european congress on biotechnology ; 5 …modelling and simulation of hyphal growth, metabolism and...
TRANSCRIPT
5th European Congress on
BiotechnologyCopenhagen, July 8-13,1990
Proceedings vol. 1
Edited by
Claus Christiansen
DSc, MD, Managing Director, The Genetic Engineering Group, Lyngby
Lars Munck
Fil.Dr., Research Manager, Carlsberg Research Laboratory, Copenhagen
John Villadsen
Dr.techn., Professor, DTH, Lyngby
UNIVERSEATSDSUOTHEkHANNOVER
TcOHN!SG44E
1 INFORMATIONSBIBUOTHEK
Munksgaard
VOLUME I
GENERAL LECTURES
Genetics and gene ethics.
Philipson, L p. 3
Protein engineering and insulin.
odson, GG, Derewenda, U p. 11
Microbial physiology and rec-DNA technology "The microbe,more than a bag of enzymes".
Harder, W, Dijkhuizen, L p. 23
The enzyme industry and modern biotechnology.Riisgaard, S p. 31
The evolution of downstream processing.Van
't Riet, K p. 41
Modern biotechnology and the food industry.
Allen, G p. 50
DNA fingerprinting; Science, applications and impact.Debenham, PG p. 60
Biotechnology research for developing countries.
Cocking, EC p. 67
How can biotechnology solve environmental problems of
modern society?Verstraete, W, Huysman, F, Top, E p. 75
The structure, function and pathology of the human
mitochondrial genome.
Lestienne, P, N0rby, S p. 85
SYMPOSIA
Exportable biotechnological products from developing countries
Exportable biotechnological products from developingcountries.
Rolz, CE p. 97
Delicate mushrooms for the export markets in indus¬
trialized countries.
Chang, ST p. 100
Considerations in the development of exportable bio¬
technological products in developing countries.
Mou, D-G p. 104
Improved plant processing by simultaneous lactic acid
fermentation and enzymatic hydrolysis.
Tengerdy, RP P•108
Plant cell culture - industrial applications
Plant cell culture - a technology whose time has come?
Fowler, MW P- H2
The selectivity in the biotransformation of testoste-
rones with the cultured cells of Marchantia polymorpha.
Hamada, H, Kawabe, S P- 120
Effect of in situ extraction and cell immobilization of
L. erythrorhizon on shikonin production.
Kim, DJ, Chang, HN p. 124
Effect of mixed nitrogen sources on the growth of plant
cells in continuous culture.
van Gulik, WM, ten Hoopen, HJG, Heijnen, JJ, Libbenga, KR,
Luyben, KChAM p. 128
The effects of precursor feeding on alkaloid production
in Coffea arabica plant cell cultures in a novel membrane
reactor.
Lang, JA, Yoon, K-H, Prenosil, JE p. 132
Microbial plant protection agents
Microbial crop protection and enhancement agents: Old
problems, new perspectives.
Lynch, JM p. 136
Continuous processing of Bacillus thuringiensis.
Dreier, P, Brauhart, I, Rung, W, Moser, A p. 142
Scale-up of beta exotoxin production in fed-batch Ba¬
cillus thuringiensis fermentations.
Paige, MR, Cooper, RD p. 146
Production of siderophores in antagonistic and non-
antagonistic strains of Pseudomonas.
Jacques, Ph, Ogena, M, Klinkenberg, H, Lepoivre, Ph,Thonart, Ph, Delfosse, Ph p. 150
Plant protection and growth promotion by Trichoderma
harzianum mutants.
Tengerdy, RP, Baker, R, Sary, L p. 153
Monitoring and control in environmental biotechnology
Multi-analyte enzyme electrodes for environmental moni¬
toring.Henkens, RW, O'Daly, JP, Zhao, J p. 157
Rodtox: Biosensor for rapid determination of the bio¬
chemical oxygen demand and the on-line monitoring of
the toxicity of wastewaters.
Vanrolleghem, P, Dries, D, Verstraete, W p. 161
Environmental microbial and biochemical monitoring of
biotechnological processes.Bennett, AM, Hill, SE, Benbough, JE, Hambleton, P p. 165
The construction of broad host range genetic markers
and their use in monitoring the release of catabolic
gems to aquatic environments.
