http://www.epa.sov/o€m/conienVn€p/products/ersof it.htmEmergency Management Last updared on ruesdav, Ausust 0e, 2011

You are here: E&LEplns Emeroencv f4anaoement NcP subpart J !ee_elgd!!!.]isIc!4cERGOFIT I4ICRO IVIX AQUA

ERGOFIT MICRO MIX AOUATECHNICAL PRODUCT BULLETINI 8.67USEPA, OIL PROGMN,I CENTERLISTING DATE: JULY 27,2011"ERGOFIT 14ICRO MIX AQUA"

I. NAIYE, BMND, OR TMDEMARK

ERGOFIT T4ICRO MIX AQUA

IISfi,glito**' Biorernediation Asent(l,licrobiological Culture, EnzynE Additlve, Nutrient

II, NAME, ADDRESS, AND TELEPHONE NUN1BER OF IVANUFACTURER/CONTACT

ER@FIT USA LLC40 E. Main Street #379Newark, DE 19711Phoner (302) 235-3085Fax: (801) 846-8043f4obile: (98s) 722-8442 or Gro) 739-9773E- rEil i iEfojOtrlCIQEdX:UEangmWeb Slte| !yldtw,mhrc0d}:!se.!gn0(lVr. Warren Russell, US Factory Representative)

III. NAN4E, ADDRESS, AND TELEPHONE NUI4BER OF PRIN4ARY DISTRIBUTORS

Svsten6 Research Intematlonal, Inc.13 Hendtage Court

Web Site : wl$u,Eisletri)c]Jsa,llE(!lr. Ram Ranbchandran, Principal Engineer)

Destrehan, LA 70047-3626Phone: (985) 764-169214obile: (985) 722-8882

IV. SPECIAL HANDLING AND WOR|<ER PRECAUTIONS FOR STORAGE AND FIELD APPUCATION

1. FlamnEbil l tvi Water-based, Non-flamnEble2. Venti lat ioni Needs no venti lat ion; aqueous-based organic product; does not emithazardous vapors. conE co2 rEy build up.3. Skin and eye contact; protective clothing; treatrnent in case of contact: ER@FIT |4ICROMIX AQUA is not a definal irritant. Avoid eye contact, and wear goggles when using product.Eyewash for quick and copious eye f lushing should be available on site. Seek pronpt rnedicalattention should contact occur and if irr i tat ion De6ists. Protective rubber oloves aresuggested during handling. ERGOFIT MICRO l'.4li AQUA has a srnell of fenne-ntation and yeast,The product does not give off harrful vapors. Long storage will cause build CO2 in watervapor4.a. lYaxiftum storage tetrperuture: 104oF4,b. l..4ininum storage tenperaturei 32oF4.c. Optinum storage temperature rangei 57oF to 97oF4.d. Ternperatures of phase separation and chenical changes: above 113oF

V. SHELF LIFE

ERmFIT MICRO l4IX AQUA has a recomnEnded shelf life of 5 years. After 5 years at

optimum storage in cool temperature thereproduct effectiveness. Shelter from directevent of fire, wash with water.

is an approxifiEte 10Vosunlight or extrenE cold

decrease per year Inor heat source. In the

1. Appllcation Method: ERGOFIT I4ICRO MIX AQUA can be applied with any conrnerciallyavailable spray apparatus, such as srnall hand held tanks, back packs, large mixing tankswith rnechanical pumping devices, vessels with boorns for spraying wide paths, or l iquid spraydevices on croD duster aimlanes or helicoDters- ERCOFIT I!4ICRO tlIX AOUA can also beapplied by eduator systenb from vessels, fire trucks, or any other outfiited vehicle.

2, Concentmtion/Application Rate: General. ER@FIT MICRO IYIX AQUA generally takes 5 to50 minutes to penetrate the nDlecular walls of hydrocarbons. Wanner conditions favor fasterbiological action whereas colder condit ions f iEy reduce the speed of deconposit ion. onceERGOFIT N4ICRO MIX AQUA is applied to the targeted areas, the nlcrobes willattachthenEelves to the hydrocarbons and start to attack their carbon structure and rEtabolizeinto natural organic substances from the hunus fanily. Aquatic plants and anlnEl life willfeed on this nutrient, Within hours odor wil l be negligent and changes wil l be noticeable inseven days, depending on the tenperature. ERGOFIT MICRO IYIX AQUA will also break downthe adhesive propert les of the hydrocaftons, preventing oi ls from attaching to aninEls,vegetation, rock5, and earth.

