estimation of phosphate07_27_0… · how can phosphorus be distributed throughout the body?...
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Blood Phosphorus
Presented By
Assist.Lecturer Aseel Ghassan Daoud
M.Sc. In pharmacy/ Clinical laboratory sciences
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What is phosphorus?
• It is a mineral that combines with other substances
to form organic and inorganic phosphate
compounds.
What are functions of phosphates?
Energy production
Muscle and nerve function
Bone growth
Buffer that maintains acid-base balance
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What are phosphorus rich foods?
Egg
Dairy products
Cereals
Beef
Chicken
Nuts
Fish
Beans
Peas
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How can phosphorus be distributed throughout the
body?
• About 70%-80% of the body’s phosphates are
combined with calcium to help form bones and
teeth.
• About 10% are found in muscles
• About 1% in nerve tissue
• Only 1% found in blood
• The rest are distributed within cells throughout
the body to store energy.
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How can the body regulate blood phosphate
level ?
How much it absorbs from the intestines
How much it excretes via kidneys
It is also affected by parathyroid hormone (PTH),
calcium and vitamin D.
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What are symptoms of low phosphate levels?
Fatigue
Muscle weakness
Cramps
Bone problems
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Causes of hypophosphatemia:
Hypercalcaemia and high level of PTH
Overuse of diuretics
Malnutrition
Diabetic ketoacidosis (after treatment)
Hypothyroidism
Hypokalemia
Rickets due to vit. D deficiency
Sever burns
Alcoholism
Chronic antacid use
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Causes of hyperphosphatemia:
Kidney failure
Hypoparathyroidism
Hypocalcaemia
Diabetic ketoacidosis (first seen)
Phosphate supplementation
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High phosphorus level can lead to organ
damage, why?
due to calcification, deposits of calcium
phosphate in tissues.
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Reference level:
• 12-60 years : 2.7-4.5 mg/dl
Why are phosphate levels in children
higher than in adults?
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SpectrophotometerMicropipette
Phosphorus kit
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Disposable Tips
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CuvettesTest tube
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Working Procedure:
1. Bring reagents and samples to room temperature.
2. Pipette into labeled test tubes
Tubes Blank Sample Standard
Working reagent
R1
1 ml 1 ml 1 ml
Sample - 50 μ -
Standard - - 50 μ
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3. Mix, let stand the tubes for 1 min. then pipette:
4. Mix, and let stand the tubes for 10 min. at room
temperature
5. Read the absorbance (A) of the sample and
standard at 740 nm against the reagent blank.
Developer
R2
0.5 ml 0.5 ml 0.5 ml
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Calculations:
Serum :
A (sample) * concentration of standard (known) mg/dL= mg/dL phosphorus
A (standard)
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