McClure, NC, Saint, CP, Fry, JC, Weightman, AJ p. 169
Design of reduced order adaptive controllers for
anaerobic digestion processes.
Bastin, G, van Breusegem, V, Dochain, D, Nyns, E-J,Renard, P p. 173
Vaccine production by genetic engineering
A new vaccine against whooping cough containing gene¬
tically detoxified pertussis toxin.
Rappouli, R, Pizza, M, Covacci, A, Bartoloni, A, Perugini,M, Manetti, R, Bugnoli, M, Ruggiero, P, Olivieri, R,De Magistris, T, Nencioni, L p. 177
Rationally attenuated Salmonella strains as live oral
vaccines.
Chatfield, SN, Fairweather, N, Roberts, M, Tite, J,
Charles, I, Dougan, G p. 182
An approach to the development of a malaria vaccine.
Borre, M, Dziegiel, M, Petersen, E, H0gh, B, Jepsen, S,
Vuust, J, Rieneck, K, Riley, E p. 186
Development of a recombinant vaccine against progres¬
sive atrophic rhinitis in pigs.
Petersen, SK, Bording, A, Foged, NT, Frandsen, PL,
Riemann, HK, Nielsen, JP, S0rensen, V p. 190
Production of meningococcal class I outer membrane
protein in Bacillus subtilis.
Chattopadhyay, P, Butcher, S, Nurminen, M, Wahlstrohm, E,
Sarvas, M, Runeberg-Nyman, K, Puohiniemi, R p. 194
Expression of heterologous proteins in fungi
Introduction to production of heterologous proteinsin fungi.Ward, M p. 198
Trichoderma: A fungal expression system for the pro¬
duction of heterologous proteins.Penttila, M, Uusitalo, J, Hirvonen, A, Nyyssonen, E,
Saloheimo, M, Keranen, S, Knowles, J p. 200
Secretion of active calf chymosin from Mucor circinel-
loides.
Str0man, P, Bruyant, P, Jepsen, LP, Wind, A, Arnau, J ... p. 203
An inducible gene expression system for Neurospora crassa.
Collins, ME, Briggs, G, Sawyer, C, Sheffield, PJ, Con-
nerton, IF, Gainey, LDS p. 207
The commercial development of heterologous gene ex¬
pression in Aspergillus.Ward, M p. 211
Efficient/intelligent screening for microbial potential
Efficient/intelligent screening for microbial potentials.de Bont, JAM p. 215
Microbial emulsification of crude oil.
Krigsvoll, S, Josefsen, K, Storr0, I p. 221
'In vivo protein engineering': A goal-orientated approachfor biocatalyst screening and optimization.
Hermes, HFM, Peeters, PJH, Peeters, WPH, Schepers, CHM,
Kamphuis, J, Schoemaker, HE, Meijer, EM p. 225
Potential of an archaebacterial system for screening DNA
binding drugs and inhibitors of type II DNA topoisomerases.Gadelle, D, Forterre, P p. 229
New applications of industrial enzymes
New developments in the application of enzyme catalyststo synthesis reactions.
Monsan, P, Paul, F p. 233
Enzymatic reactions in supercritical carbon dioxide
and integrated product-recovery.
Doddema, HJ, Janssens, RJJ, de Jong, JPJ, van der Lugt,
JP, Oostrom, HHM p. 239
Extractive biocatalysis in a liquid-impelled loop reactor.
Vermue, MH, Tramper, J p. 243
Synthesis of lambda-leucovorin by the combination of
chemical and enzymatic processes.
Uwajima, T, Eguchi, T, Oshiro, T, Kuge, Y, Igarashi, A,
Horiguchi, A, Mochida, K, Iwakura, M p. 247
L(-)-carnitine synthesis by stereoselective hydrationof crotonobetaine.
Jung, H, Kleber, H-P p. 251
Metabolic pathway engineering for the production of
new chemicals.
Rozzell, JD, Voloch, M, Lynch, J, Maxwell, P p. 255
Modelling of fermentation kinetics
Modelling of fermentation kinetics.
Villadsen, J, Nielsen, J p. 259
A population balance model of enzymatic lysis of mi¬
crobial cells.
Asenjo, JA, Hunter, JB p. 267
Application of dynamic programming for fermentative
ethanol production by Zymomonas mobilis.
Rivera, SL, Karim, MN p. 271
Modelling of the production on complex media of peni¬cillin acylase by a recombinant E. coli.
