eu- interreg daimon wp 2.5 mechanistic approach to the ... fileproducts: fluorometri and...
TRANSCRIPT
EU- Interreg Daimon WP 2.5 Mechanistic approach to the toxicity of marine warfare products: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological Chemistry, Alfred-Wegener-Institut Helmholtz Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, D-27570 Bremerhaven
SETAC Europe 24th Annual Meeting - 2014
Biomarker for mussel tissue analysis:
Gills
Oxidative stress
Digestive gland
Oxidative stress
General stress
Haemocytes
Cytotoxic effects
Immunotoxicity
Cellular transport mechanisms: MDR, MRP
etc.
Rivera-Ingraham et al. 2016 Front Zool
Rivera-Ingraham et al. 2016 Front Zool
Tietje et al 2013 Mar Drugs
Measurements with Fura-II AM
R= F (340/380)
[Ca+
+ i]
K+
Ca 2+
A B
140
120
100
80
60
40
20
0
I/I m
ax [%
]
-80 -60 -40 -20 0 20 40 60
U [mV]
1200
1000
800
600
400
200
0
I [p
A]
200150100500
time [ms]
+20 mV -70 mV
+40 mV +60 mV
A
100
80
60
40
20
0
I/I m
ax [%
]
-80 -60 -40 -20 0 20 40 60
U [mV]
-50
0
50
I [p
A]
200150100500
time [ms]
+20 mV
-70 mV
50 ms
50 pA
120
100
80
60
40
20
0
I/I m
ax [%
]
0.01 0.1 1 10 100 1000
2,4,6-tribromophenol [µM]
B
Thank you for your attention
2,4,6-Trinitrotoluol
2-Amino-4,6,dinitrotoluol
4-Amnino, 2,6-Dinitrotoluol
0min 2 min 4 min
6 min 8 min 10 min
2.5
2.0
1.5
1.0
0.5
0 2 4 6 8 10
Time (min)
JC-1
0 a
ggre
gate
: mo
no
me
r
***
JC-1
0 a
ggre
gate
: mo
no
me
r 3
2
1
0
5’ FCCP control
A1
B1
B2
5’ F
CC
Pco
ntr
ol
A2
+ ADP 5 mM