuticals

– Fine chemicals

– Foods and beverages

– Cosmetics

– Textile

– Paper

– Leather

– Animal Feed

Goals of Enzyme technology

• Integration of biology, chemistry and engineering

• To develop of novel alternative routes, processes

and services

• To design innovative products and processes that

are not only competitive but also meet criteria of

sustainability

Advantages of enzymes

• Efficient catalysis

• Selective (Enantio- and regio-selectivity)

• Specific in action (Less byproducts)

• Environmentally benign catalysis

– Non Toxic, Non- flammable,

– Completely biodegradable,

– Sustainable,

– Requires mild reaction conditions

• Recycled and reused when immobilized

Misconceptions and myths

• Enzymes are highly unstable

• Enzymes act only on natural substrates

• Enzymes are too expensive

• Productivity of Enzymes are too low

Enzymes in Pharma Industry

• Chirality plays a major role in design,

development, marketing and patenting of APIs.

• Being stereo-selective, enzymes offer specific

solutions to introduce chirality in APIs.

Classification of enzymes

Classification of enzymes

Hydrolases

• Hydrolysis of C-O, C-N, C-C bonds

• Favorite class of Enzymes for organic chemistry

– No need of co-factor

– Reversible in action

– Wide substrate specificity and catalytic versatality

– Relatively higher stability in non-natural conditions

• Large number of hydrolases are commercially

available

• Extensively employed for industrial processes

Enzymes (hydrolases) for

Biocatalysis by Advanced Enzymes

• Lipases

– CAL-A (Candida antarctica lipase-A)

– CAL-B (Candida antarctica lipase-A)

– TLL (Thermomyces lanuginosa Lipase)

– RDL (Rhizopus delemar lipase)

– ANL (Aspergillus niger lipase)

• Esterases

• Proteases

• Amylase and other carbohydratases

Lipase

A Versatile biocatalyst

Lipase

A Versatile biocatalyst

Lipases are used for:

• Resolution of carboxylic acids

• Resolution of alcohols

Lipases are used for:

• Synthesis of enantiopure amides:

• Desymmetrization reactions:

Lipases are used for

• Synthesis of enantiopure epoxides

What we offer…

• We at Advanced Enzymes are manufacturing,

marketing and exporting enzymes for the various

applications over the last 50 years.

• Our technical and application labs are well

equipped to develop enzymatic processes and to

provide technical solutions.

• Talk to us for the new development and support.

Concluding remarks

• Sustainability is development that meets the needs

of the present without compromising the ability of

future generations to meet their own needs.

• Enzymes technology is an invaluable tool for organic

chemists to create the future in balance between

economy, cleaner environment and better lives

Thank You

“Think of Life …

Think of enzymes…

Think of Advanced Enzymes”

Where Enzyme is life