Dilution Rat€sr ERGOFIT IYICRO IYIX AOUA should alwavs be nixed wlth the natlve water ofthe affected area. Note: Do not use clty waterthat has been treated with chlorine withERGOFIT IYICRO l4IX AQUA, This means that if you are treatjng an affected saltwater area,then saltwater should be used as your base canler for the product. Ukewise if you aretreating a lake or river, then that carrier water would be the freshwater from that source.For general applications and light oil filr6, dilute each gallon of ERGOFIT MICRO |v X AQUAwith source water (e.q., fresh, brackish, or saltwater) at a ratio of 1:50, For oi l rpusse l ikefonrbtion ln Intert ldal zones, use a dl lut ion of 1r30. If the spll l ls very heavy and the ol l layeris thick (rDre than 1 inch) it is reconrEnded that ERGOFIT l4ICRo MtX AQUA be applied at alower ratio of 1r20, On land not Involvlng water bodies, it Is recorffnended that an organlcabsorbent be applied prior to using ERGOFIT MICRO N4IX AQUA to treat oil spills to renEdiatethe C1 and C35 Petroleum Hydrocarbons, Thls ls to increase the contact surface area forERGOFIT IYICRO lvllx AQUA to conveit spills oil and absorbent to organic hufius.

Appllcation on Coastal Wat€r: Apply the n xed solutlon usihg hand held sprayers, tanksprayers, boo116 from vessels, helicopters, or ainplahes. First, staft by spraylng the outerpedheter of the spill and systenEtically work towards the middle of the affected area,nEking sure to cover the entire surface area of the spill. Use 1.5 gallons of ERGOFIT IYICRON1IX AQUA concentrate for every 1000 gallons of hydrocarbons spilled. Dlute it with 30gallons of seawdter per gallon of concentrate for sprdying. [To calculate gallohs of ERGOFITI4ICRO llIX AQUA required, nultiply estimated gallons of hydrocarbons by 0.0015.1 Fordenser hydrocarbons, like in the case of crude oil, multiply barrels of crude oil by 42 to getgallohs of crude and apply above ratios. lf you do not know gallons of hydrocarbons orbarrels of crude oil, estirnate size of spill by (1000 square yards x 2 inch average thickness)to arrive at gallons of oil spilled. Use 10 kg/22lbs of ER@FIT I4ICRO N4IX AQUA diluted to 50gallons in water to cover this area. As a rule, 2 kgl4.4 lbs ERGOFIT MICRO l4IX AQUA isrequired per netric ton of oi l spi l led. Dilut ion varies depending on oi l thickness or sinplesheen f loating on water surface.

Application for Intertidal zones: EROFIT 14ICRO 14IX AQUA should be applied at low tide,when the affected areas are exposed to the elenEnts, allowing the product to securelyattach to the hydrocarbons, Cease application i f the t ide r ises and resume once the waterlevel has receded again. Continue application cycle unti l the entire affected area has beencoated, The recomrEnded mix ratio is 1:30. In areas where a combination of high t ide andwaves have pushed the contamination beyond the intert idalzone, oi l rd9 layer looking l ikechocolate rbusse. cordon off the area and start spravinq ERGOFIT MICRO l.4IX AOUA diluted

VI. RECOM14ENDED APPLICATION PROCEDURE

at a ratio of 1:20 in water. The objective is to get an application rdte of betwee;1 Lo 2gallons of concentrated ERGOFIT |CRO MIX AQUA sprayed per 1000 gallons of hydrocarbonssoil led,3. Conditions for Use: ERGOFIT lvlICRO llIX AQUA acts on all organic hydrocarbons on sea,fresh water, and land. As the aqe of spilled hydrocartons increases, so does the tirnenecessary for bioremediation. In general, fresh crude, gasoline of BTEX takes anywhere from30 days to 12 nbnths to conpletely biorernediate depending on weather conditions anddensity of spill. However, the rer€diation process will be noticeable throughout the process.Variations in seawater sal inity, has no effect, as long as microbial l i fe can exist, thenERGOFII N4ICRO l..4IX AQUA will be effective in salt and brackish water with a rnaximumsalinity of 4.9 percent. ER@FIT t4ICRO MIX AQUA biorernediation slows sornewhar artemperatures below 40oF. However, it will continue to work in any water temperaturcs thatwill sustaln microbial Iife. Decreasing the arnount of ER@FIT MTCRO |VIX AouA per squareyard from recomnEnded dosage rdtes lengthens the renEdiatlon tjrne. When nitrogen andcarbon is added to ERGOFIT 14ICRO MIX AQUA the inltiation of bacterial-enzynEtic activity isenhanced nEhy fold to begin bioren€diation within 2 hours of application.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:NA