Cayuela, C, Taha, S, Boudrant, J, Engasser, J-M p. 275
Modelling and simulation of hyphal growth, metabolism
and mass transfer in pellets of Streptomyces.Buschulte, TK, Gilles, ED p. 279
Lactic acid bacteria in the food industry
Lactic acid bacteria in the food industry: State of
the art.
Mogensen, G p. 283
Analysis of secretion signals of lactococci.
Simons, G, van Asseldonk, M, Rutten, G, Nijhuis, M,
Homes, M, de Vos, WM p. 290
Analysis of strain diversity among Lactococcus lactis
strains in commercial use.
Horgan, J, Aalvik, C, Outzen, R, Hansen, E, Myling-Petersen, D, Larsen, I p. 294
Effect of oxygen on growth and lactose metabolism of
Lactobacillus fermentum.
Vahvaselka, M, Hofman, M, Linko, P p. 298
Production of antimicrobial compounds by lactic acid
bacteria.
Barnby-Smith, FM, Roller, SD p. 302
Lactococcus lactis 10-1 (JCM 7638), a new strain of
homofermentative L-lactate producing coccus.
Ishizaki, A, Hara, T, Ezaki, T p. 306
Safety in biotechnology - social, scientific and legal aspects
The "Good industrial large scale practice" (GILSP)
concept of the organisation for economic co-operationand development (OECD).
Ktienzi, MT p. 310
The USDA experience in moving agricultural biotechnologyinto the field.
Young, AL, Steinbock, MB P- 315
Occupational safety and health management in some bio¬
technological companies in Denmark, Sweden, Netherlands,England and USA.
Boyd, HB, Broberg, 0, Mikkelsen, SH p. 319
Biotechnology of anaerobes
Biotechnology of anaerobes.
Cole, JP- 323
Genetics of denitrification by Alcaligenes eutrophus.Friedrich, B, Warnecke, U, Sann, R, Schneider, B p. 327
Acetate activation in Methanothrix soehngenii.Jetten, MSM, Stams, AJM, Zehnder, AJB p. 331
Improved growth and methane production conditions for
Methanobacterium thermoautotrophicum.Gerhard, E, Butsch, BM, Marison, I, von Stockar, U p. 336
Mixed substrate feeding strategies to improve the
biotechnological potential of strict anaerobes.
Lindley, ND, Loubiere, P, Vasconcelos, I, Soucaille, P,Gros, E
p. 339
Anaerobic, thermophilic, cellulose and hemicellulose
degrading bacteria.
Mathrani, IM, Ahring, BKp. 343
Effects of lipids on thermophilic anaerobic digestion andreduction of lipid inhibition upon addition of bentonite.Ahring, BK, Angelidaki, I
p. 347
Preliminary laboratory result on the use of an expandedbed digester for the treatment of swine slurries.
Sorlini, C, Ranalli, G, Balsari, Pp. 351
Education in biotechnology at the pre-university level
Experimental gene technology at secondary schools inDenmark.
Fredtoft, E, Hansen, G, Johnsen, Kp. 354
Microcosmos: A necessary strategy for biotechnologyeducation.
Zook, DPp. 358
Education at the "H6here Bundes- Lehr- und Versuchs-anstalt fur Chemische Industrie - Vienna".
Frenzl, M, Berner, Rp. 360
Education in biotechnology at polytechnics in The
Netherlands.
Bresters, TW p. 364
Post-graduate biotechnology education at the Dutch
polytechnics.Dieteren, HML p. 368
Transgenic plants - new sources for food and agroindustrial
production
Introduction of virus resistance in potato cultivars.
van den Elzen, PJM, Huisman, MJ, Posthumus, D, Molendijk,L, Cornelissen, BJC, Hoekema, A, Jongedijk, E p. 372
Biological nitrification and denitrification
Biological nitrification and denitrification.
Hollo, J, Czako, L, Mihaltz, P p. 373
Microbial biomass and activity measurements: Their
role in describing the processes.Lindgaard-J0rgensen, P p. 380
The influence of the recycle rate on the performance of
a biological denitrification process in a fluidized bed.
