Enzymes for Environmental Sustainability and Maximizing Profits

For more than ten years, Novozymes has been recognized as one of the most sustainable companies in the world by the Dow Jones Sustainability Index (DJSI), which evaluates and ranks leading companies based on their sustainability performance.

Sustainability – Novozymes commitment

Agenda

1. NOVOZYMES – IN BRIEF

2. OUR PRESENCE IN DISTILLERY

3. LIQUEFACTION PRODUCTS

4. SACCHARIFICATION PRODUCTS

5. PROCESS AIDS & ENHANCER PRODUCTS

6. BENEFITS DERIVED

7. SERVICES OFFERED

Everywhere you need us

Your needs?

More food and

better farming

Cleaner, smarter,

safer production

Economic growth

and job creation

Sustainable cities

and waste recycling

Clean air and

water

Renewable energy A world in

balance

Global consumer

preference

Novozymes is driving the change

to greener fuel and renewable

energy.

Our unique enzymes break

down plants and plant waste

to create ethanol. We increase

plant profitability for both starch-

based and cellulosic ethanol

production and enable new

energy industries.

Plugging into the power of plants

Bioenergy Novozymes

rethinks fuel:

Renewable energy

Less dependence

on fossil fuels

Less CO²

Greater energy security

Conversion of organic

waste

New jobs

Value-chain benefits –creating food, animal

feed and renewable

materials together with

ethanol

Novozymes’ R&D

Novozymes’ R&D innovates

closely with customers and

partners to create superior

results. By investing 14%

of our revenue in R&D,

Novozymes stays on the

cutting edge of innovation.

Our worldwide R&D

community allows us to

develop solutions that meet

both global and local needs.

Novozymes’ R&D: • creates impact for our

customers

• advances industries

• paves the way for a

brighter future

What we do in distillery

Liquefaction Fermentation Distillation Effluent

Treatment

Specialized Products Omnipresent

Specialized Products

Plants are designed to use - and gluco-

amylase but other enzymes may be required due to:

- Feedstock differences - Efficiency improvement - Energy optimization - Yield improvement

Reduced fouling

Viscosity reduction

Running higher solids

Improved fermentation performance

GLUCO-AMYLASE

PROTEASE & PHYTASE

UREA

PROTEASE

ENZYMES AS PROCESSING AIDS

Reduced

fouling

Improved

separation

DRIED DISTILLER GRAINS WITH SOLUBLES

(DDGS)

Improved feed

quality

What the industry wants today….

Hig

h

• Robust process

• Low down time

• Ease of operation

• Efficiency

• Product Quality

Inp

ut

to P

rocess

• Energy (steam)

• Water usage

• Chemical usage

• Effluent generated

• Alcohol generated in fermented mash

• High throughput from existing plant

Lo

w

Low

Output from process

High

Liquefaction

Viscosity

Reduction

01

No jet cooking

03

Optimum

dextrinization

02

Low pH cooking

04

Yield Gain

05

3 + 4 + 5

clubbed

together

06

LIQUEFACTION PRODUCTS

Liquoflow, Liquoflow Basic

• Patented, protein-engineered technology

• Low calcium dependency – doesn’t require add-on’s to improve stability

• Broad pH activity range (pH 5.5 – 6.0)

Liquoflow 4X

• Same technology as Liquoflow

basic & Liquoflow

• More concentrated formulation offers better value

• Ideal choice for large users or overcoming plant limitations

Novozym 25081

• Low pH AA

• Can work as low as 4.7

• Reduces / Eliminates requirement

of alkali in cooking pH adjustments

• Enables higher recycle of thin slop

• Creates safe working environment

Avantec

• Side activity of Gluco-amylase & Protease

• ~2.0% yield gain vs traditional AA’s

• pH profile from 4.8 – 5.8

• Significant energy and chemical savings

• In launch phase in India

High yield Industry Workhorse

Fermentation

Dextrin's to

Glucose

01

Faster

fermentation

process

03

Low residual

starch

02

Yeast nutrition

04

Hydrolyze

difficult starch

05

High yield

06

SACCHARIFICATION PRODUCTS

Saczyme

• Consistent fermentation performance

• Proven track record

Saczyme Plus 2X

• Best-selling GA on the market today

• Optimized component activities for

more complete starch conversion

• Consistently high ethanol yields

Saczyme Pro

• Converts the most difficult starch

fractions

• Most robust performance for

variable mash conditions

• High ethanol yields

Saczyme Yield

• ~1.5% yield gain vs traditional GA’s & 3% energy savings.