b, Effectiveness:BiorenEdiation Agent Effectlveness Test (40 CFR 300.900), Federai Register September 15,19941

SunrrEry Data TablelDAYS PRODUCT TOTAL MEAN REDO/O TOTAL ItlEAN REDO/O

3 REPS/PROD ALKANES (ppm) 28 DAYS AROIVATICS (ppm) 28 DAYS

. CoNTROL 239,235 0 16,502 0" PRODUCT 239,686 0 17,431 0

, CoNTROL 217,023 9.30 L0,942 33.70' PRODUCT 17 t819 92,6 9,606 44,90

10 CoNTROL 188,379 21.3 8,989 45.50PRODUCT 2,216 99.10 3,100 82.20

Results of Gravirnetric Analysis:Percentage (o/o) Decrease in Weight of Oil on Day 28Control :1.7%Product:45,60/0

V[I. 14ICROBIOLOGICAL ANALYSIS

Microbiological Culture i1. Listing of all microorganisnE by specles and percentage in the conposition: CONFIDENTIAL2. Optimum pH, tenperature, and sElinity rdnges for use of the additive:pH: 6.5 to 7,5.I erDeft t ture: 5/ 'F to 9/-F.Salinityr The maxirrum permissible salinity is up to 4.9olo sodium bV solution3, Mininilm and rnafmum pH, tenperature, and salinity levels below or above which theeffectiveness of the additive is reduced to half its optimum capacityipH: Lower than 4.5 or higher than 8,5 reduces the product eff iciency by 50 percent.Tenperature: Lower than 32oF or higher than 104"F reduces the product efficiency by 50percent.Salinity: The nExinxlm permissible salinity is up to 4,9olo sodium by solution4. Special nutrjent reeuirernents: NA5. Test results rcgarding the determination of the presence of the following:Salrr|)nella: Neoative

Fecal coliform: NeqativeShigella: NegativeStaphylococcus Coagulase posit ive: NegativeBeta Hernclytic Streptococci: Negative

Enzyrne Additive:

1. Usting of each conponent of the total fonrulation, other than enzyrnes, by chemicalnarE and percentage by weight: CONFIDENTIAL

2. EnzynE Nan€si CONFIDENTIAL3. I.U.B.: CONFIDENTIAL4. Source of Enzymes: Flant or Fungi/Fennentation process5. unitsr No less than 1olo ahd no nbre than 50o/o bv weiqht6. Soecif ic Gravitvi 1.007. Optlrrum Cohditions:

a. pH: 7,0b. Temp€rature: 72oFc. Salinity Ranges: Fresh water to salt water up to 4.9Vod. I\4inimum and lvaxinum oHi 4.5 - 8.5e. I\4inimum and l\4axlnum iemperaturer 32oF - 104oFf. lYlnimum and l4axlrrum Sallnlty Levelsi Sallnlty levelthat will support nicrobia

actlvltv wlll not adverselv a ERGOFIT N,IICRO MIX AOUA gerfonnanceactlvlty wlll not adversely a IYICRO MIX AQUA perfonnanceEnzynt Shelf Life: ljp to 5 years when properly storedEnzynE OptlnEl Stordge Conditlons: 72oF ls optinEl, enzyrp range is freezingto 104oF, never leave ERGOFIT MICRO |v X AQUA in dlrect sunllght for rnorethan a couple of hours

Nutrient Additivei1. Listing of each cofiponent of the formulatlon by cherlcal nan€ and percentage byweight: CONFIDENTIAL2. Optlrrum storage condit ionsiTemperaturei 32oF to 104oF

PHYSICAL PROPERTIES

NA

X. ANALYSIS OF HEAVY 14ETALS. CYANIDE, AND CHLORINATED HYDROCARBONS