Braun, R, Reisinger, C, Moser, A p. 384
Anoxic ammonium oxidation.
van de Graaf, AA, Mulder, A, Slijkhuis, H, Robertson, LA,
Kuenen, JG p. 388
Nitrification with immobilized bacteria in airlift
loop reactors: Modelling and application.Wijffels, RH, de Gooijer, CD, Tramper, J, Hunik, JH p. 392
Aerobic nitrate and nitrite reduction in continuous
cultures of Escherichia coli E4.
Brons, HJ, Zehnder, AJB p. 396
New developments in genetic diagnostics
New developments in genetic diagnostics.
Schwartz, M p. 402
Polymerase chain reaction (PCR) as a tool for studyingmitochondrial DNA mutations in human disease.
N0rby, S, Lestienne, P, Nelson, I p. 406
Rapid analysis of an aberrant region of the ribosomal
operon from a selection of clostridial species usingPCR: A new taxonomic approach.Colleran, GJ, Barry, T, Powell, R, Gannon, F,
Dunican, LK p. 410
Milk protein genotype mapping.
Lien, S, Brovold, MJ, Steine, T, Rogne, S, Molland,
TS, Vegarud, G, Alestrdm, P, Langsrud, T p. 414
Genotyping of apolipoprotein E by nucleotide sequenceand nucleotide incorporation analysis of affinity-
captured amplified DNA.
Syvanen, A-C, Harju, L, Soderlund, H P- 419
Bioorqanic synthesis
Recent advances in the use of biotransformation for
the synthesis of chiral, biologically active natural
products and selected analogues.
Roberts, SM P- 423
Synthesis of L-valine with NADH-regenerating systems:From enzymatic synthesis to bioconversion.
Honorat-Pascal, A, Monot, F, Ballerini, D, Vandecastee-
le, J-P P- 425
Enzymatic single-step processes for the production of
dipeptides from simple starting materials.
Aasmul-Olsen, S, Thorbek, P, Hansen, S, Widmer, F p. 429
Strategies in the preparation of homochiral compoundsusing combined enantioselective enzymes.
Straathof, AJJ, Rakels, JLL, Heijnen, JJ p. 433
Enzymatic synthesis of esters.
Knez, Z, Habulin, M, Pecnik, S p. 437
Enzymatic synthesis of carbohydrate esters in 2-pyrro-lidone.
Janssen, AEM, Klabbers C, van t' Riet, K, Franssen, MCR . p. 441
Eucaryotic gene regulation
Control of transcription initiation in eucaryotes.Yaniv, M, Chouard, T, Tronche, F, Blumenfeld, M, Bach,I, Cereghini, S
p. 445
Liver gene expression: Cloning of abundant mRNA tran¬
scripts from the liver of Atlantic salmon (Salmo salar),identification of salmon apolipoprotein A-l gene.Powell, R, Byrnes, L, Gannon, F
p. 453
Structural analysis of human repetitive DNA.
Miinker, T, Hennig, J, Grzeschik, K-H, Olek, K, Driesel,AJ
p. 457
Gene expression in procaryotes
A Thermus sp. strain which produces starch-hydrolyzingenzymes, and molecular cloning of its pullulanase gene.Sashihara, N, Nakamura, N, Horikoshi, K p. 461
Translational regulation in bacteriophage M13: Applicationfor the regulated production of heterologous proteinsin Escherichia coli.
Zaman, G, Schoenmakers, J, Konings, R p. 465
DNA sequence specificity of DE0P1 operator - DEOR re¬
pressor recognition in Escherichia coli.
Hammer, K, Bech, L, Dandanell, G, Hobolth, P p. 468
Recombinant protein expression in Escherichia coli
using the oxygen-dependent promoter of the vitreo-
scilla hemoglobin gene.
Khosla, C, Curtis, JE, Galazzo, J, Bailey, JE p. 472
Monitoring and controlling animal cells in bioreactors
The monitoring and control of animal cell cultures.
Spier, RE p. 476
Control of surfaces for anchorage dependent cell growth.Maroudas, NG p. 482
Monitoring nutrient components, metabolites, products,concentrations, and the behavior of animal cells in
bioreactors.
Schiigerl, K, Graf, H, Kretzmer, G, Freitag, R, Scheper,T, Wentz, D p. 486
The derivation and use of mathematical models for the
improvement of animal cell culture performance.
