eukaryotes and viruses - doc...
TRANSCRIPT
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Eukaryotes and Viruses
Chapters 12 and 13
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Fungi
Heterotrophic, Mainly Opportunistic Pathogens
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Distinguishing Characteristics of
Fungi
• Chemoheterotrophic
• Cells walls composed of Chitin
• Diverse Metabolic Capabilities for Complex
Carbohydrates
• Xerophilic
• Aerobic/Facultative Anaerobes
• Prefer Low pH
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Vegetative Growth
• Filamentous Fungi
• Yeasts
• Dimorphic Fungi
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Filamentous Fungi
• Hyphae (individual
strands)
– Septate
– Coenocytic
• Mycelium (mass of
hyphae)
• Aerial Hyphae
• Mass of Conidia
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Yeast
• Bud
• Bud Scar
• Pseudohyphae
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Dimorphic Fungi
• Medically very
important
• Hyphae in the
Environment, Yeast
in the host
• Temperature and
CO2 are common
triggers
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Fungal Lifecycle
• Haplodiplontic Lifecycles
• Asexual Cycle
• Sexual Cycle
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Haplodiplontic Life Cycle
Gametophyte (n)
Sporophyte (2n)
Egg
Sperm
Zygote
Embryo
Sporangia
Sporocyte
Spores
Haploid
Diploid
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Asexual Spores
• Genetically Identical to the parent
• Genetically Haploid
• Several Types
– Conidia
– Blastoconidia
– Arthroconidia
– Chlamydoconidia
– Sporangiospores
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Sexual Spores
• Haploid Spores Arising
from a Diploid Cell
• Genetic Recombination
of compatible mating
types
• Fungi are classified on
the basis of their sexual
cycles.
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Medically Important Phyla
• Zygomycota
• Ascomycota
• Basidiomycota
• Deuteromycota (Asexual Fungi)
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Zygomycota
• Coenocytic Hyphae
• Not a phylogentically
distinct group.
• Sporoangiospores and
Zygospores
• Rhizopus is a common
genus.
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Ascomycota
• Septate Hyphae and Yeasts
• Largest group of classified fungi
• Most Deuteromycota are classified in this group by Genetics
• Ascospores (in an ascus) and Conidia
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Basidiomycota
• Septate Hyphae
• Basidiospores
produced on Basidium,
some produce conidia
(though this is crude
terminology)
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Deuteromycota
• Depreciated, though still used Taxon
• Holding Phyla with no observed sexual state
• Most have been reclassified as Ascomycota
based on Genetics
• Leads to confusion over nomenclature
– Telomorph : Sexual State (preferred name)
– Anamorph : Asexual State (common name)
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Fungal Disease
• Mycoses are not common but difficult to
treat.
• Mycoses are defined by the depth of tissue
affected.
• Most fungi are either superficial or
opportunistic pathogens… though overt
pathogens exist.
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Examples of Fungal Disease
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Protozoa
Diverse Unicellular Eukarya Pathogens
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Protozoans
• Phylogenetically, a diverse and ill-defined
group.
• Medically we are worried about the
heterotrophs, not the photosynthetic phyla
• Complex lifecycles with unique stages
– Trophozoite
– Schizogony
– Cyst
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Protists Are NOT Monophyletic
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Archaezoa
• Lack Mitochondria,
but possess relics
called mitosomes.
• Move by means of
Flagella
• Possess two nuclei.
Giardia intestinalis
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Microspora
• No mitochondria
• No microtubules
• Obligate intra-
cellular pathogens
• Common in AIDS
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Amoebozoa
• Phylogenetically
these organisms are
not linked to a
definite clade.
• Movement through
pseudopods
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Apicomplexa
• Named for the
Apical complex, an
organelle used for
cell penetration.
• Complex Lifecycles
with both a
definitive and
intermediate host
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Plasmodium species
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Eugelnozoa
• Hemoflagellates, more appropriately called
Kinetoplastids, are the pathogenic
members.
• Possess unique single mitochondrion called
kinetoplasts.
• Many are Parasitic
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Helminths
The Worms
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Characteristics of Pathogens
• They may lack a digestive system
• They have a reduced nervous system
• Lacking or atrophied movement systems
• Complex reproductive systems
• May be dioecious or monoecious
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Platyhelminthes
• Flatworms, so called for overall flat body
plan.
• Actually the Subphylum Neodermata
• All have a Neodermis (also called a cuticle)
to protect them from the host and lack
adaptations such as eyepores (found in
free-living flatworms)
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Trematodes
• Flukes
• Ventral and Oral
Sucker to attach to
host tissue.
• Life Cycles involve
more than a single
host and mutiple
developmental stages
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Schistosoma Life Cycle
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Cestodes
• Tapeworms
• Three body sections,
scolex, neck and
proglottids
• No digestive system
• Mature proglottids
are released through
feces of host.
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Phylum Nematoda
• Roundworms, due to the circular body
cross-section.
