eu- interreg daimon wp 2.5 mechanistic approach to the ... fileproducts: fluorometri and...

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EU- Interreg Daimon WP 2.5 Mechanistic approach to the toxicity of marine warfare products: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological Chemistry, Alfred-Wegener-Institut Helmholtz Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, D-27570 Bremerhaven

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Page 1: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

EU- Interreg Daimon WP 2.5 Mechanistic approach to the toxicity of marine warfare products: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological Chemistry, Alfred-Wegener-Institut Helmholtz Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, D-27570 Bremerhaven

Page 2: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological
Page 3: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

SETAC Europe 24th Annual Meeting - 2014

Biomarker for mussel tissue analysis:

Gills

Oxidative stress

Digestive gland

Oxidative stress

General stress

Haemocytes

Cytotoxic effects

Immunotoxicity

Cellular transport mechanisms: MDR, MRP

etc.

Page 4: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

Rivera-Ingraham et al. 2016 Front Zool

Page 5: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

Rivera-Ingraham et al. 2016 Front Zool

Page 6: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

Tietje et al 2013 Mar Drugs

Page 7: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological
Page 8: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

Measurements with Fura-II AM

R= F (340/380)

Page 9: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

[Ca+

+ i]

Page 10: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

K+

Ca 2+

Page 11: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological
Page 12: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological
Page 13: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

A B

140

120

100

80

60

40

20

0

I/I m

ax [%

]

-80 -60 -40 -20 0 20 40 60

U [mV]

1200

1000

800

600

400

200

0

I [p

A]

200150100500

time [ms]

+20 mV -70 mV

+40 mV +60 mV

Page 14: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

A

100

80

60

40

20

0

I/I m

ax [%

]

-80 -60 -40 -20 0 20 40 60

U [mV]

-50

0

50

I [p

A]

200150100500

time [ms]

+20 mV

-70 mV

50 ms

50 pA

120

100

80

60

40

20

0

I/I m

ax [%

]

0.01 0.1 1 10 100 1000

2,4,6-tribromophenol [µM]

B

Page 15: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

Thank you for your attention

Page 16: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological
Page 17: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

2,4,6-Trinitrotoluol

2-Amino-4,6,dinitrotoluol

4-Amnino, 2,6-Dinitrotoluol

Page 18: EU- Interreg Daimon WP 2.5 Mechanistic approach to the ... fileproducts: Fluorometri and Electrophysiology of physiological parameter Matthias Brenner and Ulf Bickmeyer Section Ecological

0min 2 min 4 min

6 min 8 min 10 min

2.5

2.0

1.5

1.0

0.5

0 2 4 6 8 10

Time (min)

JC-1

0 a

ggre

gate

: mo

no

me

r

***

JC-1

0 a

ggre

gate

: mo

no

me

r 3

2

1

0

5’ FCCP control

A1

B1

B2

5’ F

CC

Pco

ntr

ol

A2

+ ADP 5 mM