ERT 207-Analytical Chemistry

Lecturers:

Dr. Saleha Shamsudin (Coordinator)

Assoc. Prof. Dr. Mohamed Zulkali bin Mohamed Daud

Course synopsis: This course introduces and discusses the basic principle of analytical chemistry that covers data analysis and interpretation. Basic statistics and the utilization of statistics are applied in most of the analytical methods. In this course students are able to apply classical analytical methods such as gravimetry and titrimetry as well as applying modern methods in analytical chemistry such as chromatographic and spectroscopic technique.

Learning Outcomes:

At the end of the course, students are expected to be:

1. Ability to calculate and perform the correct statistical method for data analytical and to remember the steps in quantitative analysis.2. Ability to classify and use separation techniques and gravimetric methods for mass determination.3. Ability to differentiate and to calculate concentration of analytes of various titrimetric methods (acid-base, complexation, redox, and precipitation).4. Ability to apply the chromatography principles and to interpret and calculate peak height for concentration determination.5. Ability to understand the spectroscopic principles and to interpret and calculate concentration.

List of experiments:

1.The potentiometric titration of an acid.

2.Determination of water hardness by complexometric titration.

3.Determination of limonene concentration in citrus fruit extracts by gas chromatography.

4.Interpretation of spectra of an unknown sample using Fourier transform-infrared (FTIR) spectroscopy.

5.Determination of active drug substance in a pharmaceutical formulation using ultraviolet and visible spectrometry (uv/vis).

Learning approach: Lecture : 42 hours (60 %)Laboratory : 20 hours (28.6 %)Tutorial : 8 hours (11.4 %)

Learning approach: Lecture : 42 hours (60 %)Laboratory : 20 hours (28.6 %)Tutorial : 8 hours (11.4 %)

Evaluation contribution: •Peperiksaan / Examination : 70 %•Mid Term Examination 1 = 10 %•Mid Term Examination 2 = 10 %•Final Examination = 50 %

•Penilaian berterusan / Continual assessment : 30 %•Laboratories = 15 %Assignments / Quizzes = 15 %

Evaluation contribution: •Peperiksaan / Examination : 70 %•Mid Term Examination 1 = 10 %•Mid Term Examination 2 = 10 %•Final Examination = 50 %

•Penilaian berterusan / Continual assessment : 30 %•Laboratories = 15 %Assignments / Quizzes = 15 %

List of text books and references :

Buku Teks/ Text book(s):•Gary D. Christian (2004). Analytical Chemistry. 6th Edition. Publisher: John Wiley & Sons, Inc.

Buku Rujukan/ Reference books:•David Harvey (2000). Modern Analytical Chemistry. Publisher: McGraw-Hill•D. Keely and P. J. Haines (2002). Analytical Chemistry. Publisher: Oxford: Bios ScientificD. A. Skoog, D. M. West and F. J. Holler (1996). Fundamentals of Analytical Chemistry. Publisher: Saunders College Publication.

Minggu/Week

Kandungan Kursus / Course Contents(Panduan/Guidelines)

Tugasan Bacaan/Reading

Assignment

Pensyarah/

Lecturer

Week 1(9/09 – 13/09)

INTRODUCTION TO ANALYTICAL CHEMISTRY

Explain qualitative and quantitative. Repeat the steps in quantitative analysis or unit operation in analytical chemistry.

Chapter 1Dr.

Saleha

Week 2 – 3(16/09 – 27/09)

BASIC STATISTICS

Define accuracy and precision, remember ways of describing accuracy and precision, types of errors, understand the concept of significant figures, standard deviation.

UTILIZATION OF STATISTICS IN DATA ANALYSIS

Identify the significant testing. Calculate the T test and Q test.

CALIBRATION CURVE

Interpret slope, intercept, coefficient correlation and coefficient of determination (r2)

Chapter 1Dr.

Saleha

Week 4 - 5(30/9 – 11/10)

CHEMICAL EQUILIBRIUM

Discuss the concept of chemical reaction: the rate concept, type of equilibria, Le Chatelier’s principle. Effects of temperature, pressure and concentration on equilibria.

ACID-BASE EQUILIBRIUM

Repeat acids and bases theories, acid base equilibria in water, pH scale. Discuss weak acids and bases, calculate the pH of salt of weak acids and bases. Discuss buffers. Introduce strong acids and strong bases, Apply detection of end point, indicators. Discuss weak base versus strong acid.

Chapter 2Dr.

Saleha

Week 6(14/10 - 18/10)

Cuti Pertengahan Semester / Mid-semester Break

Week 7 - 8(21/10 –

1/11)

PREPARING SAMPLES FOR ANALYSIS

Discuss type of separation techniques. Classify separation techniques based on chemical or physical properties. Classify separation based on a partitioning between phases. Calculate partition coefficients and distribution ratios.

Chapter 3Dr.

Saleha

Week 9-10 (4/11 – 15/11)

CLASSICAL METHODS IN TECHNIQUES OF ANALYTICAL CHEMISTRY: TITRIMETRIC METHODS OF ANALYSIS

Explain acid-base, redox, complexation and precipitation titrimetry. Discuss on the reactions involved. Calculate the quantitative and qualitative application of acid-base, redox, complexation, precipitation titrimetry. List other application titrimetry.

Chapter 4

Assoc. Prof Dr.