• Not to be confused with Phylum Annileda,
the segmented worms (i.e. Earthworms)
• Complete digestive systems
• Sexually dimorphic
• Numerous through out the environment
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Comparative Anatomy
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Nematoda Diseases
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Viral Characteristics and Structure
Why Viruses aren’t Alive
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General Characteristics of Viruses
• Name derives from the Latin for “poison”
• Obligatory intracellular parasites
• Referred to as filterable
• Contain a single type of nucleic material
• The nucleic material is covered in a protein
coat.
• Use the synthesis machinery of the host to
multiply.
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Why are they not Alive?
• No independent metabolism or
reproduction
• No single phylogenetic origin
• No cellular structure
• No ribosomes
• Though they DO evolve and reproduce.
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Host Range
• Viruses have a specific subset of cell types
they will infect, referred to as Host Range.
• Most viruses can only infect a single species
• Some viruses can cross species barriers
• Numerous factors influence host range
• Viruses that infect bacteria are referred to
as bacteriophage or simply phage.
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Viral Particle Size
Staphylococcus Bacteria 1 μm in diameter
Poxviridae
Lentiviruses
Picornaviridae
Bacteriophage
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Viral Structure
• A Virion is a complete, infectious viral
particle and is composed of…
– Nucleic Acid
– Capsid and Envelope
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Nucleic Acid
• Only a single type of nucleic acid (RNA or
DNA) is present in any species of virus.
• Unlike cellular life, viral nucleic acid can be
either single or double-stranded (again only
a single type per species)
• Size of the genetic structure can range from
a few thousand base pairs to a quarter of a
million
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Capsid and Envelope
• Capsids are regular repeating protein
structures composed of capsomeres.
• Some viral species also have a host-derived
envelope surrounding the capsid
• Some viral species have protein/
carbohydrate “spikes” rising from the
surface that can be used for identification
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Viral Morphology
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Viral Taxonomy
How do you do a taxonomy of something that isn’t alive?
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Viral Taxonomy
• Without a shared phylogeny there is no use for the higher taxons (Domain, Kingdom, Phylum, and Class)
• Typically viral species are referred to by Order, Family, Genus and a descriptive common name (in place of a species epithet)
• Based on – Nucleic Acid type
– Strategy of replication
– Morphology
– Host range
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Viral Replication
No, not 1 becomes 2, more like 1 becomes 1000.
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Growing Bacteriophage
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Growing Animal Viruses
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Viral Identification
• Polyphasic Identification
– Morphology
– Detection of Antibodies
– Western Blotting of known viral proteins
– Nucleic Methodologies
• PCR
• RFLP
• RNA PCR
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Lytic Bacteriophage Cycle
Attachment
Penetration
Biosynthesis Maturation
Release
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Lysogenic Bacteriophage Cycle
Lytic Cycle
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Animal Unenveloped DNA Viral
Replication
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DNA vs. RNA Viral Replication
• There are various types of RNA viruses.
• Replication of the Genetic Material can be simple or a multistep process. – +RNA, direct translation and replication by viral
protein
– -RNA, indirect translation and replication by viral protein
– dsRNA, direct translation and relication by viral protein
– Retroviruses, conversion of RNA to DNA, integration and then production by host.
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Comparison
Bacteriophage
• Attachment to Cell Wall
proteins.
• Viral DNA is injected into
cell
• No removal of capsid
required
• Biosynthesis in cytoplasm
• Lysogeny
• Host cell lysed for release
Animal
• Attachment to plasma
membrane proteins and
glycoproteins.
• Capsid enters cells
• Capsid removed by enzymes
• Biosynthesis in nucleus or
cytoplasm
• Latency
• Enveloped viruses bud and
nonenveloped rupture.
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Viruses and Cancer
One of many factors
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Oncogenic Viruses
• Some viruses and known to help trigger cancers, called oncogenic viruses.
• These viruses affect oncogenes, natural parts of our genetic structure that can cause cancer.
• The process of becoming cancerous is termed transformation.
• Oncogenic Viruses integrate into the host genetic material.
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Known Oncogenic Viruses
Type of Virus Viral Species Associated Cancer
DNA Human Papillomavirus Cervical Cancer
Epstein-Barr Virus Burkitt’s Lymphoma
Hepatitis B Virus Liver Cancer
Kaposi Sarcoma-Associated Hepervirus
Kaposi Sarcoma
Merkel Cell Polyomavirus Merkel Cell Carcinoma
RNA Human T-Lymphotropic Virus 1 Leukemia
Hepatitis C Virus Liver Cancer
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Latency, Persistence and the
Prions
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Acute, Latent, and Persistent Viral
Infections
• Acute Infections are those that cause
immediate proliferation.
• Latent infections can occur by itself or after
an acute infection, where the viral load
remains undetected for a long period of
time before reemerging quickly.
• Persistent Infections are ones where the
viral load build over a long period of time.
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Graph of Acute, Latent and
Persistent Viral Infections
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Prions
• Prions are infectious protein particles
• Prions are altered forms of a normal protein in the host that can catalyze the alteration of other “normal” protein particles to the “prion” state
• They cause neurological degradation and death with no known treatment.
• Since each prion protein is infectious, they are extremely resistant to control measures.
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Prion Reaction
PrPC + PrPSc 2 PrPSc