• Yield gain from bound starch freed

by cellulase & not from fiber

degradation to Glucose

• In launch phase in India

High yield Industry Workhorse

Access more Starch

0.0%

0.5%

1.0%

1.5%

2.0%

2.5%

3.0%

Yield increase with

Saczyme® Yield

• New enzyme activities release bound

starch from the grain fiber matrix

• 1%-2.5% increases in yield are being

observed in field trials

Industrial scale plant trials with Saczyme Yield

Other glucoamylases hydrolyze

starch on the surface, but cannot

access starch bound in the fiber

matrix.

Saczyme Yield degrades cell walls

to access more starch, allowing for

greater conversion and higher

yields.

Treatment of corn with a traditional

glucoamylase

Saczyme Yield under high magnification Treatment of corn with Saczyme Yield

Traditional glucoamylase under high

magnification

Saczyme Yield degrades fiber to access more starch

VISCOSITY REDUCING ENZYMES (VRE)

A cocktail of enzymes specific for the grain.

Typically includes a formulation of:

• Xylanases

• Cellulases

• Beta-glucanases

Guess which sample has been treated

with Viscosity Reducing Enzymes?

PROTEASE MECHANISM

Proteases for ethanol production have both endo- and exo-activities that contributes to faster amino acid release by cleaving protein in multiple sites

A protease will have a specific amino acid sequence it will recognize and cleave

Reduces complexity of proteins

-

2

ASN

CYS LEU

TYR

ASN

PRO

GLU

GLN

LEU

TYR

ILE

SER

VAL

CYS

SER

THR

CYS

CYS

GLN

GLU

ILE

GLY

PHE

GLN

ARG

LYS

GLN

PRO

PRO

GLY

GLU LEU

ALA LEU

ALA GLN LEU GLY

GLY LEU

GLY

GLY

GLY

LEU

GLU

VAL

ALA

GLY

ALA

LYS

ALA

PRO

ARG

ARG

GLU

ALA

GLN

ASN

ARG

GLY

TYR

THR

TYR

CYS GLY GLU

PHE

LEU VAL

VAL

ALA LEU

GLU

CYS

LEU

GLY

SER

HIS

ASN

GLN

PHE

LEU

VAL

HIS

COOH

NH -

S

S

S

S

S

S

ASN

GLN

VAL

TYR

ALA

LEU

GLU VAL

ALA

GLN

GLY

GLY LEU

GLY

GLY

GLY

LEU

PHE

LEU

Endo-activity

Exo-activity

PROTEASE FOR FOULING REDUCTION

Fouling in the evaporator leaves a

thin black substance that looks

similar to electric tape

Fouling is a large problem for the ethanol industry

Less fouling of equipment - improved downstream efficiency

Other Process Enhancement enzymes

Viscoferm®

• Used with non-rice feedstocks such as wheat, sorghum, cassava

• Reduces viscosity allowing higher DS (dry solids) content

• Improves throughput and lowers energy consumption

Provia®

• Improved yeast nutrition through better amino-acid availability

• Increased conversion efficiency of protein-bound starch

• Reduced chemical costs for nitrogen addition

Olexa ®

• Increased ethanol production due to reduction in glycerol production

• Olexa releases bound oil to significantly increase corn oil yields

Novozymes Customer – together we can…

Reduce fresh water

requirement

Reduce energy

requirement

Reduce effluent

quantity

Improve yields

Reduce Chemical

requirements

Co-product

enhancement

Improved downstream

processes

High Throughput

DELIVER MORE Services fueling success

Novozymes services: Calibrated to meet your plant’s needs

Greatest depth of process knowledge

We want to be your partner and trusted advisor with service offerings geared to give you a competitive edge and help you succeed.

Our services cover a broad range of activities:

• Data analysis and statistics

• Laboratory support

• Process optimization and troubleshooting

• Support for product trials and implementation

• Customer training

• Plant operations support

When science goes big scale

Our purpose