Mohamed Zulkali bin Mohamed

Daud

Week 11 – 13

(18/11 – 29/11)

MODERN METHODS IN TECHNIQUES OF ANALYTICAL CHEMISTRY: CHROMATOGRAPHIC AND ELECTROPHORETIC METHODS

Introduce principles of chromatography and electrophoresis. Discuss gas chromatography and applications. Calculate quantitative and qualitative applications of gas chromatography (GC). Discuss high-performance liquid chromatography (HPLC) and applications. Calculate quantitative and qualitative applications of high-performance liquid chromatography (HPLC). Discuss electrophoresis and its applications in protein and DNA analysis.

Chapter 5

Assoc. Prof Dr.

Mohamed Zulkali bin Mohamed

Daud

Week 14 - 15

(2/12 – 13/12)

MODERN METHODS IN TECHNIQUES OF ANALYTICAL CHEMISTRY: SPECTROSCOPIC TECHNIQUES

Explain the properties of electromagnetic radiation and relate with spectroscopic techniques. Introduce techniques on spectroscopy based on absorption (ultraviolet-visible and Fourier transform-infrared spectroscopy (FT-IR) and atomic absorption spectroscopy (AAS). Demonstrate qualitative and quantitative applications of UV/visible, infrared and atomic absorption spectroscopic techniques in biological analysis. Calculate concentration using spectroscopic techniques.

Chapter 6

Assoc. Prof Dr. Mohamed Zulkali

bin Mohamed Daud

Week 16(16/12– 20/01)

Minggu Ulangkaji / Revision Weeks

Week 17 - 20

(23/12 – 10/01)

Peperiksaan Akhir Semester/ Final Examination

LABORATORY PLAN

GroupA1, A2 & A3 A4, A5 & A6 B1, B2 & B3 B4, B5 & B6

1 Introduction to Analytical Chemistry

2 Hari Malaysia

3 Exp 1 Exp 5 Tutorial 1

4 Tutorial 1 Exp 1 Exp 5

5Exp 5 Exp 1 Tutorial 2

6Mid Semester Break

7 Tutorial 2 Exp 5 Exp 1

8Exp 4 Tutorial 3

9Tutorial 3 Exp 4

10Exp 2 Exp 3 Tutorial 4

11Tutorial 4 Exp 2 Exp 3

12Exp 3 Exp 2 Tutorial 5

13 Tutorial 5 Exp 3 Exp 2

14 Tutorial 6

15Lab Test

16Study Week

A1 B1AINA MADIA BINTI RAZALINUR HAZIRAH BINTI MOHD YUSOFFSITI NURSHAMILA BINTI ZAMZURYMOHAMAD AMIN BIN AWANG TEH @ ISMAILLIM SHIN YUANANG LI WEI

CHOO YEE CHINGNUR AMALINA BINTI ABDULLAH SANISITI KHADIJAH BINTI MASOR @ IBRAHIMKWAN SIN YENASSADUL HAQ ALI HAMIDIBOO CHIE HANG

A2 B2MUHAMMAD FAIZ BIN ZAINOL BAHRINUR AMIRAH BINTI MAHFODSAW WAN LINGNUR ASYILAH AKMAL BINTI ABDULLAHMOH LAI KUANANG KIAN THING

NORHIDAYAH BINTI MOHAMADNORHAIZA NADIA BINTI HALIB @ YAHAYAONG LI JINGMOHD SHAFIQ BIN MD ZINANNIEBELL A/P STANLEY LOURDES L.BENEDICTJASMINE FONG JIE MIN

A3 B3NURUL NABILLATUL MUNIFA BINTI R. AZMIPUA CHEE WEEPUTRI NOOR NATASHA BINTI CHE YEO MEGATNADYA ORSELA ANDICOCHUAH SIN YEADZDZIN SHAFWAN BIN ADLAN

SITI NABIHAH BINTI AB MAJIDTAN HUAN YIHRAYMOND LIM HUNG TATNUR ATHIRAH AWATIF BINTI ABDUL RAHMANLAU KIA KIANCHONG WAIN KET

LAB GROUPS

A4 B4

YUHANEES SYUHADAH BINTI MALIHANNUR AMALINA BINTI MUHAMAD ZAIDINUR AMIRAH ASIFA RAISHA BINTI ZAHARINABILAH BINTI HUSAINMOHAMAD ADIB BIN EDRISCHEE CHEAU YEE

WONG JING RUINURUL NADIAH BINTI MOHAMAD NIZAMNUR SYAZWANI BINTI ISMAILMUHAMMAD SYAFIQ BIN SANILEONG YONG YOUCHEONG XIAN ZHI

A5 B5

VIVIAN LEE JIAQIANONG YU LIANNURUL SHAKIRA BINTI JOHARINOR SYAHIDA BINTI ISMAILGOOI MING YANSHARIFAH SABIRAH BINTI SYED HAMZAH

TENGKU INTAN NADZRAHSITI AISYAH BINTI MOHD RAMLINUR HIDAYU BINTI MISRANNG ZHANG JINMUHAMAD IKRAM BIN JAMALUDINAMIRA NASYRAH BINTI MOHD HUSIN

A6 B6

SITI RABAAH BINTI HAMZAHTAN SIEW YENNURAINI BINTI ABDULLAHNABILAH AFIQAH BINTI SUHAIMIMUN HUI SIN

ONG XIN SHINURULFARAHANA BINTI HARUNNOR ATIQAH BINTI JUSRILNG PEY XUANANIS USMANI

Important Note: Your attendance to lecture / tutorial / laboratory is compulsory. If absent without reason, the student can not sit for the final examination.