Engasser, J-M, Marc, A, Mailly, E, Fonteix, C, Goergen,
J-L, Martial, A, Ghoul, M p. 490
Protein engineering in relation to biological products
Structure-function studies of Trichoderma reesei cel-
lulases.
Ruohonen, L, Reinikainen, T, Jones, TA, Knowles, J,
Teeri, T p. 494
The rationel modification of porcine phospholipase A2.
Collins, M, Pickersgill, RW, Sumner, IG, Verheij, H,
de Haas, G, Goodenough, PW, Perry, BN p. 498
Cloning and mutagenesis of cysteine proteases.Collins, M, Revell, D, Sumner, I, Pickersgill, RW,
Goodenough, P p. 502
Enzymatic characterization of three genetically en¬
gineered variants of t-PA.
Sterky, C, Attersand, A, Nyberg, E, Lowenadler, B,
Hansson, L, Pohl, G p. 505
Protein engineering of glucoamylase from Aspergillus
niger. Studies of the enzymatic mechanism.
Sierks, MR, Svensson, B p. 509
Membrane technology for the separation of bioproducts
Synthetic membranes in biotechnology: Realities and
possibilities.
Belfort, G P- 513
Production of lactic acid by a complex processing of
agricultural lignocellulosic materials.
Rychtera, M, Machek, F, Pospichalova, V, Melzoch, K,Basarova G, Martinkova, L, Kratochvil, Z p. 517
Emulsion liquid membrane extractions in biotechnology:Separation of organic acids.
Chaudhuri, JB, Pyle, DL p. 521
Affinity (cross-flow)-microfiltration membranes for
the isolation of enzymes from crude extracts.
Champluvier, B, Briefs, G, Kula, M-R p. 525
Sensors using biological reactions
Recent trends in biosensor development.Mattiasson, B
p. 529
Application of a general sampling system for continuous
monitoring in biological systems using biosensors.
Hakansson, H, Hoist, 0, Mattiasson, B p. 535
Potential applications of biochemical fuel cells as
analytical tools.
Sell, D, Kreysa, G, Kraemer, P p. 539
On-line detection of lipase activity by flow injectionzymography.
Erdmann, H, Chemnltius, GC, Schmid, RDp. 544
Development of glucose micro-electrodes for measurementsin biofilms.
Cronenberg, CH, van den Heuvel, H, van der Hauw, M,van Groen, B
p. 548
Biohydrometallurgy
Biohydrometallurgy: Developments, potentials and trends.Rossi, G
p. 552
Microbial desulfurization of coal: Oxygen and carbondioxide mass transfer as a function of the tempera¬ture and the coal-slurry density.Boogerd, FC, Boon, M, Meeder, TA, Bos, P p. 558
Biosorption of molybdenum by biomass of microorganisms.Korenevsky, AA, Karavaiko, GI
, p. 561
A common plasmid (pTFO) in Thiobacillus ferrooxidans
strains from different geographical areas.
Polidoro, M, Buonfiglio, V, Valenti, P, Orsi, N p. 565
Leaching with fungi: Solubilization of metals from ores
and industrial wastes by means of organic acids.
Burgstaller, W, Strasser, H, Schinner, F p. 569
Possibilities and pitfalls during the establishment of
biotechnological enterprises
Possibilities and pitfalls during the establishment of
new biotechnological enterprises.Kooreman, HJ p. 573
EuroCetus: Establishment of a European biotechnology
company.
Hersbach, GJM p. 576
Presentation of Centocor.
Schoemaker, H p. 581
Extremophiles
DNA polymerases from thermophilic archaebacteria.
Elie, C, Hamal, A, Baucher, M-F, Forterre, P p. 589
The use of dehydrogenases in biotechnology: The prospects
provided by thermophilic enzymes in coenzyme regenerationsystems.
Bartolucci, S, Guagliardi, A, Raia, CA, Btickmann, AF,
Rossi, M p. 592
Hyperthermoactive amylases from the archaebacteria
Pyrococcus woesei and Pyrococcus furiosus.
Antranikian, G, Koch, R, Spreinat, A, Lemke, K p. 596
Biotechnological applications of the halophile bacteria.
Ramos-Cormenzana, A p. 600
Metabiosis of haloalkaliphilic bacteria.
Zvyagintseva, I, Kevbrina, M, Kostrikina, N, Skvortsova,
I, Kulichevskaya, I, Tarasov, A p. 604